Abstract. Purba JH, Wahyuni PS, Zulkarnaen, Sasmita N, Yuniti IGD, Pandawani NP. 2020. Growth and yield response of shallot (Allium ascalonicum L. var. Tuktuk) from different source materials applied with liquid biofertilizers. Biodiversitas 21: 127-133. This research was to examine growth and yield of shallots using different sources of propagation material, namely true shallot seed (TSS) and bulbs. Soil biological fertility, which was generally low, was improved by the addition of liquid organic fertilizer. The purpose of this study a) to determine the differences in the propagation of plants from seeds and bulbs of shallot Tuktuk varieties, and b) to determine the effect of liquid biofertilizer maxigrow and rhizobacteria. The study used a one-factor randomized design. The results showed that the growth and yield of shallots propagated with bulbs were better than the origin of the seeds. The treatment of the two types of liquid biofertilizer produces tangible growth and yield, but there was no significant difference between the two kinds of liquid organic fertilizer.

Abstract. Listihani L, Ariati PEP, Yuniti IGAD, Selangga DGW. 2022. The brown planthopper (Nilaparvata lugens) attack and its genetic diversity on rice in Bali, Indonesia. Biodiversitas 23: 4696-4704. The brown planthopper (Nilaparvata lugens) is an important pest on rice crops in Indonesia. The genetic diversity of BPH isolates in western Indonesia has been extensively reported, whereas eastern Indonesia isolates have not been reported. This research aims to analyze genetic diversity and evaluate the BPH attack's intensity on Bali rice plants. The research method used was an observation of attack percentage, population dynamics, attack intensity, and genetic diversity of BPH in 9 districts in Bali (Badung, Gianyar, Klungkung, Bangli, Karangasem, Tabanan, Denpasar City, Buleleng, and Jembrana). Molecular identification was carried out on N. lugens DNA in the mtCOI fragment. BPH attacks of >50% were found in the districts of Gianyar, Bangli, Jembrana, and Badung. The BPH population was primarily found in Ciherang and IR-64 varieties of rice in the Badung Regency, with 43.67 BPH per rice hill. In general, rice varieties grown in all observation locations were susceptible to BPH, such as Ciherang, IR-64, Inpari 32, and Situbagendit. In the Ciherang and IR-64 varieties, the highest attack intensity average value reached 30%. The sequence of N. lugens isolate from Bali Jembrana showed the highest nucleotide and amino acid homology with N. lugens isolate FSD-034 from Pakistan (MK301229) biotype Y of 99.5 -99.74% and 100%, respectively. This study found N. lugens biotype Y in rice plants for the first time in Indonesia. This study reported that Rice varieties Situbagendit and Inpari 32, previously resistant to BPH, are reported as susceptible to BPH.

Abstract. Sasmita N, Purba JH, Yuniti IGAD. 2019. Adaptation of Morus alba and Morus cathayana plants in a different climate and environment conditions in Indonesia. Biodiversitas 20: 544-554. Mulberry (Morus alba L. and Morus cathayana Hemsl.) cultivation business as silkworm feed in Indonesia is increasing, considering the benefits of mulberry economically, ecologically and pharmaceutically. This study aimed to determine the mulberry plants adaptation to the ability of life and growth of mulberry cuttings. The research was conducted at three different places i.e.  BPTH Bali, Cianjur Alam Sutra Nursery (West Java) and East Kutai Farming Farm, using Randomized Block Design consisted of mulberry treatments (M. alba and M. cathayana) and three treatments i.e. using Rootone F, Using Organic solution and Control (no added. The results show different growth rates from various regions in Indonesia. Mulberry growth in West Java was the best compared to mulberry which growth in Bali and Kalimantan. The highest live percentage was observed in West Java for M. alba (95%) and M. cathayana (90%), followed by Bali M. alba (85%) and M. cathayana (80%), Kalimantan M. alba (80%) and M. cathayana (75%). The best high parameter of mulberry plant in West Java for M. alba (80cm) and M. cathayana (76 cm), in Bali for M. alba (67 cm) and M. cathayana (76cm), in Kalimantan for M. alba (58 cm) and M. cathayana (50cm). The best mulberry species based on the whole parameter is M. alba in West Java. This means that West Java climate and environmental conditions are good for mulberry cultivation.

