Amelioration on oxidative stress, testosterone, and cortisol levels after administration of Vitamins C and E in albino rats with chronic variable stress

Hidayatik, Nanik; Purnomo, Agus Budi; Fikri, Faisal; Purnama, Muhammad Thohawi Elziyad

doi:10.14202/vetworld.2021.137-143

“…Serum cortisol was determined using ELISA method (My-Bio-Source ® , San Diego, CA, USA). 19 Meanwhile, for total protein, albumin, globulin and calcium were determined spectrophotometrically using Biuret method (Biolabo ® , France). 20 …”

Section: Methodsmentioning

confidence: 99%

“…Serum cortisol was determined using ELISA method (My-Bio-Source ® , San Diego, CA, USA). 19 Meanwhile, for total protein, albumin, globulin and calcium were determined spectrophotometrically using Biuret method (Biolabo ® , France). 20 For hematological profile, whole blood samples were determined using the clinical chemistry analyzer (Hitachi 902 ® , Roche Diagnostics, USA).…”

Section: Blood Evaluationmentioning

confidence: 99%

Risk factors, hematological and biochemical profile associated with colic in Delman horses in Gresik, Indonesia

Purnama

¹

,

Hendrawan²,

Wicaksono³

et al. 2022

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Background: Horses are herd animals that have been domesticated in the last century. In several countries, an overview of risk factors and clinical evaluation in horses with colic has not been well‐described. This study aimed to evaluate risk factors and hematological profiles in horses associated with colic in Gresik, East Java, Indonesia. Methods: A cross-sectional study was performed during April - October 2019. A total of 115 horses were diagnosed based on physical examination, clinical symptoms, and rectal examination. A questionnaire was asked to the horse-owners to analyze the risk factors while the clinical examination was performed and blood samples were collected for pre-treatment and 14 days post-treatment. Hematological profile was evaluated from a whole blood sample. Serum cortisol, plasma epinephrine, and norepinephrine concentrations were also evaluated after separating the aliquots. Results: Of the 115 horses, 96 were diagnosed with colic. The horses with colic showed a significant association between cases with gender (p<0.021), breed (p<0.000), wheat bran feeding (p<0.015), concentrate feeding (p<0.003), anthelmintics administration (p<0.000), gastrointestinal parasites (p<0.000), dental diseases (p<0.024), previous exposure to colic (p<0.000), body condition score (p<0.000), and access to water per day (p<0.000). Based on whole blood and serum evaluation, there were ameliorated significantly on the hematological profile (p<0.01), serum cortisol (p<0.05), and plasma epinephrine (p<0.01) at 14 days post-treatment. Conclusion: This study has identified factors associated with colic in Delman horses. The study provides crucial information to investigate cases of colic and to contribute the development of healthcare strategies during treatment and clinical evaluation.

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“…Initial and final weights were also measured to evaluate weight gain. After the study period, all rats were euthanized, and blood samples were collected to obtain serum for biochemical analysis [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

Dietary polyvinyl alcohol and alginate nanofibers ameliorate hyperglycemia by reducing insulin and glucose-metabolizing enzyme levels in rats with streptozotocin-induced diabetes

Suryadiningrat

¹

,

Kurniawati

²

,

Mujiburrahman

³

et al. 2021

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Background and Aim: Dietary management and antidiabetic drugs are used as therapies for diabetic patients worldwide. Alginate nanofibers were developed as a digestible food product that provides sufficient calories. This study aimed to evaluate the effect of polyvinyl alcohol (PVA) and alginate nanofibers on weight gain, blood glucose levels, and insulin and other serum parameters in diabetic rats. Materials and Methods: A total of 24 male Wistar rats were divided into six groups: (C–) Control group, (C+) diabetic rats, (T1) diabetic rats + fasting treatment for 12 h, (T2) diabetic rats + nanofibers ad libitum, (T3) diabetic rats + metformin + nanofibers ad libitum, and (T4) diabetic rats + metformin. All groups were treated for 21 days. Weight gain was evaluated by comparing initial and final weights. Blood glucose levels were evaluated weekly. Serum parameters were also evaluated at the end of the study. All variables were analyzed statistically using analysis of variance followed by Tukey's post hoc test (p<0.05). Results: The T2, T3, and T4 groups showed a significant increase in weight compared to that of the C+ and T1 groups. The T3 group had the lowest blood glucose level of all groups at the end of the study. In the serum evaluation, the T2 and T3 groups showed a significant decrease compared to the C+ group for the following variables: Alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT), creatinine, tumor necrosis factor-alpha (TNF-α), and interleukin-1β (IL-1β). In contrast, the T2 group showed a significant decrease compared to the T3 group for aspartate aminotransferase and insulin levels. Conclusion: PVA and alginate nanofibers can modulate obesity, reduce blood glucose levels, and reduce serum levels of insulin, ALT, ALP, GGT, creatinine, TNF-α, and IL-1β in diabetic rats.

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“…Serum cortisol was determined using ELISA method (My-Bio-Source ® , San Diego, CA, USA). 18 Meanwhile, for total protein, albumin, globulin and calcium were determined spectrophotometrically using Biuret method (Biolabo ® , France). 19 For hematological profile, whole blood samples were determined using the clinical chemistry analyzer (Hitachi 902 ® , Roche Diagnostics, USA).…”

Section: Blood Evaluationmentioning

confidence: 99%

Risk factors and hematological profile associated with colic in Delman horses in Gresik, Indonesia

Purnama

¹

,

Hendrawan²,

Wicaksono³

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background: Horses are herd animals that have been domesticated in the last century. In several countries, an overview of risk factors and clinical evaluation in horses with colic has not been well‐described. This study aimed to evaluate risk factors and hematological profiles in horses associated with colic in Gresik, East Java, Indonesia. Methods: A cross-sectional study was performed during April - October 2019. A total of 115 horses were diagnosed based on physical examination, clinical symptoms, and rectal examination. A questionnaire was asked to the horse-owners to analyze the risk factors while the clinical examination was performed and blood samples were collected for pre-treatment and 14 days post-treatment. Hematological profile was evaluated from a whole blood sample. Serum cortisol, plasma epinephrine, and norepinephrine concentrations were also evaluated after separating the aliquots. Results: Of the 115 horses, 96 were diagnosed with colic. The horses with colic showed a significant association between cases with gender (p<0.021), breed (p<0.000), wheat bran feeding (p<0.015), concentrate feeding (p<0.003), anthelmintics administration (p<0.000), gastrointestinal parasites (p<0.000), dental diseases (p<0.024), previous exposure to colic (p<0.000), body condition score (p<0.000), and access to water per day (p<0.000). Based on whole blood and serum evaluation, there were ameliorated significantly on the hematological profile (p<0.01), serum cortisol (p<0.05), and plasma epinephrine (p<0.01) at 14 days post-treatment. Conclusion: This study has identified factors associated with colic in Delman horses. The study provides crucial information to investigate cases of colic and to contribute the development of healthcare strategies during treatment and clinical evaluation.

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Amelioration on oxidative stress, testosterone, and cortisol levels after administration of Vitamins C and E in albino rats with chronic variable stress

Cited by 13 publications

References 53 publications

Risk factors, hematological and biochemical profile associated with colic in Delman horses in Gresik, Indonesia

Risk factors, hematological and biochemical profile associated with colic in Delman horses in Gresik, Indonesia

Dietary polyvinyl alcohol and alginate nanofibers ameliorate hyperglycemia by reducing insulin and glucose-metabolizing enzyme levels in rats with streptozotocin-induced diabetes

Risk factors and hematological profile associated with colic in Delman horses in Gresik, Indonesia

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