Aim:This research was conducted to clarify the capability of human umbilical mesenchymal stem cells conditioned medium (HU-MSCM) to promote regenerations of primary wound healing on the incision skin injury.Materials and Methods:In this study, two approaches in vitro and in vivo already done. On in vitro analysis, tube formation was performed using HU vein endothelial cells in the presence of HU-MSCM, in some experiments cells line was incubated prior the presence of lipopolysaccharide and HU-MSCM then apoptosis assay was performed. Furthermore, in vivo experiments 12 female rats (Rattus norvegicus) were used after rats anesthetized, 7 mm wound was made by incision on the left side of the body. The wound was treated with HU-MSCM containing cream, povidone iodine was run as a control. Wound healing regenerations on the skin samples were visualized by hematoxylin-eosin staining.Results:In vitro models elucidate HU-MSCM may decreasing inflammation at the beginning of wound healing, promote cell migration and angiogenesis. In addition in vivo models show that the incision length on the skin is decreasing and more smaller, HE staining describe decreasing of inflammation phase, increasing of angiogenesis, accelerate fibroplasia, and maturation phase.Conclusions:Taken together our observation indicates that HU-MSCM could promote the acceleration of skin tissue regenerations in primary wound healing process.

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Aim:Various studies have shown that secreted factors alone in culture medium without stem cell are capable of repairing tissues by itself in various conditions involving damaged tissue/organ. Therefore, this study was aimed to investigate the role of human umbilical cord mesenchymal stem cell-derived conditioned medium (CM) on the recovery of pancreatic β-cells in Wistar rats (Rattus norvegicus) with type 1 diabetes mellitus.Materials and Methods:The 0.05 ml CM induction was applied to the diabetic group of rats in weeks 1, 2, 3, and 4. 1 week after each CM induction, insulin concentration was analyzed using ELISA. The pancreas was divided into 3 regions, processed by paraffin method, stained with hematoxylin-eosin, and immunohistochemical method for insulin.Results:This study indicated the decrease in the total number of islets and insulin concentration after the injection of single dose of alloxan. The exocrine acini were also damaged. Microscopic observation detected the presence of small islets in the diabetic group 1 week after the first 0.05 ml CM induction. The number and size of the islets increased in line with the CM doses and time of inductions. Immunohistochemically, the presence of low intensity of insulin-positive cells could be recognized at the splenic and duodenal regions of the pancreas, but not gastric region, 1 week after the first and second 0.05 ml CM induction. The intensity of staining and the number of insulin-positive cells increased dramatically in 1 week after the third and fourth 0.05 ml of CM induction in all regions of the pancreas. The data of insulin blood concentration showed clear differences between the second and the fourth induction of 0.05 ml CM induction.Conclusions:This study showed very strong evidence on the role of human umbilical cord mesenchymal stem cell-derived CM in recovering the pancreatic β-cells damage in Wistar rats (R. norvegicus) with type 1 diabetes mellitus, structurally and functionally.

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Background and Aim: Feline lower urinary tract disease (FLUTD) is one of the common cat diseases. The aim of this study was to detect feline idiopathic cystitis (FIC) as a cause of FLUTD in Sleman Regency, which is a problem in the population. Materials and Methods: Seventy-three cats with FLUTD symptoms were used from seven veterinary practices in Sleman Regency. The logging of each cat's medical history, clinical examination, urinalysis, routine blood screening, and ultrasonography was conducted to diagnose the cause of FLUTD. Results: The percentages of diseases causing FLUTD included FIC 21.9%, urolithiasis 57.5%, urinary tract infection (UTI) 16.4%, neoplasia 1.4%, trauma 1.4%, and nervous disorders 1.4%. Conclusion: FIC, one of the causes of FLUTD, is found in cats and has become a problem among the cat population in Sleman Regency, Yogyakarta, Indonesia. Various handling and preventive efforts should be undertaken against the disease.

