The study aim was analyzed the potential of semi polar compounds from lemon-local on old mice lipid profile with high fat dietary. Experimental study using DDY mice, males, 50−55 weeks. Five of 30 mice were sacrificed before treatment (group 6), group 1 (negative control), groups 2, 3, 4 and 5 were fed high fat dietary, group 3, 4 and 5 were treated doses 1, 2 and 3, respectively, orally. Parameters measured were body weight, HDL, TAG, total cholesterol. The results showed body weight of mice were above normal and dose 2 treatment cause weight loss (p=0.658). The highest cholesterol level was in positive control and the lowest was in the group given doses 2 (p=0.042). The highest triglycerides level was in the pretreatment group and the lowest was in the group given doses 2 (p=0.148). The highest HDL was in the positive group and the lowest was in the group given doses 2 (p=0.278). Semi polar compound from lemon-local at dose 2 could be regulated profile lipid on old mice with high fat dietary. This research needs to be improved, especially biomechanism of active substances.
Dislipidemia merupakan salah satu faktor risiko dari non alcoholic fatty liver disease (NAFLD). NAFLD mempunyai karakteristik steatosis hepatik, hepatocyte ballooning, inflamasi lobular, dan fibrosis. Kandungan flavonoid pada Citrus limon dipercaya dapat mencegah steatosis hepatik. Tujuan penelitian ini mengetahui pengaruh fraksi air buah lemon terhadap gambaran morfologi jaringan hati mencit tua yang diberi pakan tinggi lemak. Penelitian ini merupakan penelitian eksperimental dengan subjek penelitian adalah mencit (Mus musculus) jantan galur DDY tua yang dibagi menjadi empat kelompok secara acak, terdiri atas kelompok kontrol dan tiga kelompok perlakuan dengan konsentrasi fraksi air buah lemon 20,6; 41,2; 82,4 mg/20 gram BB mencit. Data jumlah hepatosit dengan droplet lemak dan hepatocyte ballooning dianalisis menggunakan uji ANOVA dan Uji Kruskal Willis. Terdapat perbedaan jumlah hepatosit dengan droplet lemak (p=0,063) dan hepatosit yang mengalami pembengkakan (p=0,109) antara kelompok kontrol dan kelompok perlakuan. Simpulan penelitian ini adalah fraksi air buah lemon dapat mencegah hepatocyte ballooning dan pembentukan droplet lemak pada hepatosit mencit tua yang diberikan pakan tinggi lemak. PROTECTIVE EFFECT OF WATER FRACTION OF LEMON ON HIGH-FAT DIET-INDUCED LIVER INJURY IN OLD MICEDyslipidemia is one of the risk factors of non alcoholic fatty liver disease (NAFLD). NAFLD is characterized by hepatic steatosis, hepatocyte ballooning, lobular inflammation, and fibrosis. Flavonoid in Citrus limon is believed to prevent hepatic steatosis. The aim of this study is to know the protective effect of lemon’s water fraction on high-fat diet-induced liver injury in old mice. This was an experimental study with old male mice (Mus musculus) DDY strain divided into four groups randomly, consisting of control group and three groups given with water fraction of lemon at concentration 20.6; 41.2; 82.4 mg/20 gram mice body weight. Total count of hepatocytes with fat droplets and hepatocytes ballooning were analyzed using ANOVA and Kruskal Willis tests. There are differences in the amount of hepatocytes with fat droplets (p=0.063) and hepatocytes ballooning (p=0.109) between the control group and the treatment group. The conclusion of this study is lemon’s water fraction can prevent the formation of hepatocyte ballooning and fat droplet in old mice’s hepatocyte fed by high-fat diet.
