This research aims to identify the BMP-15 gene in Sumba Ongole (SO) cattle (Bos indicus) which were reared extensively in Sumba Island. This gene has been known for its function in regulating reproductive performance in cows. The blood sample was collected from 161 SO cattle. The first step was DNA isolation from blood and the second step was electrophoresis and extraction. It then continued with optimization of annealing temperature, amplification, and sequencing. The results of sequencing were analyzed using a software program, Basic Local Alignment Search Tool (BLAST). The annealing temperature optimization results obtained temperature of 58 °C was the proper temperature to amplify DNA. All samples studied with BMP-15 gene amplification gained results of 350 bp. Results of BLAST program analysis and Mega 6.01 program showed a close genetic relationship, characterized by the result sequence of BMP-15 of SO cattle possessing proximity to other ruminants, such as Bos taurus and Capra hircus breed. Based on this study, it could be concluded that the BMP-15 gene amplified successfully of 350 bp and has the adjacency of nucleotide sequence with the BMP 15 gene in some other types of ruminants.

African Swine Fever (ASF) is an important infectious disease in pigs caused by African Swine Fever Virus (ASFV). Despite not being zoonotic, this disease has the potential to severely affect the socioeconomic conditions in the impacted regions. The majority of pig farmers in Indonesia, particularly those in Kupang City, that raise pigs in backyards or on a small scale, experience the impact of ASF's effects. Early in 2020, the ASF cases were confirmed in Timor Island, including the Kupang City area in Nusa Tenggara Timur (NTT) Province. The molecular information on ASFV in this area is still limited. In order to determine the homology and nucleotide analysis using BLAST NCBI, the ASFV p72 (B646L) gene fragment sequence from Kupang City was compared to ASFV p72 (B646L) gene segments from other parts of Indonesia and several other Asian countries. The results of nucleotide analysis and sequence homology of the original ASFV p72 (B646L) gene fragment from Kupang City showed a high level of homology to the ASFV p72 (B646L) gene fragment from West Java, North Sumatra, and several Asian countries. The findings from this study indicate that the source of ASF viral transmission across different regions may be comparable. Therefore, to prevent the dissemination of ASF, strict biosecurity measures must be implemented along with monitoring of animal and product transportation.

Tulisan mendeskripsikan pengetahuan masyarakat sasaran sosialisasi tentang implementasi kesrawan dan praktik higiene/sanitasi penyembelihan hewan qurban di Kota Kupang setelah mendapatkan penyuluhan dan pelatihan oleh tim pengabdian kepada masyarakat Universitas Nusa Cendana. Penelitian ini merupakan penelitian survey pada populasi yang diintervensi dengan penyuluhan dan pelatihan dengan menggunakan metode pre test dan post test. Peserta penyuluhan dan pelatihan kesrawan dan higiene daging qurban di Masjid Al Mujahidin Penfui dan Masjid Darul Hijrah Kolhua, Kota Kupang (masing-masing 15 orang), yaitu. Soal pretest dan posttest sebanyak 20 pertanyaan benar dan salah. Data dianalisis secara deskriptif dengan menggunakan Microsoft Excel. Kegiatan penyuluhan dan pelatihan kesrawan dan praktik higiene bagi panitia qurban di kedua masjid berhasil meningkatkan skor pemahaman peserta tentang aspek kesrawan dan praktik higiene dan sanitasi dalam menangani hewan dan daging qurban sebesar 47,5% dan 40,2%. Pemahaman tersebut penting sebagai landasan sikap dan tindakan dalam menghasilkan daging qurban yang aman, sehat, utuh dan halal.

