Screening of Probiotic Bacteria Candidates in The Mangrove Tourism Area in Klawalu Sorong City West Papua

Sukmawati, Sukmawati; Badaruddin, M Iksan

doi:10.24036/0201932105397-0-00

Cited by 3 publications

(3 citation statements)

References 11 publications

(11 reference statements)

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“…Previously, a study was conducted on screening probiotic bacteria candidates in the Klawalu subdistrict, Sorong City, West Papua Province. The results of the study indicated that 9 isolates had potential as lactic acid bacteria (Sukmawati and Badaruddin 2019). However, exploration of nitrogen-fixing bacteria at the genetic level in mangrove ecosystems has not been conducted.…”

Section: Introductionmentioning

confidence: 96%

Short Communication: Bacterial diversity of mangrove ecosystem in Klawalu Sorong, West Papua, Indonesia

et al. 2022

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Abstract. Sukmawati S, Rosalina F, Sipriyadi, Dewi NK, Yunita M, Sarhan ART, Rahayu Y, Kusumawati E. 2022. Short Communication: Bacterial diversity of mangrove ecosystem in Klawalu Sorong, West Papua, Indonesia. Biodiversitas 23: 1427-1432. The mangrove ecosystem is a producer of detritus and a source of nutrients and organic matter. Some of the ecological functions of mangrove forests are coastline protector, preventing seawater intrusion, as a habitat for various living creatures, a microclimate regulator, a nursery ground, spawning ground, as well as a feeding ground for various aquatic biota. The mangrove forest ecosystem cannot be separated from the role of microbes in helping the process of soil biochemical cycles. In the biochemical cycle, microbes are able to maintain the availability of macronutrients in the soil. The objective of this study was to identify the diversity of bacteria in the mangrove ecosystem in Klawalu, Sorong City. The research method descriptively described the diversity of bacterial species found in the mangrove ecosystem in Klawalu, Sorong City, West Papua Province. The results indicated that the DNA fragments of the four isolates obtained from this study were around 1300 bp. Meanwhile, the bacterial species obtained were isolated SA3, identified as Bacillus safensis strain C251, isolate SA8 identified as Bacillus amyloliquefaciens strain NO10, isolate SL8 was identified as Clostridium sp. JC336, and isolate SL1 was identified as Bacillus australimaris strain IIHR GAPB01.

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Section: Introductionmentioning

confidence: 96%

Short Communication: Bacterial diversity of mangrove ecosystem in Klawalu Sorong, West Papua, Indonesia

et al. 2022

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“…Amylase enzyme production is influenced by several factors and in general, amylase enzymes are optimally generated at a certain point in the logarithmic phase of the microbial growth curve, but there are certain microbes where the logarithmic phase is longer so that the amylase enzyme is not produced because the point has not been reached so long incubation is influential in this matter (Gunam, et al, 2012;Sukmawati & Badaruddin, 2019 ). The temperature can affect because there are some thermophilic bacteria that are more optimal bacteria in the production of amylase enzymes in higher temperature conditions (Supriyanti & Heryanto, 2013).…”

Section: Figure 1 Standard Curve Of Maltose Solutionmentioning

confidence: 99%

Measurement of alpha-Amilase Enzymes in Bacillus subtilis Bacteria

Sukmawati

Yunita

Rosalina

2021

indonesian.j.of.biology.education

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The bacterium Bacillus subtilis is able to produce amylase enzymes which are able to hydrolyze various types of starch sources into simple compounds such as maltose, glucose. Amylase converts carbohydrates which are polysaccharides into maltose. Amylase enzyme is a group of enzymes that are needed in the industrial field. The purpose of this study was to determine the level of the amylase enzyme produced by the bacterium Bacillus subtilis. The method used in this research is a quantitative method using an amylase enzyme measurement experiment. The results of this study are from two types of enzyme sources, namely Bacillus subtilis + Starch + NB and Bacillus subtilis + NB. Both samples showed enzyme activity. The conclusion of this study is that Bacillus subtilis + Starch + NB produces 63.186 units of the alpha-amylase enzyme used to produce 1 ppm / 1 micromol maltose per minute. Whereas the Bacillus subtilis + NB sample was 19.474 units of the alpha-amylase enzyme used to produce 1 ppm / 1 micromol maltose per minute.

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“…According to previous research results, no one has explored probiotic bacteria from mangrove areas. Thus, researchers conducted a bacterial screening as a probiotic candidate in the Klawalu mangrove tourism area of Sorong City, West Papua and found that of the 16 mud and water samples, 11 samples were detected as probiotic bacterial candidates (Sukmawati and Badaruddin, 2019). Furthermore, the study continued by identifying potential probiotic bacteria molecularly and the molecular identification results revealed three potential bacterial isolates as probiotic candidates, namely Bacillus safensis strain C251 SA3, Bacillus amyloliquefaciens strain NO10 SA8 and Bacillus australimaris strain IIHR GAPB01 SL1 (Sukmawati et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Pathogenicity analysis and application of probiotic bacteria in Catfish (Clarias sp.) cultivation in vivo

Sukmawati, S.,

Fahrizal, A.,

Yunita, M.

2024

MJM

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Aims:The aim of this study was to analyze the physiological properties, pathogenicity and application of probiotic bacteria to catfish (Clarias sp.) cultivation in vivo. Methodology and results: Probiotic bacteria's physiological characteristics were described using descriptive approaches, whereas experimental methods analyzed pathogenicity tests and the application of the probiotic bacteria in catfish cultivation. The results of bacterial characterization showed that Bacillus safensis strain C251 SA3, Bacillus amyloliquefaciens strain NO10 SA8 and Bacillus australimaris strain IIHR GAPB01 SL1 had bacili cell shape and showed a positive catalase test for all test bacteria except Bacillus safensis strain C251 SA3. The three probiotic bacteria also had negative oxidative-fermentative results, which were able to reduce nitrate and survive in environmental conditions with pH 3-9. Pathogenicity analysis revealed that the three bacteria had the potential to be non-pathogenic or less pathogenic. Bacillus safensis strain C251 SA3 treatment on catfish resulted in the best survival rate of 64% and a 17.5 cm growth in catfish body length by week 13. When Bacillus amyloliquefaciens strain NO10 SA8 was applied to catfish, the survival rate was 32% and the catfish body length increased by 22 cm by week 13. The control treatment revealed that all catfish samples died or had a 0% survival rate, while the application of Bacillus australimaris strain IIHR GAPB01 SL1 revealed a catfish survival rate of 0%. Conclusion, significance and impact of study: Bacillus australimaris strain IIHR GAPB01 SL1 is a potential probiotic bacterium to be applied in catfish food.

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Screening of Probiotic Bacteria Candidates in The Mangrove Tourism Area in Klawalu Sorong City West Papua

Cited by 3 publications

References 11 publications

Short Communication: Bacterial diversity of mangrove ecosystem in Klawalu Sorong, West Papua, Indonesia

Short Communication: Bacterial diversity of mangrove ecosystem in Klawalu Sorong, West Papua, Indonesia

Measurement of alpha-Amilase Enzymes in Bacillus subtilis Bacteria

Pathogenicity analysis and application of probiotic bacteria in Catfish (Clarias sp.) cultivation in vivo

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