Penelitian ini bertujuan untuk mengumpulkan data bahan pakan inkonvensional dari limbah pertanian dan perkebunan di Kabupaten Banyuwangi. Pakan merupakan aspek terpenting dalam pengelolaan peternakan. Ketersediaan pakan baik di musim hujan maupun musim kemarau harus tetap ada. Penelitian ini menggunakan metode purposive sampling sehingga sampel diambil di Kecamatan Kalibaru, Genteng, Purwoharjo, Songgon, Banyuwangi, Licin, Glagah dan Wongsorejo. Sampel bahan selanjutnya dianalisis proksimat untuk mengetahui kadar serat kasar (SK), protein kasar (PK), lemak kasar (LK), bahan ekstrak tanpa nitrogen (BETN) dan kadar abu. Hasil menunjukkan bahwa limbah jerami padi, jerami kedelai, nanas, rumput gajah, alang-alang, rumput lapang, kaliandra, turi dan lamtoro memiliki dominasi kandungan gizi yang bervariasi. Simpulan penelitian bahwa bahan limbah dapat diformulasikan sebagai pakan ternak inkonvensional.

Penelitian ini bertujuan untuk mengukur kualitas susu sapi segar yang disetorkan oleh peternak ke Koperasi dan kemudian dihitung jumlah bakteri Escherichia coli yang selanjutnya dibandingkan dengan SNI.  Pengambilan sampel dilakukan pada saat peternak mengantarkan susu sapi segar ke koperasi. Sampel yang diambil adalah susu yang ada di dalam milk can yang diambil dengan pipet steril dan juga karet penghisap. Sampel selanjutnya disimpan di  dalam botol steril dan dibawa dengan menggunakan coolbox dilengkapi ice gel beku, selanjutnya dilakukan pengujian di Laboratorium. Selanjutnya dilakukan penanaman pada media Lauryl Tryptose Broth (LTB), Escherichia coli Broth (ECB), Levine Eosin Methylen Blue Agar (L-EMBA) dan dilakukan uji biokimia Indole, Methyl Red, Voges Proskauer, Citrate (IMViC) selanjutnya dilakukan pendugaan jumlah bakteri dengan menggunakan Most Probable Number (MPN). Hasil penelitian didapatkan bakteri Escherichia coli yang melebihi standart SNI > 3 APM/g yaitu 5 dari 16 sampel atau 31,25%. Hasil perhitungan berdasarkan MPN, sampel 7 menghasilkan angka 3APM/g, sampel 9 menghasilkan angka 3,6 APM/g, sampel 10 menghasilkan angka 23 APM/g, sampel 13 menghasilkan angka 7,4 APM/g dan sampel 14 menghasilkan angka 3,6 APM/g.

Food borne disease merupakan isu bagi dunia kesehatan karena masih belum bisa dikendalikan secara tuntas. Daging merupakan salah satu agen tempat berkembangnya mikroba infeksius, cemaran kimia dan fisik yang memungkinkan timbulnya food poisoning. Penanganan masalah food borne disease melalui penatalaksanaan penyediaan pemotongan melalui Rumah Potong Hewan (RPH) menjadi salah satu kunci untuk mengendalikan bahan pangan. Kualitas daging menjadi prioritas penting dalam menjamin mutu pangan. Penelitian menggunakan kajian cross sectional study dengan metode sampling purposive. Pengujian sampel di wilayah Kecamatan Banyuwangi, Genteng dan Kalibaru menjadi subjek utama dimana terdapat kios daging yang mensuplai kebutuhan konsumen sehingga akan diketahui kualitas daging yang dipotong melalui RPH. Penelitian ini menunjukkan hasil uji organoleptis warna 37% kelainan, bau normal, tekstur konsistensi keras 0,05%, uji pH 50% diatas ambang normal, uji Eber 45% positif mengalami awal kebusukan. Semua sampel daging negative Salmonella spp dan 50% melebihi ambang batas Standart Nasional Indonesia cemaran Escherichia coli.

Background and Aim: Chicken meat can be contaminated by microorganisms anywhere in the supply chain, from farm to market, and these microorganisms can be transmitted to humans through direct contact, contact with the environment, and food consumption. The microbial contamination has a serious impact on public health. This study aimed to analyze the microbial contamination of chicken meat sampled from local markets in Surabaya, East Java, Indonesia. Materials and Methods: A total of 60 samples of fresh chicken meat obtained from 10 traditional markets (six samples per market) were examined for the presence of bacteria. Staphylococcus aureus, Salmonella spp., and Escherichia coli were identified using Gram staining, culturing, and biochemical tests. The most probable number (MPN) method was used to identify E. coli. Results: Most chicken meat samples were positive for S. aureus (58.3%), Salmonella spp. (48.3%), and E. coli (40%). The samples were considered positive for E. coli if the MPN value was higher than 1×101 CFU/g. Conclusion: High microbial contamination was found in all the chicken meat sampled from local markets in Surabaya. Such contamination can lead to foodborne diseases so, proper hygiene and sanitation standards should be followed from slaughterhouses to the end-users.

Background and Aim: Stress can cause physiological and biological disorders in the body. On the other hand, antioxidants from vitamins and minerals are effective for stress treatment. Therefore, this study aimed to evaluate the effect of the administration of Vitamins C and E on serum superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GPx), testosterone, and cortisol activity in albino rats with chronic variable stress (CVS). Materials and Methods: Twenty albino rats were randomly assigned into four treatment groups: C was administered normal saline; T1 was administered Vitamins C and E; T2 was only induced CVS; and T3 was induced CVS followed by Vitamins C and E administration. All treatments were applied for 4 weeks, respectively. Furthermore, 5 mL of blood samples were collected intracardially. Body weight data were collected for the initial and final weights. From serum samples, SOD, GPx, and CAT were measured using the enzymol method; MDA was measured using the high-performance liquid chromatography method; and testosterone and cortisol were measured using the enzyme-linked immunosorbent assay method. All variables were analyzed statistically using analysis of variance followed by the Duncan test (p<0.05). Results: Our findings showed that the T1 and T3 groups significantly decreased (p<0.001) compared to T2 in the following parameters: SOD, MDA, GPx, and cortisol. Meanwhile, CAT and testosterone levels in the T1 and T3 groups were significantly increased (p<0.001) compared to the T2 group. In addition, the weight gain in T1 and T3 groups was significantly increased (p<0.001) compared to T2 group. Conclusion: It can be concluded that the administration of Vitamins C and E had a significant effect to alleviate SOD, MDA, GPx, and cortisol and to improve the testosterone level in albino rats with CVS.