Potensi Bacillus cereus dalam produksi biosurfaktan

Setiani, Nur Asni; Agustina, Nia; Mardiah, Irma; Hamdani, Syarif; Astriany, Dewi

doi:10.24843/jbiounud.2020.v24.i02.p09

Cited by 5 publications

(6 citation statements)

References 6 publications

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“…The mechanism that causes hemolysis by biosurfactant compounds can be in the form of normal membrane dissolution at high biosurfactant concentrations or increased membrane permeability to small solutes at low biosurfactant concentrations, resulting in osmotic lysis (Setiani et al 2020). Biosurfactants have the property of surfactants to lyse cells by inducing membrane reorganization and causing changes in cell morphology.…”

Section: Discussionmentioning

confidence: 99%

“…Biosurfactants that have an emulsification index value of >30% in the screening stage are considered positive for having significant emulsification activity (Shoeb et al 2015). The interface emulsification ability of biosurfactants is influenced by their structure, components, and amphiphilic properties (Setiani et al 2020;Durval et al 2021). Nor et al (2023) reported that the Bacillus cereus KH1 cell-free supernatant emulsified with palm oil produced the largest emulsification index of 67% compared to other oils and hydrocarbons.…”

Section: Discussionmentioning

confidence: 99%

“…Cultures were harvested after the incubation period ended by centrifugation at 4032 g for 30 minutes. The supernatant was separated from the pellet and then used for the biosurfactant activity test (Setiani et al 2020).…”

Section: Production Of Biosurfactantsmentioning

confidence: 99%

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Biosurfactant Activity of Bacillus sp. Strain LP04 Isolate and Its Antifungal Potency against Ganoderma boninense and Fusarium sp.

Maherani,

Mubarik,

Priyanto

et al. 2024

HAYATI J Biosci

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Biosurfactants are a class of amphipathic molecules that various microorganisms can produce. Biosurfactants are used as biopesticides and biocontrol agents because they have antimicrobial activity, especially as antifungal agents in several species of fungal pathogens such as Ganoderma boninense and Fusarium sp. that attack crops. This study aims to detect the biosurfactant activity of Bacillus sp. and its potential as an antifungal agent against the fungi Ganoderma boninense and Fusarium sp. Biosurfactants were produced in mineral salt medium (MSM) by harvesting cell-free supernatants. Screening of biosurfactant-producing isolates was carried out using an oil-spreading assay, a hemolysis assay, and an emulsification index. The antifungal activity of the isolates was then tested using the agar diffusion method. The LP04 isolate was closely related to Bacillus thuringiensis with a 99% similarity level. It has the potential to have biosurfactant activity, which is characterized by a positive result on the oil spreading assay test and has an emulsification index of 48.33±2.87%. The cell-free supernatants of the bacterial isolate were able to inhibit the growth of Ganoderma boninense and Fusarium sp. with growth inhibition rates of 51.11% and 56.92%, respectively.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Biosurfactant Activity of Bacillus sp. Strain LP04 Isolate and Its Antifungal Potency against Ganoderma boninense and Fusarium sp.

Maherani,

Mubarik,

Priyanto

et al. 2024

HAYATI J Biosci

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“…This indicates the presence of biosurfactant compounds which belong to the lipopeptide group. The presence of spots that are not completely round in shape indicates that the biosurfactant compound is not yet pure and is still in the form of a crude extract so further purification is needed [17].…”

Section: Thin Layer Chromatography (Tlc)mentioning

confidence: 99%

Utilization POME as growth substrate for local indigenous bacteria Bacillus sp. ALP D1 in producing biosurfactant

