Biosurfaktan dari mikroorganisme mempunyai sifat fisika kimia yang stabil, tidak mencemari lingkungan, mudah terurai, dapat diperbaharui, lebih murah dan memiliki toksisitas rendah sehingga dapat digunakan dalam industri farmasi sebagai antibakteri. Penelitian ini bertujuan menguji aktivitas emulsifikasi dan karakterisasi biosurfaktan dari Bacillus cereus. Uji hemolisis dan oli spreading dilakukan sebagai penapisan awal. Produksi biosurfaktan menggunakan sistem bacth yang dishaker selama 7 hari. Supernatan diuji aktivitas biosurfaktan melalui indeks emulsifikasi dan dikarakterisasi menggunakan kromatografi lapis tipis serta pengujian kualitatif antibakteri. Bacillus cereus dapat menghidrolisis darah dan menyebarkan lapisan minyak Hasil indeks emulsifikasi pada hari ke 0, 1, 3, 5, dan 7 secara berurutan 44,83%, 48,28%, 51,72%, 46,43%, dan 51,50%. Hasil KLT yang ditandai noda merah dengan penampak ninhidrin menunjukkan golongan lipopeptida. Pengujian antibakteri menggunakan Escherichia coli dan Staphylococcus aureus menunjukkan terbentuknya zona bening di sekitar kertas cakram. Bacillus cereus dapat menghasilkan biosurfaktan golongan lipopeptida dengan indeks emulsifikasi terbesar 51.72% pada hari ketiga dan memiliki aktivitas antibakteri terhadap gram positif maupun gram negatif.
Amla plant (Phyllanthus emblica L) has been empirically used to treat diabetes. The aim of this study was to determine the most potential part of amla plant that can be used as α-glucosidase and α-amylase inhibitors. The fruit was subjected to cold extraction method, while the stem underwent soxhlet extraction process. Ethanol was used as solvent for both extracts. In-vitro inhibiton against α-glucosidase and α-amylase enzyme was measured by calculating IC 50 of water fractions of stem and fruits. Fruit fraction exhibited the highest percentage of inhibitory activity on α-amylase (IC 50 =5.68% w/v), while the strongest inhibition against α-glucosidase was shown by leaves fraction (IC 50 =0.87% w/v). In conclusion, the stem of P. emblica L was potential inhibitor of α-amylase , while the leaves was potential α-glucosidase inhibitor.
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