Abstract. Purba JH, Wahyuni PS, Zulkarnaen, Sasmita N, Yuniti IGD, Pandawani NP. 2020. Growth and yield response of shallot (Allium ascalonicum L. var. Tuktuk) from different source materials applied with liquid biofertilizers. Biodiversitas 21: 127-133. This research was to examine growth and yield of shallots using different sources of propagation material, namely true shallot seed (TSS) and bulbs. Soil biological fertility, which was generally low, was improved by the addition of liquid organic fertilizer. The purpose of this study a) to determine the differences in the propagation of plants from seeds and bulbs of shallot Tuktuk varieties, and b) to determine the effect of liquid biofertilizer maxigrow and rhizobacteria. The study used a one-factor randomized design. The results showed that the growth and yield of shallots propagated with bulbs were better than the origin of the seeds. The treatment of the two types of liquid biofertilizer produces tangible growth and yield, but there was no significant difference between the two kinds of liquid organic fertilizer.
Abstract. Listihani L, Ariati PEP, Yuniti IGAD, Selangga DGW. 2022. The brown planthopper (Nilaparvata lugens) attack and its genetic diversity on rice in Bali, Indonesia. Biodiversitas 23: 4696-4704. The brown planthopper (Nilaparvata lugens) is an important pest on rice crops in Indonesia. The genetic diversity of BPH isolates in western Indonesia has been extensively reported, whereas eastern Indonesia isolates have not been reported. This research aims to analyze genetic diversity and evaluate the BPH attack's intensity on Bali rice plants. The research method used was an observation of attack percentage, population dynamics, attack intensity, and genetic diversity of BPH in 9 districts in Bali (Badung, Gianyar, Klungkung, Bangli, Karangasem, Tabanan, Denpasar City, Buleleng, and Jembrana). Molecular identification was carried out on N. lugens DNA in the mtCOI fragment. BPH attacks of >50% were found in the districts of Gianyar, Bangli, Jembrana, and Badung. The BPH population was primarily found in Ciherang and IR-64 varieties of rice in the Badung Regency, with 43.67 BPH per rice hill. In general, rice varieties grown in all observation locations were susceptible to BPH, such as Ciherang, IR-64, Inpari 32, and Situbagendit. In the Ciherang and IR-64 varieties, the highest attack intensity average value reached 30%. The sequence of N. lugens isolate from Bali Jembrana showed the highest nucleotide and amino acid homology with N. lugens isolate FSD-034 from Pakistan (MK301229) biotype Y of 99.5 -99.74% and 100%, respectively. This study found N. lugens biotype Y in rice plants for the first time in Indonesia. This study reported that Rice varieties Situbagendit and Inpari 32, previously resistant to BPH, are reported as susceptible to BPH.
Abstract. Sasmita N, Purba JH, Yuniti IGAD. 2019. Adaptation of Morus alba and Morus cathayana plants in a different climate and environment conditions in Indonesia. Biodiversitas 20: 544-554. Mulberry (Morus alba L. and Morus cathayana Hemsl.) cultivation business as silkworm feed in Indonesia is increasing, considering the benefits of mulberry economically, ecologically and pharmaceutically. This study aimed to determine the mulberry plants adaptation to the ability of life and growth of mulberry cuttings. The research was conducted at three different places i.e. BPTH Bali, Cianjur Alam Sutra Nursery (West Java) and East Kutai Farming Farm, using Randomized Block Design consisted of mulberry treatments (M. alba and M. cathayana) and three treatments i.e. using Rootone F, Using Organic solution and Control (no added. The results show different growth rates from various regions in Indonesia. Mulberry growth in West Java was the best compared to mulberry which growth in Bali and Kalimantan. The highest live percentage was observed in West Java for M. alba (95%) and M. cathayana (90%), followed by Bali M. alba (85%) and M. cathayana (80%), Kalimantan M. alba (80%) and M. cathayana (75%). The best high parameter of mulberry plant in West Java for M. alba (80cm) and M. cathayana (76 cm), in Bali for M. alba (67 cm) and M. cathayana (76cm), in Kalimantan for M. alba (58 cm) and M. cathayana (50cm). The best mulberry species based on the whole parameter is M. alba in West Java. This means that West Java climate and environmental conditions are good for mulberry cultivation.
Citrus (Citrus nobilis L.) Propagation Through Culture In Vitro Using Seed From Infected Plants Of Citrus Vein Phloem Degeneration (CVPD) Disease. The sample was taken in Belancan Village, Kintamani District, Bangli Regency, and invitro culture was conducted in UPT. Genetics Resource and Molecular Biology Laboratory in February to June 2018. This research purposes were to get the new citrus plant from citrus seed infected of CVPD disease explants with culture in vitro and found the presence of the bacterium Liberobacter asiaticus in results of citrus plants culture in vitro. The sampling method is using plants appearance. Obtained sample 1 is a plant that shows severe symptoms of CVPD disease and sample 2 plants that show no symptoms of CVPD disease. Seed Sample is cultured with MS0 medium for 8 WAP then proceed to acclimatization. The results of this research showed that the seed explants from both samples were able to grow well and did not have the characteristic of CVPD disease, but explant seed sample 2 in vitro culture result showed that the bacterial DNA bacterium Liberobacter asiaticus pathogen CVPD disease does exist in length 1160bp, so it cannot be expected to produce CVPD-free crops.
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