Damayana,

Nurhasanah,

Kiswandono

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Palm Oil Mill Effluent (POME) is a liquid palm oil waste that has beneficial value because of the nutritional content it contains. This research aim to obtain biosurfactant by using POME as a growth substrate. The methods used include biosurfactant production, extraction using acid precipitation, and characterization using TLC, FTIR, and LC-MS. The results showed that the local indigenous bacteria Bacillus sp. ALP D1 can produce biosurfactant under conditions of growth of POME 3%, NaNO3 3%, pH 6, salinity 7% with an IE24 of 69.44%, diameter of oil spreading test at 8.5 cm, and positive at drop collapse test. The extraction stage produces a brownish-yellow crude extract of 0.068 g/L. Biosurfactant characterization using TLC showed pink spots with ninhydrin reagent indicating the presence of nitrogen groups from peptide in the biosurfactant. This is supported by the results of FTIR analysis with the presence of an N-H peak from the peptide group in the absorption area 3384 cm-1. The results of the LC-MS analysis showed that the biosurfactant obtained had Rt 14.64 minutes and 332.22 m/z. Based on the results, it is suspected that the biosurfactant produced by Bacillus sp. ALP D1 is a lipopeptide.

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“…Pemberian probiotik Bacillus cereus dapat mempertahankan jumlah eritrosit pada ternak [28]. Bacillus cereus merupakan bakteri gram positif yang menguntungkan bagi inangnya (29). Bacillus cereus menghasilkan enzim protease untuk mengubah protein menjadi asam amino yang dibutuhkan dalam proses pembentukan darah (30).…”

Section: Jumlah Eritrositunclassified

Pengaruh Pemberian Fitobiotik dari Ekstrak Kulit Jengkol dan Probiotik Bacillus cereus terhadap Profil Imun Darah Itik Magelang Jantan

Sihite

Pramono²,

Afifah³

2023

JIPHO

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Improving the body immune of male magelang ducks can be done through the administration of a combination of phytobiotics and probiotics as a substitute for antibiotics. The purpose of this study was to determine the effect of the combination of phytobiotics from jengkol peel extract with the probiotic Bacillus cereus on the immune profile of male magelang ducks. This research was carried out for 8 weeks of rearing which was carried out in the poultry house of the Faculty of Agriculture, Tidar University and analysis of blood profile was carried out at the Laboratory of the Prof. Soeparwi Veterinary Hospital, Faculty of Veterinary Medicine, Gadjah Mada University. The research used 100 male Magelang ducks DOD grouped into 5 treatments, namely (P0) control water without the addition of probiotics and extracts; (P1) water with the addition of 0.5% probiotics and 0.2% jengkol peel extract; (P2) water with the addition of 1% probiotics and 0.4% jengkol peel extract; (P3) water with the addition of 1.5% probiotics and 0.6% jengkol peel extract; (P4) water with the addition of 2% probiotics and 0.8% jengkol peel extract. The data was analyzed using SPSS Version 23 software using a completely randomized design (CRD) with a unidirectional pattern, then continued with the Duncan Multiple Range Test (DMRT). The results showed that the combination of phytobiotics from jengkol peel extract with Bacillus cereus no significant effect (P>0.05) on the hemoglobin, eritrocyte count and leukocyte level of male Magelang ducks. The conclusion of this study was the combination of phytobiotics from jengkol peel extract with probiotic Bacillus cereus did not affect the hemoglobin, eritrocytes count and leukocytes level in male Magelang ducks.

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Potensi Bacillus cereus dalam produksi biosurfaktan

Cited by 5 publications

References 6 publications

Biosurfactant Activity of Bacillus sp. Strain LP04 Isolate and Its Antifungal Potency against Ganoderma boninense and Fusarium sp.

Biosurfactant Activity of Bacillus sp. Strain LP04 Isolate and Its Antifungal Potency against Ganoderma boninense and Fusarium sp.

Utilization POME as growth substrate for local indigenous bacteria Bacillus sp. ALP D1 in producing biosurfactant

Pengaruh Pemberian Fitobiotik dari Ekstrak Kulit Jengkol dan Probiotik Bacillus cereus terhadap Profil Imun Darah Itik Magelang Jantan

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