Manipulating P4 concentrations before AI has the potential to improve pregnancy outcomes to AI in B. indicus females, but treatment may need to vary between animals classified as anovulatory or ovulatory.
Penelitian mengenai studi performa umum dan status fisiologis kuda Sumba di dilaksanakan pada tanggal April-September 2018 bertempat di Peternakan kuda sumba, Pulau Sumba, NTT. Tujuan penelitian ini adalah untuk mengetahui performa umum kuda sumba dalam kaitannya dengan status fisiologisnya. Penelitian yang digunakan adalah metode deskriptif yaitu metode yang menggambarkan objek sesuai dengan apa adanya, pengambilan sampel dilakukan selama dua minggu. Dalam penelitian ini peubah yang diamati adalah frekuensi respirasi, frekuensi denyut jantung dan suhu tubuh yang dilakukan pada 30 ekor indukan kuda sumba memiliki riwayat pernah digunakan untuk olahraga kuda pacuan baik kuda dengan jenis kelamin jantan dan betina dan 5 ekor anakan. Hasil dari penelitian tersebut disimpulkan bahwa frekuensi respirasi indukan memiliki nilai rataan 33±6.7 per menit dan anak memiliki nilai rataan 44±5.7 per menit. Frekuensi denyut jantung indukan memiliki nilai rataan 44±8.0 per menit dan 62±2.8 per menit . Suhu tubuh kuda indukan memiliki rataan yaitu 37.5±0.60C dan suhu anak memiliki rataan yaitu 38.2 ± 0.30C. Selain itu, hasil penelitian menunjukkan bahwa kuda indukan memiliki rataan tinggi badan 138.8 ±8.4 cm dan anakan memiliki rataan 106.2 ± 7.1 cm; indukan memiliki rataan panjang badan 139.4 ± 9.0 cm dan anakan rataan 94.4 ± 3.0; indukan memiliki rataan lebar dada 41.3 ±57.1 cm dan anakan memiliki rataan lebar dada 21.2 ± 3.1 cm; indukan memiliki tinggi punggung 133.9 ± 8.8 cm dan anak memiliki rataan tinggi punggung 104.4 ± 8.6 cm dan indukan memiliki panjang bahu 58.5 ± 5.5 cm dan anak memiliki rataan panjang bahu 40.0 ±5.6 cm
This research aims to identify the BMP-15 gene in Sumba Ongole (SO) cattle (Bos indicus) which were reared extensively in Sumba Island. This gene has been known for its function in regulating reproductive performance in cows. The blood sample was collected from 161 SO cattle. The first step was DNA isolation from blood and the second step was electrophoresis and extraction. It then continued with optimization of annealing temperature, amplification, and sequencing. The results of sequencing were analyzed using a software program, Basic Local Alignment Search Tool (BLAST). The annealing temperature optimization results obtained temperature of 58 °C was the proper temperature to amplify DNA. All samples studied with BMP-15 gene amplification gained results of 350 bp. Results of BLAST program analysis and Mega 6.01 program showed a close genetic relationship, characterized by the result sequence of BMP-15 of SO cattle possessing proximity to other ruminants, such as Bos taurus and Capra hircus breed. Based on this study, it could be concluded that the BMP-15 gene amplified successfully of 350 bp and has the adjacency of nucleotide sequence with the BMP 15 gene in some other types of ruminants.
Hematological parameters have provided useful information about the animal health status. The main objective of the study was to measure the hematological profile of Sumba Ongole (SO) cattle extensively reared in semi-arid land in Sumba, NTT. Blood samples from seventy-five animals were examined to measure its hematological parameters based on age and sex. Animal were grouped based on sex into male and female in the age groups 1.5 to 3 years (n=42), 3.5 to 4 years (n=23) and ≥ 5 years (n=10). Mean standard error values of all the hematological parameters for The SO cattle, both male and female cattle at different age of group were measured. Several hematological parameters, such as erythrocytes, platelets, hemoglobin, hematocrit and mean corpuscular volume (MCV) level were significantly different (p<0.05) between male and female, while erythrocyte, hemoglobin, hematocrit, MCV were significantly different (p<0.05) within 3 groups of age. These results would contribute to better understanding of the hematological indicators for estimating the physiological status of Sumba Ongole cattle in Sumba and it can be used as valuable information for beef cattle reared with similar environmental condition, especially in semi-arid tropical climate.
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