Aim:This study aimed to evaluate the effects of Meniran extract (Phyllanthus niruri L.) administration on leukocyte profile of broiler chickens infected with Mycoplasma gallisepticum.Materials and Methods:Thirty broiler chickens, 21 days old were divided into five treatment groups. P0 (−): Chickens without any treatment; P0 (+), P1, P2, and P3: Chickens were infected with M. gallisepticum 108 cells/ml/animal orally, then given no treatment, Meniran extract 60%, 62.5%, and 65% orally at a dose of 1 ml/kg body weight, respectively. The treatment of Meniran extract was given for 7 days.Results:Leukocyte count with the lowest number showed in Group P0 (−) and Group P3 (p>0.05). Increased number of basophils was found in Group P0 (+), Group P1, and Group P2. The highest number of heterophils was found in Group P0 (+) and was significantly different from Group P0 to P3 (p<0.05). The same pattern was also seen in the number of lymphocytes in all treatment groups. The number of monocytes showed no significant difference between all treatment groups (p>0.05).Discussion:Increased the number of leukocytes is often observed in inflammation due to general infections, trauma, or toxicity. Shifting in the number of heterophile or lymphocytes, an increase in the number of monocytes, basophils, and eosinophils may also be associated with various infectious or inflammatory conditions. Heterophils play a role as an antibacterial defense through several effective mechanisms. When infections and inflammation occur, the heterophils will increase to phagocytosis microbe.Conclusion:It can be concluded that Meniran extract (P. niruri L.) at a dose of 65% can decrease the total number of leukocytes in broilers infected with M. gallisepticum.

Penelitian ini bertujuan untuk mengukur kualitas susu sapi segar yang disetorkan oleh peternak ke Koperasi dan kemudian dihitung jumlah bakteri Escherichia coli yang selanjutnya dibandingkan dengan SNI.  Pengambilan sampel dilakukan pada saat peternak mengantarkan susu sapi segar ke koperasi. Sampel yang diambil adalah susu yang ada di dalam milk can yang diambil dengan pipet steril dan juga karet penghisap. Sampel selanjutnya disimpan di  dalam botol steril dan dibawa dengan menggunakan coolbox dilengkapi ice gel beku, selanjutnya dilakukan pengujian di Laboratorium. Selanjutnya dilakukan penanaman pada media Lauryl Tryptose Broth (LTB), Escherichia coli Broth (ECB), Levine Eosin Methylen Blue Agar (L-EMBA) dan dilakukan uji biokimia Indole, Methyl Red, Voges Proskauer, Citrate (IMViC) selanjutnya dilakukan pendugaan jumlah bakteri dengan menggunakan Most Probable Number (MPN). Hasil penelitian didapatkan bakteri Escherichia coli yang melebihi standart SNI > 3 APM/g yaitu 5 dari 16 sampel atau 31,25%. Hasil perhitungan berdasarkan MPN, sampel 7 menghasilkan angka 3APM/g, sampel 9 menghasilkan angka 3,6 APM/g, sampel 10 menghasilkan angka 23 APM/g, sampel 13 menghasilkan angka 7,4 APM/g dan sampel 14 menghasilkan angka 3,6 APM/g.

Alkaloid test: Conducted by the method of Mayer, Wagner and Dragendorff. A sample of 3 mL was added with 5 mL of 2 M HCl, stirred and cooled at room temperature. After that, 0.5 g of NaCl was added to the already cool sample then stirred and filtered. The filtrate obtained was added with drops of 2 M HCl, then separated into 4 parts (A, B, C, D). Filtrate A was used as blank, filtrate B was added to Mayer's reagent, filtrate C was to be added to Wagner reagent, while filtrate D was used for the confirmation test. Precipitate would be formed on the addition of Mayer and Wagner reagents, when identification indicates the presence of alkaloid. Confirmation test was carried out by adding 25% ammonia to filtrate D until PH changed to 8-9. Then chloroform was added, and evaporated in water bath. Then 2M HCl was added, stirred and filtered. The filtrate was divided into 3 parts. Filtrate A was used as blank, filtrate B was tested with Mayer's reagent, while filtrate C was tested with Dragendorff's reagent. The formation of a precipitate indicated the presence of alkaloid.Tannin test: 2 grams of sample was put into a mL boiling flask, then added 350 mL of distilled water and refluxed for 3 hours. The sample was cooled and transferred into 500 ml volumetric flask, then filtered and 2 ml of the filtrate was put into a 100 ml volumetric flask. 2 ml of Folin Denis reagent and 5 ml of saturated Na 2 CO 3 were added and allowed to stand for 40 minutes then the absorbance was measured at wavelength of 725 nm.

