Utilization POME as growth substrate for local indigenous bacteria Bacillus sp. ALP D1 in producing biosurfactant

Abstract

Palm Oil Mill Effluent (POME) is a liquid palm oil waste that has beneficial value because of the nutritional content it contains. This research aim to obtain biosurfactant by using POME as a growth substrate. The methods used include biosurfactant production, extraction using acid precipitation, and characterization using TLC, FTIR, and LC-MS. The results showed that the local indigenous bacteria Bacillus sp. ALP D1 can produce biosurfactant under conditions of growth of POME 3%, NaNO3 3%, pH 6, salinity 7% with an IE24 of 69.44%, diameter of oil spreading test at 8.5 cm, and positive at drop collapse test. The extraction stage produces a brownish-yellow crude extract of 0.068 g/L. Biosurfactant characterization using TLC showed pink spots with ninhydrin reagent indicating the presence of nitrogen groups from peptide in the biosurfactant. This is supported by the results of FTIR analysis with the presence of an N-H peak from the peptide group in the absorption area 3384 cm-1. The results of the LC-MS analysis showed that the biosurfactant obtained had Rt 14.64 minutes and 332.22 m/z. Based on the results, it is suspected that the biosurfactant produced by Bacillus sp. ALP D1 is a lipopeptide.