Blood AST and ALT profile of Sumba Ongole cattle

Gaina, Cynthia Dewi; Sanam, Maxs U. E.; Nalley, Wilmientje Marlene; Benu, Imanuel; Saputra, Agus

doi:10.29244/avl.4.1.17-18

Cited by 5 publications

(4 citation statements)

References 5 publications

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“…Hematological status also provides information regarding physiological conditions, animal health Jurnal Medik Veteriner Dwi Kristanto, et al J Med Vet 2023, 6(2):237243. pISSN 2615-7497; eISSN 2581-012X | 238 status (Delfino et al, 2012;Klinkon and Ježek, 2012;Roland et al, 2014), evaluation of reproductive performance (Yuherman et al, 2017;Abramowicz et al, 2019), determining nutritional status and livestock management (Gaina et al, 2019;Yuherman et al, 2017), determining disease status, therapeutic actions and monitoring of disease management (Abramowicz et al, 2019;Kim et al, 2020), observation of stress levels (Delfino et al, 2014;Roberto et al, 2010;Rodrigues et al, 2010) and the effect on animal welfare (Gaina et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Hematological Levels of Simmental-Ongole Crossbreed (SimPO) and Ongole Crossbreed (PO) Cattle Reared Semi-Intensively

Kristanto,

Septiyani

2023

J. Med. Vet.

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Hematological analysis in cattle is an important basis for determining physiological conditions, biomedical study, and veterinary clinical diagnosis. This study aimed to determine the comparison of hematological values between Simmental-Ongole crossbreed (SimPO) and Ongole crossbreed (PO) cattle reared semi-intensively in oil palm plantations area. A total of 70 blood samples were collected from 35 female SimPO and 35 female PO cattle aged ± 3,5 years who reared from the same area. Blood samples were collected via the coccygeal vein as much as 3 ml, performed at 07.00 in the morning before being grazed on oil palm plantations. Blood samples were collected using a vacutainer tube containing Ethylene Diamine Tetra-Acetic Acid (EDTA) for further analysis using a hematology analyzer. Data from hematological examination results were then analyzed using an independent sample t-test with a significance level of p < 0,05. In results, reported that there were no significant differences in several hematological parameters that have been analyzed. We can conclude that there was no substantial hematological level in the SimPO and PO cattle reared semi-intensively on oil palm plantations.

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Section: Introductionmentioning

confidence: 99%

Comparison of Hematological Levels of Simmental-Ongole Crossbreed (SimPO) and Ongole Crossbreed (PO) Cattle Reared Semi-Intensively

Kristanto,

Septiyani

2023

J. Med. Vet.

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“…Sheep and goats, like the Small Tail Han sheep, Chinese goats (Wang et al, 2011), Iranian Baluchi sheep (Moradband et al, 2011), Xinjiang Cele Black sheep (Niu et al, 2021) and Markhoz goats (Ghoreishi et al, 2019;Pourali et al, 2020) are susceptible to having low birth rates because of Polymorphism of the BMP 15 Gene in Sumba Ongole (Bos indicus) Cattle probably be different from other Ongole breeds, which are extensively grazed under a harsh environment in a tropical semi-arid region, just like in Sumba Island. PO cattle are crossbreds of Java cattle and Sumba Ongole (SO) cattle, with SO cattle being one of Indonesia's native beef cows, introduced straight from India by the Indonesian government and extensively grazed on Sumba Island for genetic development (Gaina et al, 2020). They are well-known for their protein content and for being very adaptable to severe conditions, as seen on Sumba Island (Gaina et al, 2019;Sutarno and Setyawan, 2015).…”

Section: Introductionmentioning

confidence: 99%

The Potency of the BMP 15 Gene as a Marker of Reproductive Traits in Sumba Ongole (Bos indicus) Cattle

Gaina¹,

Sanam²,

Nalley³

et al. 2023

ASD

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Background: As one of the important genetic resources for Ongole beef cattle, the study aimed to identify any potential polymorphisms in the Bone Morphogenetic Protein 15 gene in a group of Sumba Ongole (SO) cattle. Methods: Blood samples from 161 SO cattle were obtained and examined. The initial stage was the isolation of deoxyribonucleic acid (DNA). The next procedures were electrophoresis and extraction, followed by annealing temperature optimization, amplification and sequencing. The Basic Local Alignment Search Tool (BLAST) was used to examine the sequencing findings. The annealing temperature was 58°C for 15 seconds, with an amplification gain of 350 base pairs (bp). Result: The result showed that there were six single nucleotide polymorphisms (SNPs) of the BMP 15 gene in this region. Six mutations were SNP c.57 G greater than A, SNP c.120 C greater than T, SNP c.338 G greater than T, SNP c.360 C greater than A, SNP c.361 G greater than T, SNP c.365 A greater than T. The polymorphic informative content (PIC) value (0.25 less than =PIC less than =0.5) was reached from low (0.02) to moderate high (0.45 and 0.47). A total of 2 SNPs in the BMP 15 gene with moderate-high PIC value was c.360 C greater than A and c.365 A greater than T. In conclusion, we reported for the first time the polymorphism of the BMP 15 gene which is required for further investigation as to whether this polymorphic could be responsible for reproductive trait variations in Ongole cattle.

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“…Previous studies have mentioned the genetic variations in BMP-15 gene in goat and cow, for example in Chinese goat [8] and Iranian Baluchi Sheep [9]. The SO cattle are categorized as Bos indicus cattle that are one of Indonesia's native beef cows that have been known for its benefit as a source of protein and highly adaptive to extreme climates, just like in Sumba Island [10,11]. Many genes are known to affect reproductive performance traits, but the role of the BMP-15 gene, specifically in SO cattle has not been explored yet.…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification of Bone Morphogenetic Protein-15 (BMP-15) gene of Sumba Ongole cattle

Gaina¹,

Sanam²,

Nalley³

et al. 2022

Advances in Biological Sciences Research

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This research aims to identify the BMP-15 gene in Sumba Ongole (SO) cattle (Bos indicus) which were reared extensively in Sumba Island. This gene has been known for its function in regulating reproductive performance in cows. The blood sample was collected from 161 SO cattle. The first step was DNA isolation from blood and the second step was electrophoresis and extraction. It then continued with optimization of annealing temperature, amplification, and sequencing. The results of sequencing were analyzed using a software program, Basic Local Alignment Search Tool (BLAST). The annealing temperature optimization results obtained temperature of 58 °C was the proper temperature to amplify DNA. All samples studied with BMP-15 gene amplification gained results of 350 bp. Results of BLAST program analysis and Mega 6.01 program showed a close genetic relationship, characterized by the result sequence of BMP-15 of SO cattle possessing proximity to other ruminants, such as Bos taurus and Capra hircus breed. Based on this study, it could be concluded that the BMP-15 gene amplified successfully of 350 bp and has the adjacency of nucleotide sequence with the BMP 15 gene in some other types of ruminants.

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Blood AST and ALT profile of Sumba Ongole cattle

Cited by 5 publications

References 5 publications

Comparison of Hematological Levels of Simmental-Ongole Crossbreed (SimPO) and Ongole Crossbreed (PO) Cattle Reared Semi-Intensively

Comparison of Hematological Levels of Simmental-Ongole Crossbreed (SimPO) and Ongole Crossbreed (PO) Cattle Reared Semi-Intensively

The Potency of the BMP 15 Gene as a Marker of Reproductive Traits in Sumba Ongole (Bos indicus) Cattle

Molecular Identification of Bone Morphogenetic Protein-15 (BMP-15) gene of Sumba Ongole cattle

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