The structural and functional recovery of pancreatic β-cells in type 1 diabetes mellitus induced mesenchymal stem cell-conditioned medium

Nugroho, Widagdo Sri; Kusindarta, Dwi Liliek; Susetya, Heru; Fitriana, Ida; Mulyani, Guntari Titik; Fibrianto, Yuda Heru; Haryanto, Aris; Budipitojo, Teguh

doi:10.14202/vetworld.2016.535-539

Cited by 11 publications

(9 citation statements)

References 29 publications

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“…melatonin participation in pancreatic regeneration was attributed to its ability to scavenge free radicals as an antioxidant by stimulating messenger ribonucleic acid (mRNA) levels and activities of SOD and GPx (Maisaa and AL-Rawi, 2007;Vijayanand and Wesely, 2011;Tams, 2014and Godam et al, 2014 The present results indicated noticeable improvement of the pancreatic tissues after treatment of diabetic group with mescenchymal stem cells. These results are in agreement with Nugroho et al (2016) who reported that the structure of pancreatic tissues recovered in one week after MSCs treatment. Recent results obtained by Sato et al (2016) indicated that the sections from the pancreas after treatment with MSCs on day 28, showed apparently normal structure of both the pancreatic acini and the islets of Langerhans with their rounded and vesicular nuclei, such results confirm the present reported findings.…”

Section: Bhansali Et Al (2013)supporting

confidence: 93%

The potential role of melatonin on bone marrow mesenchymal stem cells therapy in pancreatic tissue of streptozotocin-induced diabetic rats.

Kadry

El‐Dakdoky

Rashid

et al. 2018

Journal of Scientific Research in Science

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The present study was established to investigate the role of bone marrow mesenchymal stem cells (MSCs) and melatonin (MT), alone or in combination with each other for improvement of beta cell functions in STZ-induced diabetic rat model. Also, to evaluate the role of melatonin in increasing the efficacy of MSCs. Fifty two male albino rats (130-150g) were divided into six groups. Control: received phosphate buffer saline PBS; Melatonin group received melatonin (10 mg/kg b.wt. day for 2 months by oral intubation); Diabetic untreated group; Diabetic group treated with melatonin; Diabetic group treated with mesenchymal stem cells (MSCs) (A single intravenous injection of 3×106 cell in PBS)and Diabetic group treated with both stem cells and melatonin. Diabetes was induced by a single i.p. injection of 45mg/kg b.wt. of streptozotocin. The results showed that treatment of diabetic rats with either MSCs alone or in combination with melatonin resulted in an increase in body weight and pancreas weight. The biochemical analysis showed significant improvement in glucose, insulin, total antioxidant and malondialdehyde level in diabetic rats treated with either MSCs alone or in combination with melatonin. On the other hand, the results of imumuno-histochemical analysis showed that MSCs and/or melatonin treatment reduced the rate of inflammation and apoptosis of the Islet cells of pancreases as well as increased the rate of pancreatic cell division. Such results were indicated by a significant improvement in the level of TNF-α, IL-10, PCNA, and caspase-3 to levels very close to the control. The microscopic examination of Langerhans islands showed that treatment with MSCs either alone or in combination with melatonin resulted in an improvement in the tissue of the pancreas and reduced number of damaged beta cells. It can be concluded that stem cell therapy has a significant role in restoring the structural and functional efficiency of beta cells in the pancreas. The results also showed that co-treatment of stem cells and melatonin was more effective than stem cell alone. Such result may be due to the role of melatonin as an antioxidant in increasing the efficiency and vitality of stem cells.

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Section: Bhansali Et Al (2013)supporting

confidence: 93%

The potential role of melatonin on bone marrow mesenchymal stem cells therapy in pancreatic tissue of streptozotocin-induced diabetic rats.

