Characterization of Bifidobacteria from infant feces with different mode of birth at Purwokerto, Indonesia

Abstract

Bifidobacteria belongs to the so-called beneficial intestinal flora. Before attempting to raise their intestinal levels to improve the health status of the host, it is importance to know about physiological variations in the Bifidobacterial colonization of the human intestine. Birth process influenced the diversity of Bifidobacteria in infant feces. This research was intended to isolate and characterize Bifidobacterium spp. as well to evaluate their presence in the feces of infants who were born by mode of normal, caesarean and premature. The research was conducted by survey method and data were analyzed with descriptive analysis. Bifidobacterium character was observed include colony and cell morphology. The biochemical test included catalase, oxidase, indole, Voges-Proskauer, different pH growth, and resistance to lysozyme. Bifidobacterium metabolites obtained tested its bacterial activity to Salmonella typhimurium and Escherichia coli. The result of this research showed that 35 isolates are suspected Bifidobacterium group and after API 20 A test showed 17 isolates are rally genera of Bifidobacterium spp.and all isolates come from infant feces with caesar and premature delivery. These isolates inhibited the growth of S. typhimurium and E. coli with different inhibitory capabilities. This finding is very important for science and medical point of view and could be developed with further research.

Bifidobacteria and Escherichia coli Microbiota of Healthy Indonesian Infants in Andalas Village: Profile of Infant Diet Given Exclusive Breastfed and Formula-fed

“…The milky white colonies have a flat edge/smooth (entire), some are uneven such as wool, round, shiny, and convex elevation with varied sizes. These isolates have morphological characters referring to Bifidobacterial genus, that is, colony with milky white color or slightly creamy, rounded form with the diameter of 0.1-0.5 mm as well as Gram-positive (Figure 1) [9], [10], [11], [12]. The morphology of E. coli colonies of the isolated bacteria exhibited pink color, small rod shaped, and Gram-negative bacilli (Figure 2) [13].…”

Potential Screening of Bacteriocinogenic-Lactic Acid Bacteria from Mangrove Sediment of Logending Beach for Fisheries Product Preservation

“…Bacterial incubation was carried out for 48 hours at 37 o C. Isolates that expressed a clear zone around the growing colony were assumed as Lactic Acid Bacteria. The bacterial characterization was performed following Hendrati et al (2017). The selected isolates were stocked on MRSA slant medium.…”

“…One loop of LAB isolate was inoculated in MRS Broth (MRSB) medium and incubated for 18 hours at 37 o C. The bacterial culture was centrifuged for 10 minutes at 10,000 rpm to obtain a bacterial cell-free supernatant (CFS). Its inhibition activity was evaluated using Kirby's Bauer method by following Hendrati et al (2017). The pathogenic bacteria were inoculated on Nutrient Broth (NB) medium (1% v/v), then was incubated for 8 hours at 37 o C. The pathogenic bacteria were spread on NA medium.…”

“…A total of 200 µL partially-purified bacteriocin was mixed with 20 µL of proteolytic enzyme solution (one gram of proteolytic 'papain' enzymes was dissolved into 1 mL of 0.1 M PBS with pH 5.3), then was incubated at 37 o C for 2 hours. Its inhibition activity was evaluated using Kirby's Bauer method by following Hendrati et al (2017). The degraded bacteriocin did not show a clear zone around the paper disc (Nithya et al 2012).…”

“…The inhibition activity of crude bacteriocin was evaluated using Kirby's Bauer method following by following Hendrati et al (2017). The diameter of the inhibition zone expressed by CFS and partially-purified bacteriocin were then compared to evaluate the success of bacteriocin extraction.…”