The gingival index (GI) and the Orogranulocytic Migratory Rate (OMR) were determined for 86 subjects. The statistical analyses revealed a highly significant correlation between the two parameters. An analysis of regression demonstrated that the correlation of GI and OMR changed with low to high values of GI, probably because GI is not a true parametric index.
– The rate of cell proliferation in homogeneous leukoplakias was compared with that of normal mucosa. Biopsies from six patients were labeled in vitro with tritiated thymidine and the labeling indices relative to (1) the total cell population, (2) the progenitor compartment, and (3) 100 μm surface length, were determined on autoradiographs. In leukoplakias, cell renewal and mitotic cycles were faster than in the epithelium of controls.
Glucocorticoid administration is known to have an inhibitive effect on collagen activity. It can be assumed that this effect also takes place in the periodontal membrane (PDM). The purpose of the present investigation was to investigate whether this reduction in PDM collagen activity is reflected in the autoradiographic grain count score. Ten young adult male squirrel monkeys were injected with JH-proline and sacrificed 48 hours after the injection. Five of the animals were injected with Ultracortenole 72 and 24 hours prior to sacrifice. Grain count analysis was carried out on autoradiographs from sections of premolars, and skin biopsies were analyzed with respect to NaCl soluble 3H-activity. In the skin the activity found in the soluble fraction was lower in the Ultracortenol-treated than in the control animals (319 f 56 cpmlmg compared to 629 f 70 cpmlmg). Autoradiographic grain counts were carried out over the periodontal membrane, the average number of grains was lower in the Ultracortenolinjected than in the control animals. This was true for all areas of the periodontal membrane examined. The significance of the autoradiographic grain count score from the PDM following 3H-proline injection is discussed. It is concluded that this parameter is ieasonably, valid for the assessment of the PDM collagen activity.In the last decades considerable research activity has been focused on collagen metabolism, and in particular the collagen activity in skin has been thoroughly investigated. Tracer studies on experimental animals have revealed that the rate of renewal is dependent on the age of the animals; the formation of new collagen being more rapid in young than in old animals (Nimni, G u i a 6 Bavetfa 1965). The collagen activity is also influenced by other factors, e. g. glucocorticoids depress the rate of formation (Kowalewski 1966, Laitinen 1967.Attempts have also been made to analyze collagen synthesis in the periodontal membrane (PDM). Such analyses present technical obstacles since the necessary analyses for biochemical assay of the PDM, particularly in smaller animals, are difficult to apply.The only possibility for assessment of the PDM collagen activity has hitherto been autoradiography following injection of 3H-proline. Stallard (1963), C a r n e i r o (1965), and C a r n e i r o 6 Moraes (1965) demonstrated collagen synthesis in the PDM autoradiographically after injection of labeled proline or glycine. S k o u g a a r d , Levy 6Simpson (1970) found by the same method that the activity in PDM was extremely high, approximately eight times higher than in skin and twice as high as in gingiva. Furthermore it was dem-
Abstract— The purpose of the present work was to investigate possible age changes in the rate of collagen formation in the periodontal membrane and to compare the collagen activity in the periodontal membrane with that found in the skin. Twenty‐three marmosets (one baby, 14 young adults, and 8 old animals) were injected with 3H‐proline and sacrificed 2, 7, and 21 days after injection. Autoradiographs were made from the periodontal tissues and from skin biopsies. The latter were furthermore analyzed with respect to total 3H‐activity and specific hydroxyproline activity. In the periodontal membrane the grain counts were highest in the baby and higher in the young adult than in the old marmosets. The same pattern was observed in the skin. Here the grain counts were 8 times lower than in the periodontal membrane. Furthermore the autoradiographs revealed that the collagen activity in the gingival connective tissue was only half of that found in the periodontal membrane. It was concluded that the higher activity in the periodontal membrane was caused by a higher collagen concentration compared to gingival connective tissue and to skin.
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