Molecular Identification of Bone Morphogenetic Protein-15 (BMP-15) gene of Sumba Ongole cattle

Abstract

This research aims to identify the BMP-15 gene in Sumba Ongole (SO) cattle (Bos indicus) which were reared extensively in Sumba Island. This gene has been known for its function in regulating reproductive performance in cows. The blood sample was collected from 161 SO cattle. The first step was DNA isolation from blood and the second step was electrophoresis and extraction. It then continued with optimization of annealing temperature, amplification, and sequencing. The results of sequencing were analyzed using a software program, Basic Local Alignment Search Tool (BLAST). The annealing temperature optimization results obtained temperature of 58 °C was the proper temperature to amplify DNA. All samples studied with BMP-15 gene amplification gained results of 350 bp. Results of BLAST program analysis and Mega 6.01 program showed a close genetic relationship, characterized by the result sequence of BMP-15 of SO cattle possessing proximity to other ruminants, such as Bos taurus and Capra hircus breed. Based on this study, it could be concluded that the BMP-15 gene amplified successfully of 350 bp and has the adjacency of nucleotide sequence with the BMP 15 gene in some other types of ruminants.

Integrated genome based evaluation of safety and probiotic characteristics of Lactiplantibacillus plantarum YW11 isolated from Tibetan kefir

“…These modifications might be related to the host or to the geological and geographical environment in which they dwell. The taxa of L. plantarum exhibit greater genomic variety than previously believed, according to earlier findings from pangenome research (Kamau et al, 2020;Gaina et al, 2022;Li et al, 2022). A closed pangenome is regarded as a finalized pangenome in which the number of genomes does not change even if new genomes are added to it, as opposed to an open pangenome, that expands every time a new genome is added.…”

“…As a result, they have a restricted selection of genes available to them. Examples of closed pangenomes are Mycobacterium TB, Bacillus anthracis, and Chlamydia trachomatis ( Gaina et al, 2022 ; Li et al, 2022 ; Liu et al, 2022 ; Surve et al, 2022 ; Syrokou et al, 2022 ).…”

“…These genes are also responsible to produce vitamins. Using a piece of software known as the Basic Local Alignment Search Tool (BLAST), 3 we were able to match the protein sequences of these genes with the genome that we are now researching ( Gaina et al, 2022 ). The alignment has to achieve at least 70% identity and a cutoff of 1E5 to be successful.…”

“…(A) L. plantarum strains were shown to be effective probiotics and revealed a connection to other clades (B) the gene counts distribution and length distribution analysis. (Gaina et al, 2022;Li et al, 2022;Liu et al, 2022;Surve et al, 2022;Syrokou et al, 2022). The gastrointestinal tract includes the oral cavity, large intestine, stomach, and small intestine of the human are some of the locations where L. plantarum YW11 may be found.…”