This research aims to identify the BMP-15 gene in Sumba Ongole (SO) cattle (Bos indicus) which were reared extensively in Sumba Island. This gene has been known for its function in regulating reproductive performance in cows. The blood sample was collected from 161 SO cattle. The first step was DNA isolation from blood and the second step was electrophoresis and extraction. It then continued with optimization of annealing temperature, amplification, and sequencing. The results of sequencing were analyzed using a software program, Basic Local Alignment Search Tool (BLAST). The annealing temperature optimization results obtained temperature of 58 °C was the proper temperature to amplify DNA. All samples studied with BMP-15 gene amplification gained results of 350 bp. Results of BLAST program analysis and Mega 6.01 program showed a close genetic relationship, characterized by the result sequence of BMP-15 of SO cattle possessing proximity to other ruminants, such as Bos taurus and Capra hircus breed. Based on this study, it could be concluded that the BMP-15 gene amplified successfully of 350 bp and has the adjacency of nucleotide sequence with the BMP 15 gene in some other types of ruminants.
African Swine Fever (ASF) is an important infectious disease in pigs caused by African Swine Fever Virus (ASFV). Despite not being zoonotic, this disease has the potential to severely affect the socioeconomic conditions in the impacted regions. The majority of pig farmers in Indonesia, particularly those in Kupang City, that raise pigs in backyards or on a small scale, experience the impact of ASF's effects. Early in 2020, the ASF cases were confirmed in Timor Island, including the Kupang City area in Nusa Tenggara Timur (NTT) Province. The molecular information on ASFV in this area is still limited. In order to determine the homology and nucleotide analysis using BLAST NCBI, the ASFV p72 (B646L) gene fragment sequence from Kupang City was compared to ASFV p72 (B646L) gene segments from other parts of Indonesia and several other Asian countries. The results of nucleotide analysis and sequence homology of the original ASFV p72 (B646L) gene fragment from Kupang City showed a high level of homology to the ASFV p72 (B646L) gene fragment from West Java, North Sumatra, and several Asian countries. The findings from this study indicate that the source of ASF viral transmission across different regions may be comparable. Therefore, to prevent the dissemination of ASF, strict biosecurity measures must be implemented along with monitoring of animal and product transportation.
Tulisan mendeskripsikan pengetahuan masyarakat sasaran sosialisasi tentang implementasi kesrawan dan praktik higiene/sanitasi penyembelihan hewan qurban di Kota Kupang setelah mendapatkan penyuluhan dan pelatihan oleh tim pengabdian kepada masyarakat Universitas Nusa Cendana. Penelitian ini merupakan penelitian survey pada populasi yang diintervensi dengan penyuluhan dan pelatihan dengan menggunakan metode pre test dan post test. Peserta penyuluhan dan pelatihan kesrawan dan higiene daging qurban di Masjid Al Mujahidin Penfui dan Masjid Darul Hijrah Kolhua, Kota Kupang (masing-masing 15 orang), yaitu. Soal pretest dan posttest sebanyak 20 pertanyaan benar dan salah. Data dianalisis secara deskriptif dengan menggunakan Microsoft Excel. Kegiatan penyuluhan dan pelatihan kesrawan dan praktik higiene bagi panitia qurban di kedua masjid berhasil meningkatkan skor pemahaman peserta tentang aspek kesrawan dan praktik higiene dan sanitasi dalam menangani hewan dan daging qurban sebesar 47,5% dan 40,2%. Pemahaman tersebut penting sebagai landasan sikap dan tindakan dalam menghasilkan daging qurban yang aman, sehat, utuh dan halal.
African swine fever (ASF) is a destructive re-emerging swine disease that has posed a serious economic threat to the global pig farming sector. In past years, ASF has rapidly spread over Europe, Asia, and Oceania, and begin to enter Indonesia in the middle of 2019. The clinical and pathological symptoms of ASF are influenced by the strain's virulence, the transmission pathway, and the pig's immunological and health status. ASF’s clinical manifestations are known to evolve, from after an invasion enters a new free region to after the disease has been established in the territory for a longer period. Identifying ASF clinical signs and pathological changes is crucial for a comprehensive and reliable early detection system. The objective of this research is to observe and identify gross pathology in ASF suspect pigs in order to obtain a better understanding of the cause of death. Two dead pigs from a farm in Oebelo village, Kupang regency, Indonesia with a recent history of massive deaths had been examined in this study. The post-mortem results showed that hemorrhagic splenomegaly and hemorrhagic lymphadenitis were the main lesions observed at the examinations. Furthermore, hemorrhages were also found in various internal organs such as the kidneys, liver, and heart. To determine the exact cause of the pigs' deaths, a molecular diagnostic test should be conducted.
Hematological parameters have provided useful information about the animal health status. The main objective of the study was to measure the hematological profile of Sumba Ongole (SO) cattle extensively reared in semi-arid land in Sumba, NTT. Blood samples from seventy-five animals were examined to measure its hematological parameters based on age and sex. Animal were grouped based on sex into male and female in the age groups 1.5 to 3 years (n=42), 3.5 to 4 years (n=23) and ≥ 5 years (n=10). Mean standard error values of all the hematological parameters for The SO cattle, both male and female cattle at different age of group were measured. Several hematological parameters, such as erythrocytes, platelets, hemoglobin, hematocrit and mean corpuscular volume (MCV) level were significantly different (p<0.05) between male and female, while erythrocyte, hemoglobin, hematocrit, MCV were significantly different (p<0.05) within 3 groups of age. These results would contribute to better understanding of the hematological indicators for estimating the physiological status of Sumba Ongole cattle in Sumba and it can be used as valuable information for beef cattle reared with similar environmental condition, especially in semi-arid tropical climate.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.