Data from several case-control studies on the relation between the Cyclin D1 (CCND1) G870A polymorphism and oral cancer susceptibility implicated conflicting conclusions. Thus, a meta-analysis was performed to derive a more precise evaluation of the association. We searched PubMed and Embase for related studies that had been published in English and eight available studies were finally included in the meta-analysis. Odd ratios (ORs) and 95 % confidence intervals (CIs) were calculated for each study. Our meta-analysis suggested that CCND1 G870A polymorphism was not associated with oral cancer risk (OR AA vs. GG = 1.08, 95 % CI = 0.90-1.30, P heterogeneity = 0.175; OR AA + GA vs. GG = 1.02, 95 % CI = 0.91-1.14, P heterogeneity = 0.781; OR AA vs. GA + GG = 1.16, 95 % CI = 0.98-1.36, P heterogeneity = 0.107; OR A vs. G = 1.05 95 % CI = 0.96-1.15, P heterogeneity = 0.211; OR GA vs. GG = 0.94, 95 % CI = 0.82-1.08, P heterogeneity = 0.935). However, in the subgroup analysis by ethnicity, possible significance among Asian groups was indicated in two genetic models (OR AA vs. GA + GG = 1.27, 95 % CI = 1.05-1.54, P heterogeneity = 0.572; OR allele A vs. allele G = 1.11, 95 % CI = 1.00-1.24, P heterogeneity = 0.211). Taken together, the meta-analysis revealed that CCND1 G870A polymorphism might be correlated with the susceptibility of oral cancer in Asians.
The present study aimed to measure the expression of WNT1 in ameloblastoma (AB). Immunohistochemistry was used to observe changes in WNT1 expression in 80 AB samples, 10 keratocystic odontogenic tumor (KCOT) samples and 10 normal oral mucosa (NOM) samples. Western blotting and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were used to measure WNT1 protein and mRNA expression, respectively, in 30 AB samples, 5 KCOT samples, 5 NOM samples and 3 tooth germ samples. Ectopic cytoplasmic expression of WNT1 was detected in AB; 88.8% (71/80) of the samples were WNT1-positive. The western blotting results demonstrated that compared with NOM (0.57±0.05), WNT1 expression was significantly higher in AB tissue (1.74±0.36, P<0.05), whereas it was not significantly different between AB and KCOT samples (0.80±0.06, P>0.05). RT-qPCR revealed that the level of WNT1 gene expression in AB was increased 2.43-fold compared with normal mucosa, and 1.77-fold compared with tooth germ tissue. In conclusion, WNT1 protein and mRNA expression were increased in AB, and there was ectopic cytoplasmic expression. This indicates that WNT1 may serve an important role in AB occurrence and development.
The objective of the study was to discuss the construction methods, characterization and biocompatibility of silk fibroin-gelatin (SF-G) three-dimensional scaffolds which meet the requirements of bone tissue engineering scaffolds. Silk fibroin (SF) and gelatin (G) were lyophilized at different temperature such as -20 °C, -40 °C, -60 °C, -80 °C then to make the composite scaffold materials crosslinked by genipin and methanol. Then to make composite materials, in order to find out the different temperature and crosslinking agent of SF and G, the following indexes including pore size, porosity, the water absorption and determination of mechanical properties were determined. The most appropriate condition of making SF-G scaffolds was prefreezing temperature is -40 °C and cross-linked by genipin, which could meet the physicochemical requirements of bone tissue engineering.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.