Background: Infertility can be caused by prolonged use of paracetamol that leads to a decrease in the sperm concentration, motility, and morphology. Dates (Phoenix Dactilyfera L) have natural antioxidant effects through several mechanisms, such as neutralizing free radicals, against NO, OH, and H2O2, and also preventing lipid peroxidation. This study aimed to investigate the effect of dates on sperm concentration, motility, and morphology. Methods: An experimental study was conducted in the
Salah satu upaya mengurangi peningkatan penduduk, yaitu dengan menggunakan kontrasepsi. Namun penggunaan kontrasepsi pria masih minim sehingga diperlukan upaya untuk meningkatkan penggunaan kontrasepsi pria. Buah pare merupakan tanaman tradisional yang dapat digunakan sebagai bahan kontrasepsi karena mengandung kukurbitasin. Tujuan penelitian ini adalah mengetahui pengaruh buah pare terhadap ketebalan tubulus seminiferus pada mencit jantan sehingga memberikan efek infertil. Penelitian dilakukan di Laboratorium Biologi Medik Fakultas Kedokteran Universitas Padjadjaran pada 17 Mei sampai 20 Juni 2018. Pengukuran ketebalan tubulus seminiferus dimulai dari spermatogonia pada lapisan basal sampai dengan kepala spermatid pada distal lumen. Subjek penelitian yang digunakan adalah 28 ekor mencit jantan yang dibagi menjadi empat kelompok; kelompok kontrol negatif, perlakuan 1 (P1) yang diberi dosis 280 mg/kgBB/hari, perlakuan 2 (P2) yang diberi dosis 560 mg/kgBB/hari dan perlakuan 3 (P3) yang diberikan dosis 1.120 mg/kgBB/hari. Ketebalan tubulus seminiferus normal pada mencit adalah 54-62 mm. Dari hasil uji hipotesis one way ANOVA, pemberian ekstrak etanol pare menurunkan ketebalan tubulus seminiferus secara keseluruhan, dengan ketebalan mencapai 39,56 mm pada dosis optimal 1.120 mg/kgBB/hari. Zat aktif kukurbitasin mempunyai struktur mirip dengan steroid sehingga dapat menurunkan kadar testosteron dan memengaruhi spermatogenesis. Sel spermatogenik yang menurun menyebabkan penurunan ketebalan tubulus seminferus.
Diabetes melitus merupakan salah satu penyakit yang menjadi masalah terbesar pada abad 21. Obesitas (terutama obesitas viseral) dan resistensi insulin sering disertai dengan sekelompok kelainan yang disebut sindrom metabolik yang mencakup intoleransi glukosa, trigliserida tinggi, kolesterol HDL rendah, dan hipertensi. Lemon mengandung flavonoid yang dipercaya mempunyai aktivitas menurunkan kadar glukosa darah. Tujuan penelitian ini mengetahui perubahan kadar glukosa darah pada mencit tua yang diberi pakan tinggi lemak setelah pemberian fraksi air buah lemon (Citrus limon). Penelitian dilakukan di Laboratorium Hewan Gedung Farmasi ITB dan Laboratorium Hewan Fakultas Kedokteran Universitas Islam Bandung pada bulan April−Juni 2018. Metode penelitian ini adalah eksperimental laboratorium murni in vivo dengan desain penelitian rancangan acak lengkap terhadap 28 mencit tua jantan galur DDY yang terbagi dalam lima kelompok, yakni kontrol normal, kontrol negatif, konsentrasi 20,6 mg/20 gBB, 41,2 mg/20 gBB, dan 82,4 mg/20 gBB. Pengukuran glukosa darah puasa dilakukan setelah masa adaptasi, saat perlakuan (hari ke-15), dan setelah perlakuan menggunakan glukosameter. Analisis data menggunakan Uji Kruskall-Wallis dan Uji Friedman. Hasil penelitian menunjukkan perbedaan median GDP1 antarkelompok yang signifikan (p=0,05), perbedaan median GDP2 yang tidak signifikan (p=0,08), dan perbedaan median GDP3 yang tidak signifikan (p=0,66). Terdapat perbedaan median GDP1−3 yang signifikan antara kelompok konsentrasi fraksi air buah lemon (p=0,04). Simpulan, fraksi air buah lemon memiliki efek menurunkan glukosa darah.EFFECT OF WATER FRACTION OF LEMON (CITRUS LIMON) ON BLOOD GLUCOSE LEVEL OF OLD MICE GIVEN HIGH-FAT DIETDiabetes mellitus is one of the biggest problems of the 21st century. Obesity (especially visceral obesity) and insulin resistance often present with a group of