Construction and Characterization of Three-Dimensional Silk Fibroin-Gelatin Scaffolds

Du, Youwei; Gao, Xiuqiu; Wang, Zhiying; Jin, Dongmei; Tong, S. Y.; Wang, Xukai

doi:10.2485/jhtb.25.269

Cited by 5 publications

(3 citation statements)

References 26 publications

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“…Based on crosslinking degree data in Figure 6A, the crosslinking density became higher with increasing the gelatin content, thereby molecular chains of proteins were restrained by crosslinking points, and thus led to stiffer and more robust nanofiber mats. Besides, it should be noted that the formation of intermolecular interaction between SFs and gelatin rendered the structural integrity of nanofiber mats and yielded the increase in mechanical properties (Zhu et al, 2015;Du et al, 2016;Selvaraj and Fathima, 2017). Moreover, according to morphological changes, higher gelatin content appeared to give rise to higher crosslinking density with resulting fiber fusion, thereby decreasing the porosity and increasing fiber entanglement leading to the strength enhancement (Simonet et al, 2014;Yin et al, 2017).…”

Section: Contact Angle and Water Uptake Capacitymentioning

confidence: 99%

Fabrication and Characterization of Electrospun Silk Fibroin/Gelatin Scaffolds Crosslinked With Glutaraldehyde Vapor

Mohammadzadehmoghadam

Dong

2019

Front. Mater.

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Bombyx mori silk fibroin (SF) /gelatin nanofiber mats with different blend ratios of 100/0, 90/10, and 70/30 were prepared by electrospinning and crosslinked with glutaraldehyde (GTA) vapor at room temperature. GTA was shown to induce the conformational transition of SFs from random coils to β sheets along with increasing nanofiber diameters with the addition of gelatin into SFs. It was found that by increasing the gelatin content, crosslinking degree was enhanced from 34% for pure SF nanofiber mats to 43% for SF/gelatin counterparts at the blend ratio of 70/30, which directly affected mechanical properties, porosity, and water uptake capacity (WUC) of prepared nanofiber mats. The addition of 10 and 30 wt% gelatin into SFs improved tensile strengths of SF/gelatin nanofiber mats by 10 and 27% along with moderate increases in Young's modulus by 12 and 27%, respectively, as opposed to plain SF counterparts. However, both porosity and WUC were found to decrease from 62 to 405% for pristine SF nanofiber mats to 47 and 232% for SF/gelatin counterparts at the blend ratio of 70/30 accordingly. To further evaluate the combined effect of GTA crosslinking and gelatin content on biological response of SF/gelatin scaffolds, the proliferation assay using 3T3 mouse fibroblast was conducted. In comparison with pure SFs, cell proliferation rate was lower for SF/gelatin constructs, which declined when the gelatin content increased. These results indicated that the adverse effect of GTA crosslinking on cell response may be ascribed to imposed changes in morphology and physiochemical properties of SF/gelatin nanofiber mats. Although crosslinking could be used to improve mechanical properties of nanofiber mats, it reduced their capacity to support the cell activity. GTA optimization is required to further modulate the physico-chemical properties of SF/gelatin nanofiber mats in order to obtain stable materials with favorable bioactive properties and promote cellular responses for tissue engineering applications.

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Section: Contact Angle and Water Uptake Capacitymentioning

confidence: 99%

Fabrication and Characterization of Electrospun Silk Fibroin/Gelatin Scaffolds Crosslinked With Glutaraldehyde Vapor

Mohammadzadehmoghadam

Dong

2019

Front. Mater.

