Human umbilical mesenchymal stem cells conditioned medium promote primary wound healing regeneration

Kusindarta, Dwi Liliek; Wihadmadyatami, Hevi; Fibrianto, Yuda Heru; Nugroho, Widagdo Sri; Susetya, Heru; Musana, Dewi Kania; Wijayanto, Hery; Prihatna, Surya Agus; Wahyuni, A. E. T. H.

doi:10.14202/vetworld.2016.605-610

Cited by 33 publications

(38 citation statements)

References 33 publications

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“…In this context, the beneficial impact of stem cells on skin wound healing is evident, especially for the regeneration of blood vessels [41]. Stem cells or progenitor cells seem to support this process by multiple paracrine effects especially by high levels of pro-angiogenic molecules (i.e., VEGF, HGF, bFGF, EGF, TGF-β, IGF-1) [44,45,46,47]. These effects could be demonstrated in rodent diabetic wounding models, further underscoring the significant activity of stem cells and their potential in repair-resistant chronic wounds [45,46,48,49].…”

Section: Angiogenesis In Skin Wound Healingmentioning

confidence: 99%

Skin Wound Healing: An Update on the Current Knowledge and Concepts

Sorg

Tilkorn²,

Hager³

et al. 2016

Eur Surg Res

894

691

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Background: The integrity of healthy skin plays a crucial role in maintaining physiological homeostasis of the human body. The skin is the largest organ system of the body. As such, it plays pivotal roles in the protection against mechanical forces and infections, fluid imbalance, and thermal dysregulation. At the same time, it allows for flexibility to enable joint function in some areas of the body and more rigid fixation to hinder shifting of the palm or foot sole. Many instances lead to inadequate wound healing which necessitates medical intervention. Chronic conditions such as diabetes mellitus or peripheral vascular disease can lead to impaired wound healing. Acute trauma such as degloving or large-scale thermal injuries are followed by a loss of skin organ function rendering the organism vulnerable to infections, thermal dysregulation, and fluid loss. Methods: For this update article, we have reviewed the actual literature on skin wound healing purposes focusing on the main phases of wound healing, i.e., inflammation, proliferation, epithelialization, angiogenesis, remodeling, and scarring. Results: The reader will get briefed on new insights and up-to-date concepts in skin wound healing. The macrophage as a key player in the inflammatory phase will be highlighted. During the epithelialization process, we will present the different concepts of how the wound will get closed, e.g., leapfrogging, lamellipodial crawling, shuffling, and the stem cell niche. The neovascularization represents an essential component in wound healing due to its fundamental impact from the very beginning after skin injury until the end of the wound remodeling. Here, the distinct pattern of the neovascularization process and the special new functions of the pericyte will be underscored. At the end, this update will present 3 topics of high interest in skin wound healing issues, dealing with scarring, tissue engineering, and plasma application. Conclusion: Although wound healing mechanisms and specific cell functions in wound repair have been delineated in part, many underlying pathophysiological processes are still unknown. The purpose of the following update on skin wound healing is to focus on the different phases and to brief the reader on the current knowledge and new insights. Skin wound healing is a complex process, which is dependent on many cell types and mediators interacting in a highly sophisticated temporal sequence. Although some interactions during the healing process are crucial, redundancy is high and other cells or mediators can adopt functions or signaling without major complications.

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Section: Angiogenesis In Skin Wound Healingmentioning

confidence: 99%

Skin Wound Healing: An Update on the Current Knowledge and Concepts

Sorg

Tilkorn²,

Hager³

et al. 2016

Eur Surg Res

894

691

View full text Add to dashboard Cite

show abstract

“…Stem cell transplantation becomes widely studied for therapeutic approaches in the field of regenerative medicine [5-7]. Mesenchymal stem cells (MSCs) can be isolated from a variety of organs and tissues such as bone marrow, brain, skin, hair follicle, skeletal muscle, and dental pulp [8].…”

Section: Introductionmentioning

confidence: 99%

Intravenous administration of puppy deciduous teeth stem cells in degenerative valve disease

Petchdee

Sompeewong

2016

Vet World

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Aim:The objective of this study is to investigate the improvement of heart function in dogs with chronic valvular heart disease after puppy deciduous teeth stem cells (pDSCs) administration.Materials and Methods:20 client-owned dogs with degenerative valvular heart disease underwent multiple intravenous injections of allogeneic pDSCs. Dogs were randomly assigned to two groups: (i) Control group (n=10) with standard treatment for heart failure and (ii) group with standard treatment and multiple administrations of pDSCs (n=10). Electrocardiography, complete transthoracic echocardiography, thoracic radiography, and blood pressure were recorded before and after pDSCs injections for 15, 30 and 60 days.Results:Post pDSCs injection showed measurable improvement in left ventricular ejection fraction, American College of Veterinary Internal Medicine (ACVIM) functional class significantly improved and improved quality of life scores were observed. In the control group, there were no significant enhancements in heart function or ACVIM class.Conclusions:This finding suggests that pDSCs could be a supplement for valvular heart disease treatment.

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“…To solve the problem, some manipulation on the intrinsic extracellular matrix by using traditional herb such as Ocimum sanctum extract was already done. In the in vivo and in vitro model using human brain microvascular endothelial cells (HBMECs) which mimics bloodbrain barrier, the treatment of the extract may promote the cell proliferation on the hippocampus area and HBMECs in the condition upregulation of choline acetyltransferase (ChAT) enzyme [11,12]. In addition, there is also a chance to use nanometer-sized scaffolds in the presence of other substrates such as vascular endothelial growth factor or hyaluronic acid with laminin.…”

Section: Tissue Regenerationmentioning

confidence: 99%

The Role of Extracellular Matrix in Tissue Regeneration

Kusindarta¹,

Wihadmadyatami²

2018

Tissue Regeneration

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Extracellular matrix (ECM) is an extensive molecule network composed of three major components: protein, glycosaminoglycan, and glycoconjugate. ECM components, as well as cell adhesion receptors, interact with each other forming a complex network into which cells reside in all tissues and organs. Cell surface receptors transduce signals into cells from ECM, which regulate diverse cellular functions, such as survival, growth, proliferation, migration, differentiation, and some vital role in maintaining cells homeostasis. This chapter emphasizes the complex of ECM structure to provide a better understanding of its dynamic structural and functional characterization and multipotency. In this chapter the implications of ECM in tissue remodeling are mainly discuss on the neuronal regeneration and wound healing mechanism in the presence of human umbilical mesenchymal conditioned medium (HU-MSCM).

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Human umbilical mesenchymal stem cells conditioned medium promote primary wound healing regeneration

Cited by 33 publications

References 33 publications

Skin Wound Healing: An Update on the Current Knowledge and Concepts

Skin Wound Healing: An Update on the Current Knowledge and Concepts

Intravenous administration of puppy deciduous teeth stem cells in degenerative valve disease

The Role of Extracellular Matrix in Tissue Regeneration

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