Borax is a harmful compound to human health but sometimes was used illegally as preservative in meatball. Curcumax reagent is a compound which developed from the previous borax detector base on tumeric extract. This reagen was developed as an easy kit to apply in the field. This study aims were to determine accuracy of sight obesrvation and spectrophotometer to detected borax in boiled meatballs which tested by Curcumax and to determine the effect of boiling on the borax presence in the meatballs. This study was using meatballs group as a control and another meatballs group preserve with borax 0.5%. Each treatment group was boiled in 0, 5, 10, and 15 minutes and each treatment unit had three replications which consist five meatballs each of them. Borax detection was done by mixing 1ml Curcumax reagent into 1 ml meatball extract. Qualitative examination of borax presence in the meatballs was known based on the color changing of tested meatball's extract from yellow became orange (direct sight with eyes). The Quantitaive analysis was done by measured using spectrophotometer base on wave-length (λ) at 570 nm. Data was analyzed with ANOVA and continued by Least Significant Difference (LSD) analysis. Borax detection using curcumax based on sight sense against color change giving an inconsistence result. Meanwhile quantitative measurement by spectrophotometer more accurate than one. Curcumax had been detected borax in meatball which boiled for 15 minutes.Keywords : borax, meatballs, Curcumax, spectrophotometry AbstrakBoraks merupakan zat kimia yang berbahaya bagi kesehatan masyarakat namun seringkali digunakan dalam bakso. Pengembangan alat deteksi boraks yang praktis sangat diperlukan di lapangan. Reagen Curcumax adalah hasil pengembangan alat deteksi boraks yang berbahan dasar ekstrak kunyit. Penelitian ini bertujuan mengetahui ketelitian pengamatan indra penglihatan dan spektrofotometer dalam mendeteksi boraks dalam bakso yang direbus yang diuji menggunakan reagen Curcumax dan mengetahui pengaruh perebusan terhadap keberadaan boraks dalam bakso. Penelitian ini menggunakan kelompok bakso kontrol dan kelompok bakso boraks 0,5% dengan perlakuan perebusan selama 0, 5, 10 dan 15 menit. Setiap unit perlakuan dilakukan ulangan sebanyak 3 kali yang masingmasing tediri dari 5 butir bakso. Pemeriksaan kandungan boraks dilakukan dengan cara 1 ml ekstrak bakso diuji dengan 1 ml reagen Curcumax. Penilaian keberadaan boraks secara kualitatif didasarkan pada perubahan warna ekstrak bakso yang berubah dari kuning menjadi oranye. Pemeriksaan kuantitaif dilakukan dengan cara mengukur intensitas warna ekstrak bakso yang telah ditetesi Curcumax dengan spektrofotometer pada panjang gelombang 570 nm. Data dianalisis dengan ANOVA dan dilanjutkan dengan analisis Least Significant Difference(LSD). Penilaian kulitatif boraks berdasarkan pengamatan indra penglihatan memberikan hasil yang tidak konsisten sementara penilaian berdasarkan spektrofotometer lebih akurat. Perebusan mengurangi kandungan boraks dalam bakso. Reagen Curcumax ma...

Studies on antibiotic residues content in food of animal origin are currently needed to support veterinary public health programs. The present study was described bioassay method for the detection of antibiotic residues in chicken meat and eggs from traditional market at Yogyakarta City. A number of twenty-four chicken meat samples and 24 egg samples were taken from 8 traditional markets in Yogyakarta city. Samples were examined at Centre for Veterinary Wates, Yogyakarta, Indonesia using bioassay method for screening detection of penicillin, aminoglycoside, macrolide and tetracycline residues. This bioassay method using some bacteria, such as Bacillus stearothermophillus, B. cereus, B. subtilis, and Kocuria rizophila. A percentage of the results showed that 8.33% (2/24) samples of chickens tested positively contained the oxytetracycline antibiotic residues. Meanwhile, as much as 75% (18/24) samples of positive eggs contain penicillin antibiotic residues, positive residues of aminoglycoside amounted to 12.5% (3/24) and the positive residues of oxytetracycline also amounted to 12.5% (3/24).

