This research aimed to identify LMP-1 EBV gene as a biomarker in NPC diagnosis. Cross-sectional study with consecutive sampling technique was applied. Samples were the whole blood of NPC WHO-3 patients collected from those untreated since 2014 in the Department of Otorhinolaryngologica, Prof. dr. Margono Soekarjo Hospital, Purwokerto. A total of 22 research subjects for NPC WHO-3 patients were completed with informed consent. The samples were subjected to DNA isolation by using Purelink® DNA/RNA viral (Invitrogen) kit protocols to obtain 100µL DNA solution, which were then stored for a long period of time at -80°C. Conventional PCR technique was performed to detect LMP1 gene to amplify the DNA of LMP1 gene resulting in a DNA amplicon of 142 bp. Analysis of the PCR method sensitivity and the results of LMP-1 gene identification were carried out descriptively by comparing the detection results obtained in this study with the results of previous ones related. The sensitivity of LMP1 gene is 77.27%, indicating a high sensitvity.The results show that LMP-1 gene as a biomarker in the diagnosis of NPC can be detected using conventional PCR technique. Conventional PCR sensitivity in the detection of LMP1gene shows higher sensitivity in compare to LMP-1 EBV gene deletion of 30 bp and LMP2A. Keywords: Epstein-Barr Virus, LMP-1 gene, Diagnosis, Nasopharyngeal Carcinoma AbstrakPenelitian ini bertujuan untuk mengidentifikasi gen LMP-1 EBV sebagai biomarka diagnosis KNF. Desain penelitian ini adalah studi cross-sectional dengan teknik consecutive sampling. Sampel adalah darah total pasien KNF WHO-3 yang dikumpulkan dari pasien yang belum menjalani terapi dari tahun 2014 pada Departemen Telinga Hidung Tenggorok -Kepala Leher, Rumah Sakit Prof. dr. Margono Soekarjo, Purwokerto. Total subyek penelitian adalah 22 orang untuk NPC WHO-3 pasien dengan informed consent. Sampel diisolasi dengan protokol kit Purelink® DNA / RNA (Invitrogen) untuk mendapatkan larutan DNA 100μL dan disimpan dalam waktu lama pada suhu -80 ° C. Teknik PCR konvensional dilakukan untuk mendeteksi gen LMP1 dengan mengamplifikasi DNA gen EBV LMP1 yang menghasilkan amplikon DNA berukuran 142 bp. Analisis sensitivitas metode PCR dan hasil identifikasi gen LMP-1 dilakukan menggunakan metode deskriptif dengan membandingkan hasil deteksi yang diperoleh pada penelitian ini dengan hasil penelitian terdahulu yang terkait. Hasil penelitian menunjukkan bahwa gen LMP-1 EBV sebagai biomarka diagnosis KNF dapat dideteksi menggunakan teknik PCR konvensional yang menghasilkan amplikon DNA berukuran 142 bp. Sensitivitas gen LMP1 adalah 77,27%, yang menunjukkan sensitivitas tinggi. Sensitivitas PCR konvensional dalam mendeteksi gen EBMP LMP-1 lebih tinggi bila dibandingkan dengan gen EBMP LMP-1 30 bp dan LMP-2A EBV.
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