Kadry

El‐Dakdoky

Rashid

et al. 2018

Journal of Scientific Research in Science

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“…The secretome contained growth factors and interleukins that influenced intrapancreatic regeneration by activating and recruiting circulating stem cells and progenitor cells to injured sites, where they collaborate to repair damaged tissue. Additionally, previous research has demonstrated that MSCs circulate in injured locations and develop specifically inside tissue [ 20 ].…”

Section: Resultsmentioning

confidence: 99%

Secretome improves testosterone and androgen-binding protein production in testicular dysfunction rats induced by cisplatin

Datrianto¹,

Budipitojo²,

Prihatno³

2021

J Adv Vet Anim Res

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Objective: This study was determined to see the effects of secretome on cellular production of testosterone and androgen-binding protein (ABP) using immunohistochemistry in testicle dysfunction due to cisplatin. Materials and Methods: Forty-eight rats were divided into four groups: the healthy group, the group with testicular dysfunction, the secretome-treated group with 0.2 ml/kg body weight (BW), and the secretome-treated group with 0.3 ml/kg BW. The immunohistochemistry staining method is used to find out testosterone and ABP reactivity in tissue organs. Results: Very strong testosterone and ABP immunoreactivity were found in Leydig cells of normal testes. While in the Leydig cells of cisplatin-induced testicles, testosterone and ABP immunoreactivity were not observed. Testosterone and ABP were observed 1 week after the second secretome injection. The number of testosterone-immunoreactive cells in the low dose group from week 1 to 3 was 0, 19, and 32, respectively. From week 1 until week 4, the high dose group was 0, 29, 33, and 65, respectively. The number of ABP-immunoreactive cells from the first week until the third week in the low dose group was 0, 28, and 34, respectively. The high dose group from the first week until the fourth week was 0, 26, 58, and 83, respectively. The number of cells that produce testosterone and ABP increased from week 2 to 4. Conclusion: The administration of secretome could increase the number of immunoreactive cells toward testosterone and ABP in testicular dysfunction.

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“…Overall, the administration of MSC-CM to bone fracture cases gives good results as indicated by the accelerated regeneration of the bone. The extracts of MSCs growth media used in this study have also been reported to give good results on the regeneration of skin burns [ 30 ], skin wound [ 31 ], and beta cells of the pancreas in rats with type 1 diabetes mellitus [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Callus Formation in Fractured Femur of Rats Treated with Injection of Human Umbilical Cord Mesenchymal Stem Cell-Conditioned Medium

Anggita

Nugroho

Fibrianto

et al. 2021

Veterinary Medicine International

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Mesenchymal stem cells-conditioned medium (MSC-CM) is the extraction from stem cell medium containing biological substances, including growth factors and cytokines. These substances play roles in the various functions of body regulatory, including bone formation. However, the effect of MSC-CM derived from human umbilical cord injection in femur fracture healing of rats has not been reported previously. This study aims to see the effect of MSC-CM derived from human umbilical cord injection on the callus formation of bone fracture healing in Wistar rats (Rattus norvegicus). A femur fracture in 54 Wistar rats was made by surgery according to the procedure under sterile conditions. After the surgery, rats were divided into 2 groups of 27, respectively. Injection in the control (0.1 mL/kg body weight NaCl) and MSC-CM group (0.1 mL/kg body weight MSC-CM) was performed on weeks 0, 1, 2, 3, 4, 5, 6, 7, and 8 after surgery. Radiographic images and the femur bone samples were taken and collected on days 1, 7, 14, 21, 28, 35, and 60 after surgery. Bone samples were then fixed in Bouin solution. Histologic preparations were done by the paraffin method, by cutting the tissue blocks into 5 μm thickness and then staining with Mallory aniline blue staining. The results were analyzed descriptively and quantitatively. The result showed that the soft callus formation occurred rapidly and got wider in the MSC-CM group than that of the control group. The administration of MSC-CM injection postfracture surgery to femur fracture cases in rats was capable to accelerate the callus formation.

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The structural and functional recovery of pancreatic β-cells in type 1 diabetes mellitus induced mesenchymal stem cell-conditioned medium

Cited by 11 publications

References 29 publications

The potential role of melatonin on bone marrow mesenchymal stem cells therapy in pancreatic tissue of streptozotocin-induced diabetic rats.

The potential role of melatonin on bone marrow mesenchymal stem cells therapy in pancreatic tissue of streptozotocin-induced diabetic rats.

Secretome improves testosterone and androgen-binding protein production in testicular dysfunction rats induced by cisplatin

Callus Formation in Fractured Femur of Rats Treated with Injection of Human Umbilical Cord Mesenchymal Stem Cell-Conditioned Medium

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