ABSTRAKBerbagai penelitian menunjukkan bahwa ekstrak media penumbuh sel punca mesenkimal (EMP-SPM), tanpa sel punca itu sendiri, telah ditemukan terdapat berbagai faktor tropik hasil sekresi sel punca mesenkimal di dalam media kultur yang dapat meregenerasi jaringan yang rusak. Penelitian ini bertujuan untuk mengetahui gambaran kadar malondialdehid (MDA) dalam plasma dan ginjal tikus diabetes melitus tipe 2 (DMT2) dengan terapi EMPSPM. Dua puluh lima ekor tikus wistar digunakan dalam penelitian ini dibagi menjadi 5 kelompok yaitu kelompok DMT2 + 0,05 ml/kg BB EMPSPM (0,05); kelompok DMT2 + 0,1 ml/kg BB EMPSPM (0,1); kelompok DMT2 + 0,2 ml/kg BB EMPSPM (0,2), kelompok kontrol DMT2 (DMT2), dan kelompok sehat (KS). Induksi DMT2 dengan menggunakan streptozotosinnikotinamid (STZ-NA). Terapi mulai dilakukan pada hari ke 7 setelah kondisi DM tercapai, diberikan 4 kali dengan selang waktu 7 hari secara intraperitoneal. Data kadar glukosa darah dan MDA dianalisi secara statistik. Hasil penelitian menunjukkan bahwa induksi DMT2 dengan STZ-NA dapat menaikkan kadar glukosa dan MDA dalam darah (P<0,05). Terapi 0,05; 0,1; dan 0,2 ml/kg BB EMPSPM menunjukkan dapat menurunkan kadar glukosa darah, kadar MDA plasma dan ginjal (P<0,05). Kadar 0,2 ml/kg BB EMPSPM menunjukkan aktifitas lebih baik dalam menurunkan kadar glukosa darah dan MDA. Berdasarkan penelitian ini, EMPSPM dapat menurunkan kadar glukosa darah serta kadar MDA dalam darah dan ginjal tikus DMT2.Kata kunci: EMPSPM, streptozotosin, nikotinamid, diabetes mellitus tipe 2, malondialdehid ABSTRACTVarious studies show that Mesenchymal Stem Cell-Conditioned Media (MSC-CM)), without the stem cells themselves, has been found to contain various trophic factor secretion results in a mesenchymal stem cell culture medium that can regenerate damaged tissue. This study aims to describe the levels of malondialdehyde (MDA) in plasma and kidneys of type 2 diabetes mellitus rats (T2DM) with MSC-CM therapy. Twenty five male Wistar rats used in this study were divided into 5 groups: T2DM + 0.05 ml/kg BW MSC-CM (0.05); T2DM + 0.1 ml/kg BW MSC-CM (0.1); T2DM + 0.2 ml/kg BB MSC-CM (0.2); the control group with T2DM (DMT2), and healthy group (KS). Induction of T2DM by using streptozotocin-nicotinamide (STZ-NA). Treatment started on day 7 after the DM condition is reached, given 4 times with an interval of 7 days, intraperitoneally. The datas blood glucose and MDA levels will be analyzed statistically. The results showed that the induction of T2DM with STZ-NA can increase glucose and MDA levels in the blood (P <0.05). Therapy 0.05; 0.1; and 0.2 ml/kg BW MSC-CM shown to reduce blood glucose levels, plasma MDA levels and kidney (P <0.05). Levels of 0.2 ml/kg MSC-CM showed better activity in lowering blood glucose and MDA levels. Based on this research, MSC-CM can lower blood glucose levels and MDA levels in the blood and kidneys of T2DM rats.