Hematological parameters have provided useful information about the animal health status. The main objective of the study was to measure the hematological profile of Sumba Ongole (SO) cattle extensively reared in semi-arid land in Sumba, NTT. Blood samples from seventy-five animals were examined to measure its hematological parameters based on age and sex. Animal were grouped based on sex into male and female in the age groups 1.5 to 3 years (n=42), 3.5 to 4 years (n=23) and ≥ 5 years (n=10). Mean standard error values of all the hematological parameters for The SO cattle, both male and female cattle at different age of group were measured. Several hematological parameters, such as erythrocytes, platelets, hemoglobin, hematocrit and mean corpuscular volume (MCV) level were significantly different (p<0.05) between male and female, while erythrocyte, hemoglobin, hematocrit, MCV were significantly different (p<0.05) within 3 groups of age. These results would contribute to better understanding of the hematological indicators for estimating the physiological status of Sumba Ongole cattle in Sumba and it can be used as valuable information for beef cattle reared with similar environmental condition, especially in semi-arid tropical climate.

African swine fever (ASF) is a destructive re-emerging swine disease that has posed a serious economic threat to the global pig farming sector. In past years, ASF has rapidly spread over Europe, Asia, and Oceania, and begin to enter Indonesia in the middle of 2019. The clinical and pathological symptoms of ASF are influenced by the strain's virulence, the transmission pathway, and the pig's immunological and health status. ASF’s clinical manifestations are known to evolve, from after an invasion enters a new free region to after the disease has been established in the territory for a longer period. Identifying ASF clinical signs and pathological changes is crucial for a comprehensive and reliable early detection system. The objective of this research is to observe and identify gross pathology in ASF suspect pigs in order to obtain a better understanding of the cause of death. Two dead pigs from a farm in Oebelo village, Kupang regency, Indonesia with a recent history of massive deaths had been examined in this study. The post-mortem results showed that hemorrhagic splenomegaly and hemorrhagic lymphadenitis were the main lesions observed at the examinations. Furthermore, hemorrhages were also found in various internal organs such as the kidneys, liver, and heart. To determine the exact cause of the pigs' deaths, a molecular diagnostic test should be conducted.

A study was carried out in one of Indonesia native cattle, Sumba Ongole (SO) Cattle for determination of statuses of various biochemical parameters, aspartate aminotransferase (AST or SGOT) and alanine aminotransferase (ALT or SGPT). Apparently blood from randomly selected cattle (n= 36) were collected aseptically. Biochemical analysis of AST and ALT was performed on UPT. Laboratorium Kesehatan, Kupang, NTT. The results showed that the variations of parameters were not significant (p>0.05) between male and female SO cattle. The AST/SGOT (142.56±20.55 U/l) was slightly higher in female compared with the male (137.00±16.24 U/l), while level of ALT/SGPT were 46.44±10.99 U/l in male and 46.41 ± 10.23 U/l in female. Although, the result was not significant (p>0.05), level of AST and ALT in SO cattle was higher compared to standard which is 60-125 U/l for AST and 6.9-35 U/l for ALT. Results showed possibility of seasonal effect on AST/ SGOT and ALT/SGPT level in male and female SO cattle.

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The purpose of this study was to determine the effect of soursop leaf (Annona muricata), saffron white (Curcuma mangga Val.) and cherry leaves (Muntingia calabura L.) and theirs combinations in drinking water on performances and blood cholesterol  of  laying roosters that infected by Escherichia coli. This study was designed using  completely randomized design (CRD) with 11 treatments and 3 replications and each treatment using the 4 roosters. That obtained 33 experimental units. The treatments were the drinking water contain decoction of  soursop leaves (DS), saffron white (KP) and cherry leaves (DK), and combinations were as follows: R0: without decoction of herbs (control), R1: 6% of DS decoction, R2: 6% of KP decoction, R3: 6 % of  DK decoction, R4: 2% of  DS decoction + 4% of KP decoction, R5: 2% of  DS decoction + 4% of DK decoction, R6: 2%  of KP decoction + 4% of DS decoction, R7: 2% of KP decoction  + 4% of DK decoction, R8: 2% of DK decoction + 4% of DS decoction, R9: 2% of  DK decoction + 4% of KP decoction, R10: 2%  of DS decoction + 2% of KP decoction + 2% of DK decoction. Parameters of  performance were: feed consumption, water intake, body weight gain, feed conversion. The result showed that the treatment had no effect on the performances (feed consumption, water consumption, body weight gain, feed conversion).