Bifidobacteria belongs to the so-called beneficial intestinal flora. Before attempting to raise their intestinal levels to improve the health status of the host, it is importance to know about physiological variations in the Bifidobacterial colonization of the human intestine. Birth process influenced the diversity of Bifidobacteria in infant feces. This research was intended to isolate and characterize Bifidobacterium spp. as well to evaluate their presence in the feces of infants who were born by mode of normal, caesarean and premature. The research was conducted by survey method and data were analyzed with descriptive analysis. Bifidobacterium character was observed include colony and cell morphology. The biochemical test included catalase, oxidase, indole, Voges-Proskauer, different pH growth, and resistance to lysozyme. Bifidobacterium metabolites obtained tested its bacterial activity to Salmonella typhimurium and Escherichia coli. The result of this research showed that 35 isolates are suspected Bifidobacterium group and after API 20 A test showed 17 isolates are rally genera of Bifidobacterium spp.and all isolates come from infant feces with caesar and premature delivery. These isolates inhibited the growth of S. typhimurium and E. coli with different inhibitory capabilities. This finding is very important for science and medical point of view and could be developed with further research.
Baron beach is a specific habitat due its characteristic as a narrow beach with outlet of sub-surface rivers. This researchhad been done in order to know its microbial characteristic. Research used survey method and bacterial identification wasdone based on comparative description. The research showed that during high tide of seawater, the total bacterial countwas 1.0 to 6.0 x 107 cfu/ml on NA and 2.0 to 7.0 x 107 cfu/ml on NA+0.5% NaCl. Furthermore, during the low tide of seawater, the total bacterial count was 1.4 to 8.8 x 108 cfu/ml on NA, and 3.2 to 9.0 106 cfu/ml on NA+0,5% NaCl. This studyfound 14 genera of bacteria and dominated by Gram-negative bacteria. The result indicated seawater influenced thenumber of bacteria present in this environment.© 2003 Jurusan Biologi FMIPA UNS SurakartaKey words: bacteria, aerobic-heterotrophic, Baron
Lactic acid bacteria (LAB) are used in the making of soyghurt such Lactobacillus bulgaricus, Streptococcus thermophillus, and Bifidobacterium sp. with optimum growth temperature at 36° -37° C and the incubation time ranges from 1-2 days. LAB is known able to produce bacteriocins that can inhibit pathogenic bacteria in the digestive tract such as Bacillus cereus. The purpose of this study was to determine the ratio of LAB on soyghurt on inhibition of B. cereus, determine the length of incubation soyghurt with the addition of Bifidobacterium sp. on the growth of B. cereus, and determine the effect of LAB concentrations and longer incubation at soyghurt on the growth of B. cereus. The results show a comparison of LAB (L. bulgaricus : S. thermophillus : Bifidobacterium sp.) 1: 1: 1 (K1) on soyghurt able to inhibit B. cereus. Long incubation soyghurt with the addition of Bifidobacterium sp. optimal at 24 hour on the growth of B. cereus. LAB concentration with a long incubation at soyghurt not able to increase the growth inhibitory B.cereus.
Nasopharyngeal carcinoma (NPC) is a malignant tumor that grows in the nasopharynx with a predilection in the fossa Rosenmuller. Epithelial malignancies are often found in populations of China and Southeast Asia, including Indonesia. The NPC incidence in year 2008 as many as 84,400 cases and 51,600 of these cases resulted in death. A total of 120 new cases per year NPC found in hospitals Prof. dr. Margono Soekarjo (RSMS), Purwokerto. The NPC is difficult to be diagnose caused its primary tumor lies closed to the skull base as well as various structures of vital organs. Therefore, methods that can detect early NPC required for inspection.The etiology of NPC is multifactorial consisting of genetic factors, factors of infection Epstein-Barr Virus (EBV) and environmental factors.EBV has two phases in the cycle of infection that is the phase of lytic and latent phase. BRLF1 has an important function as mediator transition from latent e NPC. The research aimed to analysis mRNA BRLF1 expression as a biomarker of NPC diagnosis by RT-PCR and to determine the positivity of RT-PCR method to detect the expression of mRNA BRLF1. The research design was cross sectional study. Samples were FFPE tumor biopsy of NPC WHO III and the total samples were 22 individu from Department of Pathology