Mesenchymal Stem Cell-conditioned Medium Promote the
Recovery of Skin Burn Wound

Padeta, Irma; Nugroho, Widagdo Sri; Kusindarta, Dwi Liliek; Fibrianto, Yuda Heru; Budipitojo, Teguh

doi:10.3923/ajava.2017.132.141

Cited by 23 publications

(27 citation statements)

References 30 publications

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“…Recent studies have suggested that the main therapeutic benefits of MSCs are not limited solely to their cell-to-cell interactions [ 36 , 37 , 38 , 39 ]. MSCs secrete a broad range of bioactive molecules, including proteins, nucleic acids, proteasomes, exosomes, microRNA and membrane vesicles, collectively known as the secretome, in response to the surrounding environment [ 40 , 41 ].…”

Section: Development Of Msc Secretome As An Alternative Cell-free mentioning

confidence: 99%

Mesenchymal Stem Cell Secretome as an Emerging Cell-Free Alternative for Improving Wound Repair

Ahangar

Mills

Cowin

2020

IJMS

123

117

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The use of mesenchymal stem cells (MSC) for the treatment of cutaneous wounds is currently of enormous interest. However, the broad translation of cell therapies into clinical use is hampered by their efficacy, safety, manufacturing and cost. MSCs release a broad repertoire of trophic factors and immunomodulatory cytokines, referred to as the MSC secretome, that has considerable potential for the treatment of cutaneous wounds as a cell-free therapy. In this review, we outline the current status of MSCs as a treatment for cutaneous wounds and introduce the potential of the MSC secretome as a cell-free alternative for wound repair. We discuss the challenges and provide insights and perspectives for the future development of the MSC secretome as well as identify its potential clinical translation into a therapeutic treatment.

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Section: Development Of Msc Secretome As An Alternative Cell-free mentioning

confidence: 99%

Mesenchymal Stem Cell Secretome as an Emerging Cell-Free Alternative for Improving Wound Repair

Ahangar

Mills

Cowin

2020

IJMS

123

117

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“…The UMSC‐CM increased the proliferation and migration of dermal fibroblasts, and the expression of TGFβ‐3 which is one of the key factors involved in scarless WH. It has also been shown that MSC‐CM enhances the wound closure, number of fibroblasts, collagen density, and numbers of blood vessels . Furthermore, other MSC‐CM factors, including TGF‐β1, IL‐6, IL‐8, fibronectin, and collagen‐I are identified as mediators of WH.…”

Section: The Role Of Mscs In Wound Healingmentioning

confidence: 97%

“…It has also been shown that MSC-CM enhances the wound closure, number of fibroblasts, collagen density, and numbers of blood vessels. 16 Furthermore, other MSC-CM factors, including TGF-β1, IL-6, IL-8, fibronectin, and collagen-I are identified as mediators of WH. Recently, researchers have focused on regeneration effects of MSCs exosomes on wound healing process.…”

Section: The Role Of Mscs In Wound Healingmentioning

confidence: 99%

A review on different methods to increase the efficiency of mesenchymal stem cell‐based wound therapy

Shojaei

Rahmati

Dehkordi

2019

Wound Repair Regeneration

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Mesenchymal stem cells (MSCs) accelerate wound healing but the harsh environment of wound site limits the engraftment, retention, and survival rate of transplanted cells. There are multiple approaches that amplify the therapeutic potential of MSCs. The MSCs derived from medical waste material, provide comparable regenerative abilities compared to traditional sources. The application of different scaffolds increases MSC delivery and migration into the wound. The spheroid culture of MSC increases the paracrine effects of the entrapped cells and the secretion of pro‐angiogenic and anti‐inflammatory cytokines. The MSC pretreating and preconditioning enhances the cell migration, proliferation, and survival rate, which lead to higher angiogenesis, re‐epithelialization, wound closure, and granulation tissue formation. Moreover, genetic modification has been performed in order to increase MSC angiogenesis, differentiation potential, as well as the cell life span. Herein, we review the results of aforementioned approaches and provide information accommodating to the continued development of MSC‐based wound therapy in the future.

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“…e CM of MSCs is the set of substances extracted from the medium in which the MSCs are cultured and contain a high number of soluble factors such as cytokines, chemokines, immunomodulatory molecules, and growth factors with potential regenerative effect [111,112]. e majority of these secreted factors found in MSC derived cell-free secretome demonstrate properties and effects identical to those described in the MSCs themselves, related to processes of intercellular communication, cell signaling, differentiation, and cell adhesion, and include elements such as extra and intracellular matrix proteins, inflammation-related cytokines, chemokines, and angiogenic factors [113].…”

Section: Immunohistochemical Analysismentioning

confidence: 99%

Rat Olfactory Mucosa Mesenchymal Stem/Stromal Cells (OM-MSCs): A Characterization Study

Alvites

Branquinho

Amorim

et al. 2020

International Journal of Cell Biology

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Stem/stromal cell-based therapies are a branch of regenerative medicine and stand as an attractive option to promote the repair of damaged or dysfunctional tissues and organs. Olfactory mucosa mesenchymal stem/stromal cells have been regarded as a promising tool in regenerative therapies because of their several favorable properties such as multipotency, high proliferation rate, helpful location, and few associated ethical issues. These cells are easily accessible in the nasal cavity of most mammals, including the rat, can be easily applied in autologous treatments, and do not cope with most of the obstacles associated with the use of other stem cells. Despite this, its application in preclinical trials and in both human and animal patients is still limited because of the small number of studies performed so far and to the nonexistence of a standard and unambiguous protocol for collection, isolation, and therapeutic application. In the present work a validation of a protocol for isolation, culture, expansion, freezing, and thawing of olfactory mucosa mesenchymal stem/stromal cells was performed, applied to the rat model, as well as a biological characterization of these cells. To investigate the therapeutic potential of OM-MSCs and their eventual safe application in preclinical trials, the main characteristics of OMSC stemness were addressed.

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Mesenchymal Stem Cell-conditioned Medium Promote the Recovery of Skin Burn Wound

Cited by 23 publications

References 30 publications

Mesenchymal Stem Cell Secretome as an Emerging Cell-Free Alternative for Improving Wound Repair

Mesenchymal Stem Cell Secretome as an Emerging Cell-Free Alternative for Improving Wound Repair

A review on different methods to increase the efficiency of mesenchymal stem cell‐based wound therapy

Rat Olfactory Mucosa Mesenchymal Stem/Stromal Cells (OM-MSCs): A Characterization Study

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