disorders commonly called metabolic syndrome including glucose intolerance, high triglycerides, low HDL cholesterol, and hypertension. Flavonoid compounds in lemon is believed to have blood glucose lowering activity. The purpose of this study was to determine changes in blood glucose level in old mice given a high-fat diet after administration of water fraction of lemon (Citrus limon). This study was held at Animal Laboratory of Pharmacy ITB and Animal Laboratory of Faculty of Medicine Bandung Islamic University in April to June 2018. The method of this study was pure in vivo laboratory experiment with a completely randomized design to 28 old male DDY strain mices divided into five groups; normal control, negative control, concentration 20.6 mg/20 gBW, 41.2 mg/20 gBW, and 82.4 mg/20 gBW. Fasting blood glucose measurements were performed after adaptation, ongoing treatment (day 15), and after treatment using glucosemeter. Data analysis used Kruskall-Wallis test and Friedman test. The results showed that there was a significant GDP1 median difference of each groups (p=0.05), a nonsignificant GDP2 median difference (p=0.08), and GDP3 median difference were not significantly different (p=0.66). There was a statistically significant difference between median GDP1−3 between each water fraction of lemon concentration groups (p=0.04). Conclusion, the water fraction of lemon has the effect of lowering blood glucose.
Abstract.Hypertension is a fatal non-communicable disease worldwide. The still-controversial risk factor for high blood pressure is the habit of drinking coffee. The aim of this study was to determine the relationship between coffee drinking habits and hypertension in the 18-40 age group at UPT Puskesmas Pasirkaliki, Bandung city. The method used in this study is a case-control method with non-probability sampling technique, namely purposeful sampling. This study was conducted from November 2021 to September 2022. A total of 162 respondents participated in this study. Data collection was done in two ways, secondary data of the patient and primary data from the patient questionnaire. Data processing by Chi-Square test. The results showed that 49 subjects had hypertension with the highest age of hypertension in the age group from 26 to 40 years old, with up to 40 respondents. There was no association between coffee drinking habits and hypertension with a p-value of 0.157 (p>0.05). The rate of hypertension is not only due tothe habit of drinking coffee but can be caused by other factors such as stress, smoking, unhealthy eating habits. Abstrak. Hipertensi merupakan penyakit tidak menular yang menyebabkan kematian di seluruh dunia. Faktor risiko hipertensi yang masih menjadi perdebatan adalah kebiasaan konsumsi kopi. Tujuan dari penelitian ini untuk mengetahui hubungan kebiasaan konsumsi kopi terhadap hipertensi pada rentang usia 18 – 40 tahun di UPT Puskesmas Pasirkaliki Kota Bandung. Metode yang digunakan dalam penelitian ini adalah metode case control dengan teknik pengambilan sampel non-probability sampling, yaitu purposive sampling. Penelitian ini dilaksanakan November tahun 2021 sampai bulan September tahun 2022. Sejumlah 162 responden ikut serta dalam penelitian ini. Pengumpulan data dilakukan dengan dua cara, data sekunder pasien dan data primer dari kuesioner yang diberikan kepada pasien. Pengolahan data menggunakan uji Chi-Square. Hasil penelitian menunjukkan 49 subjek mengalami hipertensi dengan rentang usia terbanyak mengalami hipertensi pada usia 26 – 40 tahun sebanyak 40 responden. Tidak terdapat hubungan antara kebiasaan konsumsi kopi dengan hipertensi dengan p-value sebesar 0,157 (p>0,05). Kejadian hipertensi tidak hanya diakibatkan karena kebiasaan konsumsi kopi, tetapi dapat disebabkan oleh faktor lain seperti stres, kebiasaan merokok, dan pola makan yang tidak sehat.
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