Penelitian ini bertujuan untuk mengukur kualitas susu sapi segar yang disetorkan oleh peternak ke Koperasi dan kemudian dihitung jumlah bakteri Escherichia coli yang selanjutnya dibandingkan dengan SNI. Pengambilan sampel dilakukan pada saat peternak mengantarkan susu sapi segar ke koperasi. Sampel yang diambil adalah susu yang ada di dalam milk can yang diambil dengan pipet steril dan juga karet penghisap. Sampel selanjutnya disimpan di dalam botol steril dan dibawa dengan menggunakan coolbox dilengkapi ice gel beku, selanjutnya dilakukan pengujian di Laboratorium. Selanjutnya dilakukan penanaman pada media Lauryl Tryptose Broth (LTB), Escherichia coli Broth (ECB), Levine Eosin Methylen Blue Agar (L-EMBA) dan dilakukan uji biokimia Indole, Methyl Red, Voges Proskauer, Citrate (IMViC) selanjutnya dilakukan pendugaan jumlah bakteri dengan menggunakan Most Probable Number (MPN). Hasil penelitian didapatkan bakteri Escherichia coli yang melebihi standart SNI > 3 APM/g yaitu 5 dari 16 sampel atau 31,25%. Hasil perhitungan berdasarkan MPN, sampel 7 menghasilkan angka 3APM/g, sampel 9 menghasilkan angka 3,6 APM/g, sampel 10 menghasilkan angka 23 APM/g, sampel 13 menghasilkan angka 7,4 APM/g dan sampel 14 menghasilkan angka 3,6 APM/g.
Staphylococcus aureus merupakan salah satu bakteri yang sering menyebabkan mastitis subklinis pada Kambing Peranakan Etawah (PE) yang menimbulkan kerugian ekonomi yang cukup besar akibat turunnya produksi susu. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi Staphylococcus aureus pada susu kambing PE pederita mastitis subklinis di Kelurahan Kalipuro, Banyuwangi. Sampel yang digunakan berupa susu kambing PE yang diambil pada bulan Desember 2018 di Kelurahan Kalipuro, Banyuwangi. Metode sampling yang digunakan yaitu metode purposive sampling dimana kriteria kandang yang menggunakan bahan baku kayu dan bambu, umur kambing PE 2-3 tahun, kambing yang aktif diperah dan terbukti positif 3 dan 4 mastitis subklinis melalui uji CMT. Sampel yag didapat sebanyak 17 kemudian dianalisis laboratorik di laboratorium mikrobiologi Fakultas Kedokteran Hewan Universitas Airlangga Banyuwangi. Tahap isolasi pada media MSA dan dilanjutkan tahap identifikasi dengan menggunakan pewarnaan Gram, uji katalase, uji manitol, uji koagulase dan uji VP. Diperoleh hasil yaitu terdapat 16 (94.1%) sampel positif Staphylococcus aureus.
Background and Aim: Antimicrobial resistance, especially antibiotic resistance, is one of the most severe public health challenges. Antibiotic resistance occurs when bacteria avoid and fight the mechanism of action of antibiotic drugs. This study aimed to determine the resistance of Escherichia coli from the milk of Ettawa crossbreed dairy goat at Blitar Regency, East Java, Indonesia, with the antibiotics streptomycin, sulfonamides, and trimethoprim. Materials and Methods: A total of 34 milk samples of Ettawa crossbreed dairy goats were used in this study. The initial stages of this research included tests of the physical properties, isolation, and identification of E. coli. Then, the E. coli isolates were tested for antibiotic resistance using the Kirby–Bauer method. Results: The results showed that all samples were positive for E. coli. The physical properties of milk, namely, color, odor, flavor, and consistency, were normal. The results of the alcohol test showed normal acidity, and the specific gravity of goat milk met the criteria, with an average specific gravity of 1.0295 g/mL. The results of the antibiotic resistance test showed that 4 (12%) samples were resistant to streptomycin, 5 (15%) to sulfonamide, and 3% to trimethoprim. Conclusion: The prevalence of E. coli from Ettawa crossbreed dairy goats in Blitar Regency, East Java, Indonesia, was 100%. Furthermore, this E. coli isolate exhibited resistance to antibiotics streptomycin, sulfonamides, and trimethoprim. The use of antibiotics in the dairy goat industry in Indonesia should be controlled to prevent the spread of resistant E. coli from animals to humans through the food chain and prevent the emergence of multidrug-resistant E. coli.
