First report of Sweet potato leaf curl virus (SPLCV) on Ipomoea batatas in Bali, Indonesia

Listihani, Listihani; Yuniti, I Gusti Ayu Diah; Lestari, Putu Fajar Kartika; Ariati, Putu Eka Pasmidi

doi:10.1007/s42360-022-00489-6

Cited by 3 publications

(5 citation statements)

References 14 publications

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“…In general, viral infection symptoms on the leaves are yellowing and curling, while fruits showed abnormalities in size, color, texture, ripeness, number of seedlings, and total productivity, and several viruses cause fruit death (Listihani et al 2019(Listihani et al , 2020(Listihani et al , 2022b). As stated by Listihani et al (2022a), SPLCV infection symptoms in young leaves are changes in leaf color into vein clearing. Based on the test result up to day 21, it was observed that Eucheuma spinosum showed the best performance in suppressing SPLCV infection in sweet potato plants into symptomless disease in young leaves (Table 2, Figure 2).…”

Section: Resultsmentioning

confidence: 96%

“…SPLCV virus source plant was obtained from previous collections of SPLCV-Tegallalang isolates from Gianyar Regency (Listihani et al 2022a). The inoculum source was multiplied on sweet potato plants in different pots to get as much virus inoculum stock.…”

Section: Inoculum Multiplicationmentioning

confidence: 99%

“…More than 20 viruses infect sweet potatoes from the genus Begomovirus, Carlavirus, Cavemovirus, Crinivirus, Cucumovirus, Enamovirus, Ipomovirus, Nepovirus, Potyvirus, Solendovirus, and Tospovirus (Cuellar et al 2015;Maina et al 2018). Several important viruses in sweet potato have been reported, such as sweet potato virus C (SPVC), sweet potato feathery mottle virus (SPFMV), sweet potato feathery mottle virus strain internal cork (SPFMV-IC), sweet potato feathery mottle virus strain russet crack (SPFMV-RC), sweet potato mild mottle virus (SPMMV), sweet potato chlorotic stunt virus (SPCSV), sweet potato virus G (SPVG), and sweet potato leaf curl virus (SPLCV) (Choi et al 2012;Clark et al 2012;Kim et al 2015;Maina et al 2018;Zhang et al 2020;Listihani and Selangga 2021;Listihani et al 2022a). SPLCV (Geminiviridae; Begomovirus) was first infect sweet potatoes in Kenya, U.S., China, Japan, Spain, Uganda, Brazil, Korea, and Tanzania (Albuquerque et al 2012;Choi et al 2012;Cho et al 2020;Wanjala et al 2020;Andreason et al 2021;Bachwenkizi et al 2022).…”

Section: Introductionmentioning

confidence: 99%

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The antiviral potential of macroalgae in suppressing Sweet potato leaf curl virus (SPLCV) infection in sweet potatoes

LISTIHANI,

YUNITI,

SAPANCA

et al. 2023

Biodiversitas

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Abstract. Listihani L, Yuniti IGAD, Sapanca PLY, Pandawani NP, Selangga DGW. 2023. The antiviral potential of macroalgae in suppressing Sweet potato leaf curl virus (SPLCV) infection in sweet potatoes. Biodiversitas 24: 4079-4086. Sweet potato leaf curl virus (SPLCV) was first found in sweet potatoes in Indonesia in 2022. Prevention of spread of virus is essential, especially by using macroalgae extract which is environmentally friendly and has antiviral activity. The aim of present research was to test the potential of sea macroalgae to suppress SPLCV infection and to analyze phytochemicals of potential macroalgae containing an antiviral substance. Macroalgae extract was sprayed on the test plants that were infected by SPLCV. The observed parameters were changes in symptoms, disease incidence and severity, virus confirmation by PCR, and phytochemical analysis. The test results up to day 21 showed that Eucheuma spinosum was found to be effective in suppressing SPLCV infection in sweet potatoes, up to symptomless infection in young leaves. E. spinosum and E. cottonii suppressed disease incidence by 80% and 40% and lower disease severity as much as 71% and 48%, while E. serra showed less ability to suppress SPLCV infection. The two macroalgae had flavonoid, saponin, and steroid content which may be the reason to suppress the viral infection. The results of PCR analysis showed that microalgal extract had the highest nucleotide and amino acid homology with Gianyar (LC586170) isolate with values of 99.7 and 100%. The macroalgae with the highest ability to suppress the virus were E. spinosum and E. cottonii. This showed that the application of macroalgae extract did not change the amino acid sequence of SPLCV isolate.