No abstract

Citrus (Citrus nobilis L.) Propagation Through Culture In Vitro Using Seed From Infected Plants Of Citrus Vein Phloem Degeneration (CVPD) Disease. The sample was taken in Belancan Village, Kintamani District, Bangli Regency, and invitro culture was conducted in UPT. Genetics Resource and Molecular Biology Laboratory in February to June 2018. This research purposes were to get the new citrus plant from citrus seed infected of CVPD disease explants with culture in vitro and found the presence of the bacterium Liberobacter asiaticus in results of citrus plants culture in vitro. The sampling method is using plants appearance. Obtained sample 1 is a plant that shows severe symptoms of CVPD disease and sample 2 plants that show no symptoms of CVPD disease. Seed Sample is cultured with MS0 medium for 8 WAP then proceed to acclimatization. The results of this research showed that the seed explants from both samples were able to grow well and did not have the characteristic of CVPD disease, but explant seed sample 2 in vitro culture result showed that the bacterial DNA bacterium Liberobacter asiaticus pathogen CVPD disease does exist in length 1160bp, so it cannot be expected to produce CVPD-free crops.

Environment quality decrease is one of the impact of coal mining activities in East Kalimantan. The land becomes barren and critical, making it difficult for plants to grow and adapt, therefore post-mining area needs to be rehabilitated to restore the soil of the land as an important factor in crop cultivation. Before being planted with local trees, the environmental conditions must be suitable for seedlings to be planted. One of the pioneer plants functions is to stabilize the post-mining environmental conditions. The objective of this study was to determine a pioneer species that have a well adapt to the post-coal mining environment in East Kalimantan. This study using completely randomized block design. The plot used a rectangular 30×25 meter plot of 15 plots for five pioneer tree species. Data collected were the plant life percentage, plant height, stem diameter and crown diameter. The study result showed that from the five species of pioneer plants there were three species has an optimal growth they are Alstonia scholaris, Homalanthus populneus and Croton argyratus. Based on the List Significance Difference test 5% level results shows the Alstonia scholaris and Croton argyratus life percentage is not significantly different with Homalanthus populneus, but it is very different from Macaranga gigantea and Trema tomentosa. Homalanthus populneus is the most adaptable pioneer species in the open area, its growing in the open post-mining area and from the forest with has intact condition. The genetic factors really affect the adaptation and growth of pioneer plants in post-mining areas.

The female workforce has productivity that plays a role and has the potential to support increased household income in rural areas. To provide guidance for women's productivity, a forum in the form of a PKK is needed that can be used by women. The purpose of this study was to identify the internal and external conditions of the PKK, as well as to determine the strategies that the PKK could use in Tabanan City in developing creative economic activities to increase member income. This research was conducted in Tabanan Regency at the PKK of each Village which was carried out from April to August 2020. Respondents consisted of 36 respondents from the PKK and 12 respondents from Kelian Dinas Banjar from the PKK. This research is a descriptive study that uses a strategic management approach, combining SWOT and AHP analysis for the best strategic decision making. The results of the research obtained are internal conditions in the development of PKK creative economic activities in Tabanan City, where the main strength factor is a solid group institution. The main weakness factors are lack of information on getting training activities and limitations in obtaining capital. Furthermore, external conditions in the development of PKK creative economic activities in Tabanan City, where the opportunity factor is good, namely the PKK group has a good image in the village government. For a major threat factor, namely the ease of duplicating products by competitors. So that we get the best strategy that can be applied in the development of PKK creative economic activities in Tabanan City, namely increasing the workforce of distributors to increase sales, in which product marketing requires product affordability to be well accepted by consumers.

Forests, traditional gardens and green spaces play an important role in regulating the water flow of an area. Along with the high demand for land in Bali for agriculture and tourism, many forest areas have been converted into hotels and settlements. Forest conversion has caused many problems such as erosion, soil fertility decreased, flora fauna extinction, floods, drought, global warming and the disturbing watershed, especially rivers for springs. The purpose of this study is the development of watershed conservation in Bali based on traditional agroforestry. The method that used is vegetation analysis. Calculation of the erosion amount using the USLE formula. Sampling was done by ten plots. The results showed that traditional agroforestry vegetation consisted of vertical and horizontal structures. The characteristic of traditional agroforestry is that dominant plants are distributed irregularly, thus creating a miniature structure like a forest. The vertical structure consists of trees, horizontal structure filled with species of garden plants and agriculture. Trees have roots spread intensively in the soil and reduce nutrient leaching. Land cover by vegetation protects the soil and erosion. The agroforestry has a role as an act of soil and water conservation. Traditional agroforestry land cover has a relatively low C coefficient (0.05-0.25) compared to other lands. The level of erosion hazard is low and moderate. Average erosion value of 55.01 t.ha-1.yr-1. This indicates that traditional agroforestry makes the soil have a higher ability to absorb water, thereby reducing surface runoff. Likewise, organic material that improves the water content capacity. In addition, water quality can be improved through the humus filter function. During a long dry season, there is a drought due to low rainfall, but rivers and springs were able to supply water for the peoples daily needs. This condition occurs because of the tree retentions in traditional gardens. Conservation actions need to be taken, namely maintaining trees vegetation, increasing reforestation, bench terraces use, mounds and mulch use. This condition also places traditional agroforestry as a sustainable land management system.