Isolation and identification of proteolytic bacteria from pig sludge and protease activity determination

Hamdani, Syarif; Asstiyani, N; Astriany, Dewi; Wibowo, Marlia Singgih; Ibrahim, Slamet

doi:10.1088/1755-1315/230/1/012095

Cited by 9 publications

(9 citation statements)

References 13 publications

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“…The formation of clear zones around the colonies indicates proteolytic capacity of the bacterial strains with the production of the extracellular proteases that degrade the substrate casein in the culture medium. The clear zones diameter was considered as a measure of the activity and the proteolytic index (PI) of the isolates was calculated as the next equation [29]:…”

Section: Screening Of Proteolytic Capacitymentioning

confidence: 99%

“…At the same time proteolytic capacity of the liquid cultures samples collected in different culture times containing the crude protease enzyme was also tested in solid medium. For that 20 μL of supernatants were applied in filter paper discs (Macherey-Nagel 827 ATD) in Petri dishes containing TSA supplemented with 1% of skim milk and were incubated at 30 ºC for 48 h. The formation of clear zones around the discs indicates proteolytic capacity and the PI of the isolates was calculated as described in 3.4.1 section [29]. All the assays were performed in triplicate.…”

Section: Proteolytic Activity Assessmentmentioning

confidence: 99%

“…The described qualitative assay capable to detect extracellular proteases directly in the culture medium have been used in several methods for biochemical studies and in clinical laboratory for identification of bacteria providing a simple, inexpensive, straight forward method to assess the presence of proteolytic activity of a given bacterial colony [29,41,42].…”

Section: Screening Of Proteolytic Capacity Of the Bacterial Strainsmentioning

confidence: 99%

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Biodeterioration in art: a case study of Munch's paintings

et al. 2021

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Biocolonization and biodeterioration phenomena in Cultural Heritage is presently considered a relevant issue when planning conservation strategies and preservation measures in museum collections. Artworks such as easel paintings are source of various ecological niches for microbial communities' growth due to the presence of several organic resources. Therefore, the identification of proteinaceous materials may play an important role in the evaluation of their conservation status, in the characterisation of the artistic technique, and in the definition of compatible conservation/restoration processes. Another challenge is to understand the microbiota associated to the degradative processes when developing conservation strategies in CH artworks. For this study Edvard Munch paintings belonging to Munch Museum in Oslo presenting surface alterations were analysed to increase the knowledge about the materials used by the painter and try to understand the source and the dynamics of the associated colonising microbiota, helping in devising a conservation intervention plan. Immunoenzymatic assays was carried out in microsamples allowing the detection of casein as the binder used by the artist. The high throughput sequencing approaches allowed us to explore and characterise the microbial communities that colonise these artworks. Bacterial communities found in these artworks were mainly composed by species characterised by proteolytic capacity, an important biodeteriogenic characteristic for these paintings. Simulation assays performed in paint models prepared with casein as binder display signs of degradative action promoted by the proteolytic strains isolated from the damaged areas. This approach can be useful to promote effective intervention processes in E. Munch's paintings with the same pathologies.

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Section: Screening Of Proteolytic Capacitymentioning

confidence: 99%

Section: Proteolytic Activity Assessmentmentioning

confidence: 99%

Section: Screening Of Proteolytic Capacity Of the Bacterial Strainsmentioning

confidence: 99%

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Biodeterioration in art: a case study of Munch's paintings

et al. 2021

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“…GS-P4 produced highest protease activity (0.0233U/ml) was isolated from soil samples collected from farm soil, garden soil of BIMTS college campus and oil spilled area of Burhanpur (MP), India (Rupali, 2015) [14] . Hamdani et al (2019) [15] isolated 2 potential proteolytic bacteria from 10 isolates of pig sludge.…”

Section: Results and Discussion 31 Screening Of Alkaline Proteolytic Bacteriamentioning

confidence: 99%

Novel alkaline protease of Staphylococcus cohnii N3 isolated from poultry farm soil used for silver recovery from waste X-ray film

Radhathirumalaiarasu¹,

Taj²

2021

Int. J. Appl. Res.

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Alkaline proteases are one of the most widely used as industrial enzyme recently explored for its effective degrading ability in bioremediation. In the present study alkaline protease producing bacteria was isolated from soil samples collected from waste dumped area in poultry farm. The isolate was identified as Staphylococcus cohnii N3 strain by 16s rRNA sequencing analysis. The effect of pH and temperature on alkaline protease was found that showed maximum activity at pH 9 and at temperature 60 ℃ (162.U/ml). The isolated S. cohnii N3 was further experimented for silver recovery and gelatin hydrolysis. Gelatin hydrolysis was monitored by measuring increase in turbidity in the hydrolysate. Gelatin layer was stripped completely within 20 min at 40 ˚C, pH 9. Rate of gelatin hydrolysis increased with increased in time shows complete decolorization of X-ray film proves its suitability for silver recovery.

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“…Isolat bakteri ditumbuhkan di media cawan petri dan diinkubasi pada suhu 28 o C selama 72 jam. Zona bening yang terbentuk di sekitar isolat mengindikasikan bahwa isolat dapat mendegradasi protease (Hamdani et al 2019) dan dihitung indeksnya dengan rumus:…”

Section: Identifikasi Bakteri Berdasar Sikuen 16s Rdnaunclassified

AKTIVITAS METABOLIT SEKUNDER PEMACU PERTUMBUHAN DARI BAKTERI ENDOFIT ASAL KUNYIT PUTIH (Curcuma zedoaria ROSC)

Widowati¹,

Simarmata²,

Nuriyanah³

et al. 2020

bul. littro

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Endophytic bacteria colonize the plant tissue and contribute to the host plant's growth, development, and adaptation. This study aimed to examine the endophytic bacteria diversity associated with white turmeric (Curcuma zedoaria) and determine its activity as a plant growth promoter. White turmeric plants were obtained from the Indonesian Spices and Medicinal Crop Research Institute (Balittro). The isolation of endophytic bacteria from leaves, a primary and secondary rhizome of white turmeric using Nutrient Agar (NA) medium. Identification of the isolates was conducted based on 16S rDNA. The isolates were tested for their ability as a plant growth promoter and enzyme producer. Based on the morphological characteristic, 21 isolates were obtained from the primary rhizome (47.62%), secondary rhizome (19.05%), and leaves (33.33%), respectively. The sequencing result of 16S rDNA showed that the endophytic bacteria community consisted of four phyla, β-proteobacteria, γ-proteobacteria, Flavobacteria, Firmicutes, which represented eight genera. Five isolates had several activities as a plant growth promoter, while six isolates had several enzymatic activities. Thirteen isolates had both activities, as a plant growth promoter and enzyme producer, while eight isolates only had single action. Endophytic bacteria potential as plant growth promoters can be used for supporting the cultivation of white turmeric plants.

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Isolation and identification of proteolytic bacteria from pig sludge and protease activity determination

Cited by 9 publications

References 13 publications

Biodeterioration in art: a case study of Munch's paintings

Biodeterioration in art: a case study of Munch's paintings

Novel alkaline protease of <em>Staphylococcus cohnii </em>N3 isolated from poultry farm soil used for silver recovery from waste X-ray film

AKTIVITAS METABOLIT SEKUNDER PEMACU PERTUMBUHAN DARI BAKTERI ENDOFIT ASAL KUNYIT PUTIH (Curcuma zedoaria ROSC)

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