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Section: Resultsmentioning

confidence: 96%

Section: Inoculum Multiplicationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The antiviral potential of macroalgae in suppressing Sweet potato leaf curl virus (SPLCV) infection in sweet potatoes

LISTIHANI,

YUNITI,

SAPANCA

et al. 2023

Biodiversitas

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“…Dini et al (2015) showed that a high stunt disease virus incidence in the field is due to stunt viruses having the ability to cause disease faster than the tungro viruses. Disease incidence is influenced by the differences in vectors transmitting the virus semi-persistently and persistently to plants (Lu et al, 2019;Listihani et al, 2020;Listihani et al, 2022b;Listihani et al, 2022c;Selangga et al, 2023). Stunt viruses are transmitted in a persistent propagative fashion to the plants by the vector N. lugens, making the vectors highly capable of transmitting the virus and causing a high incidence of stunt disease in the field.…”

Section: Resultsmentioning

confidence: 99%

Molecular characterization of Rice ragged stunt virus and Rice grassy stunt virus on Rice in Gianyar, Bali, Indonesia

Listihani,

Wiryangga Selangga,

Yuliadhi

et al. 2024

J Trop Plant Pests Dis

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Rice stunt disease is one of the causes of rice harvest failure. It is caused by the infection of Rice ragged stunt virus (RRSV) and Rice grassy stunt virus (RGSV) infection. Information about disease severity and the molecular characteristics of stunt viruses in Indonesia is still limited. Thus, this research aimed to determine the disease severity and the genetic diversity of rice stunt viruses in Gianyar, Bali. The research method consisted of observation of incidence and disease severity in the field and virus detection by reverse transcription-polymerase chain reaction (RT-PCR) using primers specific for RRSV and RGSV. The observation of the disease incidence and severity were performed in seven districts in Gianyar Regency, Bali, namely Blahbatuh, Gianyar, Payangan, Sukawati, Tampaksiring, Tegallalang, and Ubud. Stunt disease was found in all observation sites. High stunt disease incidence (> 44%) was found in three districts: Ubud, Tampaksiring, and Payangan, while the low disease incidence rate of <10% was found in Blahbatuh and Gianyar Districts. The highest stunt disease severity occurred in Tampaksiring District (60.82%), while the lowest severity occurred in Gianyar District (18.84%). The IR-64 and Ciherang cultivars are vulnerable to rice stunt disease infection. The highest homology of RRSV and RRGV nucleotides was found with Vietnam isolates being >98% and >97%, respectively. The phylogenetic analysis showed that Indonesian isolates of RRSV and RGSV were clustered in the same group as Vietnam isolates.

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“…It is suspected that insect vectors obtained the virus from tungro-infected plants near the study location. The virus incubation period in plants was affected by the timing of symptoms (Listihani et al 2018;Listihani et al 2020;Damayanti et al 2022;Listihani et al 2022b;Pandawani et al 2022;Selangga et al 2023). According to Listihani et al (2019), Selangga et al (2019), andMalathi et al (2019), the virus incubation period in plants ranges from 6-15 days.…”

Section: Determination Of Tungro-virus Infection In Rice Plantsmentioning

confidence: 99%

The relationship between vector insect populations, natural enemies, and disease incidence of tungro virus during wet and dry seasons

HUTASOIT,

JIHAD,

LISTIHANI

et al. 2023

Biodiversitas

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Abstract. Hutasoit RT, Jihad M, Listihani L, Selangga DGW. 2023. The relationship between vector insect populations, natural enemies, and disease incidence of tungro virus during wet and dry seasons. Biodiversitas 24: 4001-4007. Tungro virus is one of the most prevalent viruses affecting rice plants. The tungro virus is frequently found in rice plantations because its green planthopper vector is always present. This study aimed to determine the relationship between the population density of green planthoppers and its natural enemies with the incidence of tungro disease during the rainy and dry seasons in Lanrang, Sidenreng Rappang, South Sulawesi. The research method employed was field monitoring of the population density of green planthoppers, natural enemies, and the incidence of tungro disease. The presence of the tungro virus was confirmed by the molecular method using RTSV and RTBV-specific primers. The results showed three types of tungro vector insects: Nephotettix virescens, Nephotettix nigropictus, and Recilia dorsalis. Nephotettix virescens was the dominant vector insect, with the highest population in March and August of 101 and 51 individuals, respectively. During the dry season, the high population of the three vector insects in August was followed by a high incidence of tungro disease in September, reaching 29.38%. Symptoms of yellow leaves have been confirmed by molecular methods, which indicated that the infection was caused by RTSV and RTBV, as evidenced by the amplification of DNA bands measuring 787 bp and 1400 bp. Data on the population of vector insects and the incidence of tungro disease indicated the importance of determining the ideal time to plant to avoid the plant's susceptible phase during the peak vector population between March and August. The dominant natural enemies found during the observations included Araneus inustus, Tetragnatha maxillosa, Agriocnemis pygmaea, and Menochilus sexmaculatus. Increasing the population of natural enemies could suppress the population of vector insects.

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First report of Sweet potato leaf curl virus (SPLCV) on Ipomoea batatas in Bali, Indonesia

Cited by 3 publications

References 14 publications

The antiviral potential of macroalgae in suppressing Sweet potato leaf curl virus (SPLCV) infection in sweet potatoes

The antiviral potential of macroalgae in suppressing Sweet potato leaf curl virus (SPLCV) infection in sweet potatoes

Molecular characterization of Rice ragged stunt virus and Rice grassy stunt virus on Rice in Gianyar, Bali, Indonesia

The relationship between vector insect populations, natural enemies, and disease incidence of tungro virus during wet and dry seasons

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