Potential Screening of Bacteriocinogenic-Lactic Acid Bacteria from Mangrove Sediment of Logending Beach for Fisheries Product Preservation

Abstract

The meat and fisheries products have high nutritional content which is highly ideal for bacterial growth. Lactic Acid Bacteria (LAB) have several potential advantages as a bio-preservative agent in the food industry because they produce antimicrobial substances against pathogenic bacteria e.g. bacteriocin. Our previous study has succeeded in isolating and characterizing LAB from the mangrove sediments of Logending Beach, Kebumen. This present study aimed to determine the activity of bacteriocinogenic-LAB against food-borne pathogens and their potential for fisheries product preservation. The study consisted of five serial stages, as follows: screening of LAB isolates, cell-free supernatant production and its inhibition activity, extraction of partially purified bacteriocin, bacteriocin confirmation against proteolytic enzymes, and in-vitro test of partially-purified bacteriocin against Listeria monocytogenes, Shigella flexneri, and Salmonella typhi. A total of 25 out of 99 isolates were able to grow on MRSA+1% CaCO3 medium. Initial screening showed that the cell-free supernatant of 14 LAB isolates was able to inhibit the growth of S. thypi, S. flexneri, and L. monocytogenes. There was an increased inhibitory activity of partially purified bacteriocin when compared with the cell-free supernatant which was statistically different (p<0.01). It indicated that the purification was successfully performed. Bacteriocin expressed a lower inhibition against S. typhi than L. monocytogenes and S. flexneri. The ANOVA test showed that each indicator pathogenic-bacterium expresses a very significant sensitivity to the partially purified bacteriocin.

Isolation and Identification of Lactobacillus plantarum C010 and Growth Kinetics of its Batch Fermentation

“…Chen et al showed inhibitory activity of Lactobacillus plantarum CKXP13 and CWXP24 against the indicator bacteria even after neutralization pH and degradation of hydrogen peroxide, and these antibacterial substances were confirmed as bacteriocins (Chen et al 2021). In addition, the LABs isolated and screened from the mangrove sediments of Logending Beach, Kebumen by Dyah Fitri Kusharyat, also exhibited antibacterial activity after crude extraction with ammonium sulfate (Kusharyati et al 2021).…”

Lactic Acid Bacteria from Mangrove Sediment Produce Bacteriocins Active Against Gram-Positive and Negative Bacteria

“…Previous research has succeeded in discovering several isolates of "lactic acid bacteria from mangrove sediments at Logending Beach in Jawa Tengah (Kusharyati et al, 2021). This present study aimed to determine the characteristics of LAB isolates from mangrove sediment, bacteriocin activity as an antimicrobial against Staphylococcus aureus and Escherichia coli, and the physicochemical characteristics of bacteriocins.…”

“…The liquid culture was centrifuged at 5,480 x g for 15 minutes at 4 °C. The resulting cell-free supernatant (CFS) was separated using the salting-out method by adding ammonium sulfate (Kusharyati et al, 2021). A solution of ammonium sulfate (60%) was slowly added until homogeneous.…”

“…Papain was used to analyze the sensitivity of crude bacteriocin against proteolytic enzymes (Kusharyati et al, 2021). A total of 200 μl of crude bacteriocin was added, along with 20 μl of papain enzyme (the pH of the mixture was adjusted to 7).…”

Bacteriocinogenic Lactic Acid Bacteria Isolated from Mangrove Sediment in Indonesia: Growth Optimization, Bacteriocin Production, and its Application in Food Preservation

“…However, there still needs to be more research exploring lactic acid bacteria (LAB) from mangrove ecosystems. Kusharyati et al (2021) isolated potential LAB from mangrove sediments in Logending Beach, Central Java (Indonesia). Potential screening is carried out through producing bacteriocins and their antimicrobial activity.…”