Anatomy, Prof. Dr. Margono SoekarjoHospital, Purwokerto with informed consent. The positivity of mRNA BRLF1 from FFPE tumor biopsy of NPC WHO III was in 63.6%indicating a high expression.. Keywords: mRNA BRLF1, Epstein-Barr Virus, FFPE, Nasopharyngeal Carcinoma AbstrakKarsinoma Nasofaring (KNF) adalah tumor ganas yang tumbuh di nasofaring dengan predileksi pada fosa Rosenmuller. Keganasan epitel sering ditemukan pada populasi Cina dan Asia Tenggara, termasuk Indonesia. Kejadian KNF di tahun 2008 sebanyak 84.400 kasus dan 51.600 kasus ini mengakibatkan kematian. Sebanyak 120 kasus baru per tahun KNF ditemukan di RSUD dr. Dr. Margono Soekarjo (RSMS), Purwokerto. KNF sulit untuk didiagnosis karena tumor utamanya terletak dekat dengan dasar tengkorak serta berbagai struktur organ vital. Oleh karena itu, metode yang dapat mendeteksi KNF awal diperlukan untuk pemeriksaan. Etiologi KNF bersifat multifaktorial yang terdiri dari faktor genetik, faktor infeksi Virus Epstein-Barr (EBV) dan faktor lingkungan. EBV memiliki dua fase dalam siklus infeksi yaitu fase fase litik dan laten. BRLF1 memiliki fungsi penting sebagai mediator transisi dari laten ke fase litik. Ekspresi mRNA BRLF1 yang dilakukan dengan metode RT-PCR dapat digunakan untuk mendukung diagnosis KNF, sehingga metode ini dapat meningkatkan efisiensi pemeriksaan KNF. Penelitian ini bertujuan untuk menganalisis ekspresi mRNA BRLF1 sebagai biomarka diagnosis KNF dengan teknik RT-PCR dan untuk mengetahui sensitivitas metode RT-PCR untuk mendeteksi ekspresi mRNA BRLF1. Desain penelitiannya adalah penelitian cross sectional. Sampel adalah biopsi tumor FFPE dari pasien KNF WHO III dan total sampel adalah 22 individu pada Departmen Patologi Anatomi, Rumah Sakit Prof. Dr. Margono Soekarjo, Pu...
This research aimed to identify LMP-1 EBV gene as a biomarker in NPC diagnosis. Cross-sectional study with consecutive sampling technique was applied. Samples were the whole blood of NPC WHO-3 patients collected from those untreated since 2014 in the Department of Otorhinolaryngologica, Prof. dr. Margono Soekarjo Hospital, Purwokerto. A total of 22 research subjects for NPC WHO-3 patients were completed with informed consent. The samples were subjected to DNA isolation by using Purelink® DNA/RNA viral (Invitrogen) kit protocols to obtain 100µL DNA solution, which were then stored for a long period of time at -80°C. Conventional PCR technique was performed to detect LMP1 gene to amplify the DNA of LMP1 gene resulting in a DNA amplicon of 142 bp. Analysis of the PCR method sensitivity and the results of LMP-1 gene identification were carried out descriptively by comparing the detection results obtained in this study with the results of previous ones related. The sensitivity of LMP1 gene is 77.27%, indicating a high sensitvity.The results show that LMP-1 gene as a biomarker in the diagnosis of NPC can be detected using conventional PCR technique. Conventional PCR sensitivity in the detection of LMP1gene shows higher sensitivity in compare to LMP-1 EBV gene deletion of 30 bp and LMP2A. Keywords: Epstein-Barr Virus, LMP-1 gene, Diagnosis, Nasopharyngeal Carcinoma AbstrakPenelitian ini bertujuan untuk mengidentifikasi gen LMP-1 EBV sebagai biomarka diagnosis KNF. Desain penelitian ini adalah studi cross-sectional dengan teknik consecutive sampling. Sampel adalah darah total pasien KNF WHO-3 yang dikumpulkan dari pasien yang belum menjalani terapi dari tahun 2014 pada Departemen Telinga Hidung Tenggorok -Kepala Leher, Rumah Sakit Prof. dr. Margono Soekarjo, Purwokerto. Total subyek penelitian adalah 22 orang untuk NPC WHO-3 pasien dengan informed consent. Sampel diisolasi dengan protokol kit Purelink® DNA / RNA (Invitrogen) untuk mendapatkan larutan DNA 100μL dan disimpan dalam waktu lama pada suhu -80 ° C. Teknik PCR konvensional dilakukan untuk mendeteksi gen LMP1 dengan mengamplifikasi DNA gen EBV LMP1 yang menghasilkan amplikon DNA berukuran 142 bp. Analisis sensitivitas metode PCR dan hasil identifikasi gen LMP-1 dilakukan menggunakan metode deskriptif dengan membandingkan hasil deteksi yang diperoleh pada penelitian ini dengan hasil penelitian terdahulu yang terkait. Hasil penelitian menunjukkan bahwa gen LMP-1 EBV sebagai biomarka diagnosis KNF dapat dideteksi menggunakan teknik PCR konvensional yang menghasilkan amplikon DNA berukuran 142 bp. Sensitivitas gen LMP1 adalah 77,27%, yang menunjukkan sensitivitas tinggi. Sensitivitas PCR konvensional dalam mendeteksi gen EBMP LMP-1 lebih tinggi bila dibandingkan dengan gen EBMP LMP-1 30 bp dan LMP-2A EBV.
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