Novel alkaline protease of <em>Staphylococcus cohnii </em>N3 isolated from poultry farm soil used for silver recovery from waste X-ray film

Abstract

Alkaline proteases are one of the most widely used as industrial enzyme recently explored for its effective degrading ability in bioremediation. In the present study alkaline protease producing bacteria was isolated from soil samples collected from waste dumped area in poultry farm. The isolate was identified as Staphylococcus cohnii N3 strain by 16s rRNA sequencing analysis. The effect of pH and temperature on alkaline protease was found that showed maximum activity at pH 9 and at temperature 60 ℃ (162.U/ml). The isolated S. cohnii N3 was further experimented for silver recovery and gelatin hydrolysis. Gelatin hydrolysis was monitored by measuring increase in turbidity in the hydrolysate. Gelatin layer was stripped completely within 20 min at 40 ˚C, pH 9. Rate of gelatin hydrolysis increased with increased in time shows complete decolorization of X-ray film proves its suitability for silver recovery.