UJI KEMAMPUAN <i>Lactobacillus casei</i> SEBAGAI AGENSIA PROBIOTIK

Abstract

Growth Ability of Jatropha Curcas L. Explants on the In Vitro Media Containing IBA and BAProbiotic product is one of the applications of biotechnology that utilize lactic acid bacteria, especially lactobacilli. Some important requirements for microbes that can be used as probiotic include resistance to low pH, ability to grow on bile salts and colonize, and having antimicrobial activity. Each species of the genus Lactobacillus has different characteristics. These characteristics are strongly influenced by the environment in which the bacteria live. This study was carried out in order to characterize Lactobacillus casei which was isolated from dadih. The result of the experiment showed that the isolated L. casei was able to grow on the bile salt at the concentration of 15%, resistant to acid media until pH 2, had antimicrobial activity (significantly inhibited the growth of Escherichia coli, Staphylococcus aureus, and Enterococcus faecalis). The local L. casei isolate has a potential application for use as probiotic microbe.Keywords: Lactobacillus casei, probiotic, lactic acid bacteria, characterization,dadih ABSTRAKProduk probiotik merupakan salah satu aplikasi bioteknologi yang memanfaatkan bakteri asam laktat terutama jenis Lactobacillus. Beberapa syarat utama mikroba yang dapat difungsikan sebagai mikroba probiotik antara lain tahan terhadap pH rendah, mampu tumbuh pada garam empedu, mampu berkoloni, memiliki aktivitas antimikroba. Masing-masing spesies dari Genus Lactobacillus memiliki karakteristik yang berbeda-beda. Karakteristik ini sangat dipengaruhi oleh lingkungan dimana bakteri tersebut hidup. Pada penelitian ini telah dilakukan karakterisasi Lactobacillus casei yang merupakan hasil isolasi dari susu kerbau fermentasi. Dari hasil percobaan menunjukkan bahwa L.casei hasil isolasi mampu hidup sampai dengan konsentrasi garam empedu 15%, tahan terhadap media asam sampai dengan pH 2, memiliki aktivitas antimikroba (positif menghambat Escherichia coli, Staphylococcus aureus, dan Enterococcus faecalis). L. casei yang merupakan isolat lokal memiliki karakteristik yang berpotensi untuk digunakan sebagai mikroba probiotik.Kata kunci: Lactobacillus casei, probiotik, bakteri asam laktat, karakterisasi, dadih

Karakteristik Minuman Probiotik Jambu Biji (Psidium guajava) pada Berbagai Variasi Penambahan Sukrosa dan Susu Skim

“…Lactobacillus casei merupakan bakteri asam laktat yang dapat digunakan sebagai starter dalam pembuatan minuman probiotik dari sari buah (Ranadheera et al, 2010). Spesies ini efektif sebagai bakteri probiotik karena kemampuannya untuk bertahan hidup terhadap asam lambung dan garam empedu dalam sistem pencernaan manusia (Sunaryanto et al, 2014). Selama fermentasi mikroba akan berinteraksi langsung dengan media yang mengandung nutrisi.…”

Feeding combination of Lactobacillus casei and extracts of dahlia tuber or garlic on intestinal bacteria, nutrients digestibility and performance of broiler chickens

“…It can be postulated that the improved bacterial balance is in concomitant with the increased short-chain fatty acids, a fermentation product due to the biological activity of beneficial effects of LAB. In addition, Lactobacillus casei produced anti-microbial substances that can suppressed the growth of pathogenic bacteria (Sunaryanto et al, 2014). Nutrient Digestibility Synbiotics composed of L. casei and dahlia tuber extract, as well as L. casei and garlic extract improved digestion process, based on protein and crude fiber digestibility.…”

“…One of the potential bacteria that can function as probiotics is Lactobacillus casei. Probiotic from Lactobacillus casei could produce anti-microbial substances and suppressed the growth of pathogenic bacteria (Sunaryanto et al, 2014). The existence of this probiotic bacteria would be successfully improved when fed together with prebiotic inulin as a "source of nutrient," called as a synbiotic or just a status of combination.…”

Screening of Probiotic Bacteria Candidates in The Mangrove Tourism Area in Klawalu Sorong City West Papua

“…Genus Lactobacillus memiliki warna koloni putih susu, bentuk koloni bulat dengan tepian seperti wol. Sel berbentuk batang dan merupakan bakteri gram positif (Sunaryanto et. al., 2014).…”