Staphylococcus aureus merupakan salah satu bakteri yang sering menyebabkan mastitis subklinis pada Kambing Peranakan Etawah (PE) yang menimbulkan kerugian ekonomi yang cukup besar akibat turunnya produksi susu. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi Staphylococcus aureus pada susu kambing PE pederita mastitis subklinis di Kelurahan Kalipuro, Banyuwangi. Sampel yang digunakan berupa susu kambing PE yang diambil pada bulan Desember 2018 di Kelurahan Kalipuro, Banyuwangi. Metode sampling yang digunakan yaitu metode purposive sampling dimana kriteria kandang yang menggunakan bahan baku kayu dan bambu, umur kambing PE 2-3 tahun, kambing yang aktif diperah dan terbukti positif 3 dan 4 mastitis subklinis melalui uji CMT. Sampel yag didapat sebanyak 17 kemudian dianalisis laboratorik di laboratorium mikrobiologi Fakultas Kedokteran Hewan Universitas Airlangga Banyuwangi. Tahap isolasi pada media MSA dan dilanjutkan tahap identifikasi dengan menggunakan pewarnaan Gram, uji katalase, uji manitol, uji koagulase dan uji VP. Diperoleh hasil yaitu terdapat 16 (94.1%) sampel positif Staphylococcus aureus.

Chronic Respiratory Disease (CRD) is a chicken respiratory disease that attacks

This research was conducted to identify non-specific bacteria found in the cervical mucus of dairy cattle in the follicular and luteal phase. Twenty cervical mucus samples were collected from ten cows during their follicular phase and ten cows during their luteal phase. Sampling was carried out with the help of an artificial insemination gun and a plastic sheath, which were protected by a sheath cover. After insertion into cervix, the tip of the plastic sheath was cut and put into a tube prefilled with phosphate buffered saline. All samples were cultured in Blood Agar media and isolated in Eosin Methylene Blue Agar, Mac Conkey Agar, and Manitol Salt Agar. Gram staining was applied to distinguish gram-positive and gram-negative bacteria; and followed with the catalase, spore, motility, and biochemical tests to further identify bacteria. The bacteria identified were of the genus Enterobacter, Staphylococcus, Corynebacterium, Streptococcus, Escherichia, and Pasteurella. It could be concluded that non-specific bacteria of the same genus were identified in cervical mucus of dairy cows in their follicular and luteal phase, in a various of percentages.

This study aimed to examine the quality of broiler carcasses in the Banyuwangi traditional market with carcass quality examination based on SNI number 3924-2009 and tetracycline antibiotic residues test based on SNI number 7424:2008. A total of ten broiler chicken sellers as representative of almost all broiler chicken sellers in the Banyuwangi market, with each seller was taken for one carcass in this research. Carcass quality examination used a standart carcasses weight 1,0-1,3 kg. Carcass quality examination was carried out with descriptive analysis with reference to the physical characteristics of SNI (Standarisasi Nasional Indonesia). The bioassay method is used to test for tetracycline antibiotic residue. The result of the carcass quality examination showed that from the whole sample consisting of conformation, disjointed or broken bones and missing parts, discolorations, and cleanliness of broiler carcasses are good enough. The percentage quality of broiler carcasses quality I is 60%, quality II is 30% and quality III is 10%. The examination of antibiotic tetracycline residues in broiler meat showed negative results indicating that no feeds containing additional antibiotics made carcasses in the traditional Banyuwangi market safe for consumption.

Background: Brucellosis in pigs at East Java Indonesia has not only cause great economic losses due to a decrease in productivity of livestock but also are zoonotic. Infection on free brucelosis pigs were initially begun with the infected pigs both male and female, or the use of superior male pigs together. The elimination of the disease either on a group or population is considered as the most effective way to prevent the spread of the disease in pigs. Prevention efforts mainly addressed to vaccination, sanitary maintenace and government policy. The purpose of this study was to isolated and identified Brucella suis as the causative agent. Material and Methods: The survey area were the pig farm owned by breeder farmers in the area of East Java Indonesia, at Kediri, Malang, Blitar and Probolinggo district. Blood samples obtained were tested with RBT. Pigs are suspected of being infected with Brucella if the RBT was positive that characterized with agglutination in the test results. If RBT was positive, bacteriological examination will be performed, with samples of visceral foetus organ, ie liver, spleen, placenta and amniotic fluid. Isolation and identification of Brucella suis were used Brucella Broth and Brucella Agar, and if the bacteri growthwill be continued with biochemical test ie H2S, urease, citrate, catalase and oxidase test. The positive results of Brucella suis showed positive urease, catalase andoxidase,but negative for citrate and H2S. Results: RBT and bacteriolgical examination showed that 1 sample was positive Brucella suis, and 19 negative. The positive results showed positive urease, catalase and oxidase,but negative for citrate and H2S Conclusion: Based on RBT test and bacteriological examination, there was 1 positive sample of brucellla suis, that is sample coming from Kediri district.