This study aimed to examine the quality of broiler carcasses in the Banyuwangi traditional market with carcass quality examination based on SNI number 3924-2009 and tetracycline antibiotic residues test based on SNI number 7424:2008. A total of ten broiler chicken sellers as representative of almost all broiler chicken sellers in the Banyuwangi market, with each seller was taken for one carcass in this research. Carcass quality examination used a standart carcasses weight 1,0-1,3 kg. Carcass quality examination was carried out with descriptive analysis with reference to the physical characteristics of SNI (Standarisasi Nasional Indonesia). The bioassay method is used to test for tetracycline antibiotic residue. The result of the carcass quality examination showed that from the whole sample consisting of conformation, disjointed or broken bones and missing parts, discolorations, and cleanliness of broiler carcasses are good enough. The percentage quality of broiler carcasses quality I is 60%, quality II is 30% and quality III is 10%. The examination of antibiotic tetracycline residues in broiler meat showed negative results indicating that no feeds containing additional antibiotics made carcasses in the traditional Banyuwangi market safe for consumption.
AbstrakTujuan penelitian ini adalah untuk mengetahui efektivitas krim ekstrak buah naga merah (Hylocereus polyrhizus) dalam meningkatkan jumlah sel fibroblas luka bakar derajat II pada tikus putih (Rattus novegicus). Dua puluh ekor tikus putih (Rattus novegicus) jantan dibagi menjadi lima kelompok perlakuan dengan jumlah empat ulangan setiap kelompok. Semua tikus tersebut diinduksi dengan luka ba kar derajat II. Kelompok kontrol negatif (K-) diterapi dengan basis krim minyak dalam air, Kelompok kontrol positif (K+) diterapi dengan Bioplacenton ® , kelompok perlakuan diterapi dengan krim ekstrak buah naga merah (Hylocereus polyrhizus) dengan konsentrasi P1 7.5%, P2 15%, dan P3 30%. Terapi diberikan dua kali dalam sehari selama 10 hari. Hasil uji Kolmogorov-Smirnov menunjukkan data terdistribusi normal (p>0.05) dilanjutkan dengan uji Oneway ANOVA yang menunjukkan adanya perbedaan nyata dari tiap kelompok perlakuan (p<0.05) dan dilanjutkan dengan uji Post-Hoc LSD. Rata-rata jumlah fibroblas setiap kelompok perlakuan menunjukkan bahwa kelompok K-dan K+ berbeda nyata dengan kelompok P1, P2 dan P3. Jumlah sel fibroblas terbanyak didapati pada kelompok P2. Kesimpulan dari penelitian yang telah dilakukan adalah krim ekstrak buah naga merah (Hylocereus polyrhizus) dapat meningkatkan jumlah sel fibroblas luka bakar derajat II pada tikus putih (Rattus norvegicus). AbstractThis study aimed to find out the effectiveness of red dragon fruit (Hylocereus polyrhizus) extract cream in increasing amount of fibroblast cells in second degree burns in white rats (Rattus norvegicus). Twenty male rats (Rattus novegicus) divided into five treatment groups with four repetitions respectively. All of them were induced with second degree burns. Negative control (K-) group treated with oil in water cream base. Positive control (K+) treated with Bioplacenton ® . P1, P2 and P3 group treated with 7.5%, 15% and 30% red dragon fruit (Hylocereus polyrhizus) extract cream. Therapy was given twice a day for 10 days. Kolmogorov -Smirnov test showed that the data distribution is normal (p>0.05), continued with Oneway ANOVA test that showed significant different from each groups (p<0.05) and continued with Post-Hoc LSD test. Average amount of fibroblast cells from each groups showed that K-group and K+ group is significanly different from P1, P2 and P3 group. P2 group showed the highest fibroblasts amount. The conclusion of this study is red dragon fruit extract (Hylocereus polyrhizus) cream can increase the amount of fibroblast cells in second degree burns in white rat's (Rattus norvegicus) skin.