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“…It can be mixed with natural polymers, as in the examples described above, and with synthetic polymers. In addition to biopolymers described above, the group of natural polymers with which the mixtures of silk fibroin were tested are gelatin [ 146 , 147 , 148 ], cellulose [ 149 , 150 ], agarose [ 151 ], keratin [ 152 ], elastin [ 153 ], chitin [ 154 ], heparin [ 155 ], and carrageenan [ 156 ]. They can be obtained as liquid states, films, 3D porous sponges, particles, and hydrogels, and they can be used in the broadly understood biomedical field.…”

Section: Blendingmentioning

confidence: 99%

How to Improve Physico-Chemical Properties of Silk Fibroin Materials for Biomedical Applications?—Blending and Cross-Linking of Silk Fibroin—A Review

Grabska-Zielińska

Sionkowska

2021

Materials

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This review supplies a report on fresh advances in the field of silk fibroin (SF) biopolymer and its blends with biopolymers as new biomaterials. The review also includes a subsection about silk fibroin mixtures with synthetic polymers. Silk fibroin is commonly used to receive biomaterials. However, the materials based on pure polymer present low mechanical parameters, and high enzymatic degradation rate. These properties can be problematic for tissue engineering applications. An increased interest in two- and three-component mixtures and chemically cross-linked materials has been observed due to their improved physico-chemical properties. These materials can be attractive and desirable for both academic, and, industrial attention because they expose improvements in properties required in the biomedical field. The structure, forms, methods of preparation, and some physico-chemical properties of silk fibroin are discussed in this review. Detailed examples are also given from scientific reports and practical experiments. The most common biopolymers: collagen (Coll), chitosan (CTS), alginate (AL), and hyaluronic acid (HA) are discussed as components of silk fibroin-based mixtures. Examples of binary and ternary mixtures, composites with the addition of magnetic particles, hydroxyapatite or titanium dioxide are also included and given. Additionally, the advantages and disadvantages of chemical, physical, and enzymatic cross-linking were demonstrated.

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“…Silk fibroin (SF), a kind of natural protein polymer, has become a hot research topic in the development of tissue engineering [20][21][22] and is one of the biomedical materials approved by the FDA. 23 Catto et al 24 obtained tubular matrices (inner diameter = 4.5 and 1.5 mm) by electrospinning Bombyx mori SF as a scaffold for small-diameter vascular regeneration.…”

Section: Introductionmentioning

confidence: 99%

Development of an electrospun polycaprolactone/silk scaffold for potential vascular tissue engineering applications

Liu

Chen

et al. 2020

Journal of Bioactive and Compatible Polymers

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Long-distance (⩾10 mm) arterial vascular defect injury was a massive challenge affecting human health. Compared with autologous transplantation, tissue-engineered scaffolds such as biocompatible silk fibroin (SF) scaffolds have been developed because they exhibit equivalent functional repair effects without adverse reactions. However, its mechanical strength and structural stability needed to be further improved to match the longer repair cycle of blood vessels while maintaining the original biological safety. Hence, we designed and prepared SF and hydrophobic polycaprolactone (PCL) composite microfibers by an improving electrospinning method. It was found that when the weight ratio of PCL to SF was 1: 1, a microfiber scaffold with high strength (6.16 N) and minimum degradability can be obtained. More importantly, compared with natural silk fibroin, the novel composite microfiber scaffolds can slightly inhibit cell infiltration and inflammation through co-culture with HUVECs in vitro and rabbit back transplantation in vivo. Furthermore, the fabricated scaffolds also demonstrated excellent structural stability in vivo because of the well-organized PCL doping in the structure. All these results indicated that the novel PCL/SF composite microfiber scaffolds were promising candidates for vascular tissue engineering applications.

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Construction and Characterization of Three-Dimensional Silk Fibroin-Gelatin Scaffolds

Cited by 5 publications

References 26 publications

Fabrication and Characterization of Electrospun Silk Fibroin/Gelatin Scaffolds Crosslinked With Glutaraldehyde Vapor

Fabrication and Characterization of Electrospun Silk Fibroin/Gelatin Scaffolds Crosslinked With Glutaraldehyde Vapor

How to Improve Physico-Chemical Properties of Silk Fibroin Materials for Biomedical Applications?—Blending and Cross-Linking of Silk Fibroin—A Review

Development of an electrospun polycaprolactone/silk scaffold for potential vascular tissue engineering applications

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