Livestock especially cattle are known as a main reservoir of Escherichia coli O157:H7. This bacterium is considered as a pathogenic agent characterized by producing toxins, which are familiarly known as Shiga-like toxin-1 (Stx1) and Stx2. The aim of this work was to analyse the novel sequence of the 16S rRNA gene of strains isolated in this study in order to know the phylogenetic relationships between these sequences and those between the sequences of bacteria available in databanks. The results of this analysis showed that the strains KL-48(2) and SM25(1) that originated from human and cattle feces, respectively, are closely related among them and with respect to E. coli EDL 933, E. coli Sakai, E. coli ATCC 43894, E. coli O111:H-, E. coli O121:H19, E. coli O104:H4, and Shigella sonnei with more than 99% similarity values.
Indonesia has many areas with the various tourism potentials but faces obstacles in the management, one of them is Suranadi Tourism Village in Narmada District, West Lombok Regency. This research aimed to analyze 1) the potential and tourism management system in Suranadi Tourism Village; 2) internal and external factors affecting the development of Suranadi Tourism Village; 3) the development strategy of Suranadi Tourism Village. This research applies some theories such as tourism destination development theory by Cooper (1993), irridex theory by Doxey (1976), and tripartite attraction design theory by Gunn (1972). This research used qualitative approach with descriptive method. Data obtained through interviews and observation. The method of data analysis in this research is by qualitative data analysis and SWOT analysis. The results showed that the potential of Suranadi Village as a cultural tourism village that is the potential of nature and culture. The various of potentials in the Suranadi Tourism Village as a whole has not managed professionally and optimally so that economic benefits are not fully felt for local community. The results of SWOT matrix analysis showed that there are four alternative strategies generated such as SO strategy (Strengths-Opportunities), ST strategy (Strengths-Threats), WO strategy (Weaknesses-Opportunities), WT strategy (Weaknesses-Threats).
Keywords: tourism potential, development strategy, Suranadi Tourism Village, SWOT
Bioactive peptides fermented milk is very potential as functional food products for health. The aim of this study is to analyze the inhibition activity of Angiotensin Converting Enzyme and kefir whey amino acid profile and IC50. The design being applied is a comprehensive random design with five treatments (fermentation time 0, 3, 6, 9 and 12 days) and 3 replications. ACE inhibition indicators include ACE inhibition with IC50, protein, peptide concentration, total proteolytic, and amino acid profiles. The results show that ACE inhibition activity ranges from 35.94 -66.67% with peptide levels of 872.80 -1084.74 mg/mL and IC50 of 65.48 µg/mL, and contained hydrophobic amino acids which functioned for ACE inhibition. The conclusion of this study that the highest ACE inhibition is obtained at 0 day of fermentation with inhibition ability (IC50) of 65.48 µg/mL and functioning as nutraceuticals food.
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