Luka eksisi adalah luka yang permukaan kulit dan lapisan bawahnya akan terpotong sampai kedalaman yang bervariasi dapat terjadi secara sengaja atau tidak sengaja. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh chitosan kulit udang terhadap kepadatan kolagen dalam proses penyembuhan luka eksisi pada tikus putih. Sebanyak dua puluh ekor tikus putih (Rattus norvegicus) jantan dibagi ke dalam lima kelompok perlakuan yaitu K- luka eksisi dengan pemberian basis salep, K+ (luka eksisi+10% povidone iodine), P1 (luka eksisi+salep chitosan kulit udang 1.5%), P2 (luka eksisi+udang salep kulit kitosan 2.5%), dan P3 (luka eksisi+kitosan kulit udang 5%). Hasil perhitungan persentase kepadatan kolagen dianalisis oleh Kruskal-Wallis yang menunjukkan perbedaan signifikan (p<0.05) dan dilanjutkan dengan uji Mann-Whitney U. Kelompok K+, K- dan P1 memiliki perbedaan signifikan dalam kepadatan kolagen pada P2, dan kelompok P3 (p<0.05). Kesimpulan dari penelitian ini kitosan berpengaruh terhadap kepadatan kolagen dengan dosis 5%.
Background and Aim: Antibiotic resistance occurs when bacteria can avoid the mechanisms of action of antibiotic drugs, resulting in a reduced antibiotic activity. This is dangerous for animals and humans because treatment of infectious diseases can take longer and may even lead to treatment failure. Bacteria in meat can be the cause of meat-borne diseases for consumers. This study aimed to determine the resistance profile of Escherichia coli from broiler meat slaughtered in several local government poultry slaughterhouses in East Java. Materials and Methods: The 122 samples studied were from the pectoralis muscle of broilers from local government poultry slaughterhouses. The isolation and identification of E. coli from broiler meat were confirmed using MacConkey agar and eosin methylene blue agar, followed by Gram-staining, and an indole methyl red, Voges–Proskauer, and citrate test. The E. coli isolates were then tested for antibiotic resistance using the Kirby–Bauer method, and the results were interpreted using Clinical and Laboratory Standards Institute guidelines. Results: The isolation and identification tests for E. coli indicated that 44.26% (54) of the samples were positive for E. coli. The results of the antibiotic resistance tests demonstrated that the resistance, from highest to lowest, was to erythromycin, trimethoprim, ampicillin, ciprofloxacin, streptomycin, cephalothin , tetracycline, and chloramphenicol antibiotics with resistance of 66.7%, 61.1%, 59.3%, 35.2%, 33.3%, 27.8 %, 24.1%, and 24.1% respectively. Of the 54 isolates, 32 (59.26%) were resistant to ≥3 antimicrobials. Conclusion: The study found that the prevalence of E. coli in broiler meat in East Java, Indonesia was 44.26%. These bacteria were resistant to all of the antibiotics that were examined with high to very high resistance levels and are associated with multi-drug resistance (MDR) (59.26%). The presence of E. coli in broiler meat for human consumption can cause meat-borne illness, and the discovery of MDR is a matter of concern in the One Health approach because apart from having an impact on human health as meat consumers, it can also have an impact on animal health and the environment. Keywords: antibiotic resistance, broiler meat, Escherichia coli, public health.
Penelitian ini bertujuan untuk mengetahui kualitas semen sapi Rambon Banyuwangi dengan menggunakan dua pengencer yang berbeda, yakni tris kuning telur dan susu skim kuning telur. Metode penelitian ini menggunakan rancangan acak lengkap (RAL). Penelitian ini menggunakan satu ekor pejantan sapi Rambon untuk penampungan semen. Semen ditampung sebanyak sembilan kali untuk dua perlakuan pengencer yaitu P1: semen+tris kuning telur dan P2: semen+susu skim kuning telur. Parameter yang diukur pada penelitian ini adalah motilitas, viabilitas dan abnormalitas spermatozoa. Setiap parameter pada masing-masing perlakuan dibandingkan dengan menggunakan uji T dua sampel. Hasil penelitian ini menunjukkan bahwa P1 lebih mampu mempertahankan motilitas dan viabilitas spermatozoa sapi Rambon Banyuwangi dibanding dengan P2, dimana motilitas P1 yaitu 67,22±4,41% dengan kecepatan 3 dan motilitas P2 yaitu 59,44±8,46% dengan kecepatan 2 berbdea nyata (p<0,05). Viabilitas P1 adalah 77,44±3,21% dan P2 yaitu 73,56±3,32% berbeda nyata (p<0,05). Tidak terdapat perbedaan yang nyata (p>0,05) terhadap abnormalitas spermatozoa baik primer maupun sekunder sapi Rambon Banyuwangi pada kedua pengencer dimana nilai abnormalitas primer P1 yaitu 0,33±0,50% dan P2 0,22±0,44%, abnormalitas sekunder P1 yaitu 8,33±2,17% dan P2 7,56±2,24%. Disimpulkan bahwa media pengencer tris kuing telur dan susu skim kuning telur layak untuk digunakan IB dengan semen segar.
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