Four-Factor Model Analysis of Thymidine Incorporation into Mouse DNA and the Mechanism of Radiation Effects

Stewart, Peter A.; Quastler, Henry; Skougaard, M. R.; Wimber, Doris R.; Wolfsberg, Marilyn F.; Perrotta, Carmie A.; Ferbel, Barbara; Carlough, M.

doi:10.2307/3571871

Cited by 38 publications

(12 citation statements)

References 5 publications

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“…That the treatment schedule reported by Ganzer et al (1974) may yield good clinical results is not disputed and has been confirmed by other authors (Helpap et al, 1977;Wannenmacher et al, 1974). However, as pointed out by these other authors, the positive clinical results probably depend upon factors other than synchronization.…”

Section: In Vitro and In Vivo Studies With Animalssupporting

confidence: 57%

“…However, the efficacy of vincristine as a synchronizing agent has been disputed (Jellinghaus et al, 1975). In addition, Wannenmacher et al (1974) were unable to confirm the results obtained by Nitze et al (1974) with FU.…”

contrasting

confidence: 45%

“…The rate of TdR infusion was 3 ,ug/mouse/min. This rate was calculated, from the work of Stewart et al (1965) and Lee et al (1976) to be sufficient to provide the mice with enough TdR for normal requirements in the absence of de novo synthesis. A drop of ascitic fluid was withdrawn from each mouse 8 times during a 28-h period.…”

Section: Biochemical Action Of 5-fluorouracilmentioning

confidence: 99%

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In vivo cell synchrony in the L1210 mouse leukaemia studied with 5-fluorouracil or 5-fluorouracil followed by cold thymidine infusion

Camplejohn¹,

Schultze²,

Maurer³

1977

Br J Cancer

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Summary.-[3H]-TdR and [3H]-UdR labelling indices and mitotic indices werefollowed in tumour-bearing mice after application of either 5-fluorouracil (FU) alone or of FU followed by cold TdR infusion. With FU alone, accumulation of cells at the beginning of S was found, but there was no indication of a synchronous passage of the accumulated cells further round the cycle. When FU injection was followed by cold TdR infusion, a synchronous passage of the accumulated cells through the cycle was observed. However, there was a large variation in the response of individual mice to this treatment.

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Section: In Vitro and In Vivo Studies With Animalssupporting

confidence: 57%

contrasting

confidence: 45%

Section: Biochemical Action Of 5-fluorouracilmentioning

confidence: 99%

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In vivo cell synchrony in the L1210 mouse leukaemia studied with 5-fluorouracil or 5-fluorouracil followed by cold thymidine infusion

Camplejohn¹,

Schultze²,

Maurer³

1977

Br J Cancer

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“…Male Wistar rats (250 g) were injected with drugs in 0.9% saline, and 27 h later 0 25 mCi/kg [6-3H] (Cleaver, 1967;Stewart, Quastler, Skougaard, Wimber, Wolfsberg, Perrotta, Ferbel & Carlough, 1965 …”

Section: Methods [6-3h]thymidine Incorporationmentioning

confidence: 99%

The Effect of Carbachol and Isoprenaline on Cell Division in the Exocrine Pancreas of the Rat

Carling¹,

Templeton²

1982

Exp Physiol

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SUMMARYThe effect of muscarinic cholinergic receptor stimulation on pancreatic acinar cell division has been investigated. Muscarinic receptor stimulation, in addition to causing a 50% depletion of amylase in the pancreas after 4 h, resulted in a 400% increase in the incorporation of [6-3H]thymidine 27 h after drug administration. This increase in incorporation was dosedependent. Autoradiography showed this increase to be due to an increased acinar cell labelling. Isoprenaline also increased the incorporation of [6-3H]thymidine into the pancreas but did not reduce enzyme levels. However, with isoprenaline there was no increase in acinar cell labelling.The possibility of a relationship between secretion and hyperplasia of acinar cells in the rat pancreas is discussed.

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“…6). Lymphocytes pulse-labeled for 6 hr with 0.05 pC/ml may not have been significantly perturbed due to the slow rate at which such a dilute concentration of precursor would be incorporated (22,26). Such a low rate of 3H-TdR incorporation would allow for more repair of intranuclear 3H damage.…”

Section: H-tdr Radiation Effects In Human Lymphocytesmentioning

confidence: 99%

The kinetics of the formation of a G2 block from tritiated thymidine in phytohemagglutinin‐stimulated human lymphocytes

et al. 1980

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Flow cytometry (FCM) was used to monitor the radiation effects promoted by incorporated tritiated thymidine (3H‐TdR) on phytohemagglutinin (PHA)‐stimulated human peripheral blood lymphocytes stained with propidium iodide (PI). Lymphocyte microcultures were continuously labeled or pulse‐labeled for various periods of time with different 3H‐TdR concentrations. Two types of DNA histogram analyses wer performed on unperturbed and 3H‐TdR perturbed lymphocytes. The data analyses consisted of statistical analyses between averaged groups of histograms (nonparametric analysis) and cell cycle analyses (parametric analysis) to determine the percentages of cells in G0 + G1, S and G2 + M. The results showed that (a) 3H‐TdR when added to proliferating lymphocytes under certain conditions (both short‐term continous and pulse‐labeling) caused a highly significant increase in the proportion of tetraploid (4C) cells by FCM, (b) the increase in the proportion of 4C cells represented a block in G2 and (c) the relative increase in the percentage of 4C cells was proportional to 3H‐TdR incorporation which was proportional to labeling time and concentration. Therefore, it was concluded that short labeling times be used to minimize adverse radiation effects when 3H‐TdR is used to assay substances affecting lymphocyte proliferation or in the estimation of cell cycle time.

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Four-Factor Model Analysis of Thymidine Incorporation into Mouse DNA and the Mechanism of Radiation Effects

Cited by 38 publications

References 5 publications

In vivo cell synchrony in the L1210 mouse leukaemia studied with 5-fluorouracil or 5-fluorouracil followed by cold thymidine infusion

In vivo cell synchrony in the L1210 mouse leukaemia studied with 5-fluorouracil or 5-fluorouracil followed by cold thymidine infusion

The Effect of Carbachol and Isoprenaline on Cell Division in the Exocrine Pancreas of the Rat

The kinetics of the formation of a G2 block from tritiated thymidine in phytohemagglutinin‐stimulated human lymphocytes

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