Organogênese do caquizeiro a partir de segmentos radiculares

Carvalho, Dirceu Tornavoi de; Biasi, Luiz Antônio

doi:10.1590/s0103-84782004000500012

Cited by 2 publications

(4 citation statements)

References 14 publications

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“…Carvalho & Biasi (2004) observed similar results with BAP and KIN in in vitro multiplication of "caqui". On the other hand, Fráguas et al (2004) reported that the best growth of woody plants in vitro was obtained using BAP instead of KIN.…”

Section: Resultssupporting

confidence: 73%

“…However, ZEA has shown itself to be very efficient at eliciting budding in some species, such as kaki, (Diospyros kaki) (Carvalho & Biasi, 2004), blueberry (Vaccinium ashei) (Silva et al, 2006), and Vaccinium vitis (Debnath & Mcrae, 2002). Although TDZ is considered one of the most potent cytokinins, principally among woody plants, the reduced growth rate observed in buds of A. glabra at concentrations of 2mg L -1 were very similar to the results obtained by Akasaka et al (2000) -who noted a negative effect of this cytokinin on in vitro development of Arachis hypogaea.…”

Section: Resultsmentioning

confidence: 99%

“…Carvalho & Biasi (2004) demonstrated that the amount of zeatin (ZEA) and indoleacetic acid (AIA) used to induce budding in explants of "caqui" (Diospyros kaki) can be reduced to 1µM and 0.01µM, respectively, when these explants are cultivated in MS medium (Murashige & Skoog, 1962) containing only half the normal concentration of NO 3 . It has also been suggested that the use of growth regulators (especially cytokinins) affects the rates of absorption and translocation of essential minerals in plants under in vitro conditions as they directly influence the formation and differentiation of the vascular system and the regulation of enzymes involved in the assimilatory metabolism of minerals, principally nitrogen (Aloni, 2001;Donato et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Effects of cytokinins on in vitro mineral accumulation and bud development in Annona glabra L.

et al. 2010

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Annona glabra is a tropical species that has significant agronomic potential in terms of furnishing fruits for in natura consumption and for the production of phyto-pharmaceuticals. In vitro cultivation has been considered the most promising form of propagation for this species, although large scale utilization of this technique is currently limited by high rates of leaf abscission, reduced rates of explant multiplication and slow bud growth. The present work evaluated the effects of different cytokinins on mineral accumulation in shoots of A. glabra cultivated in vitro, and their effects on growth and survival of these plants. Buds of A. glabra were cultivated in Wood Plant Medium (WPM) in the presence of 6-benzilaminopurine (BAP), thidiazuron (TDZ), kinetin (KIN), and zeatin (ZEA). KIN and BAP use resulted in the greatest growth, largest accumulation of dry mass and leaf area development, as well as the greatest survival rate during in vitro cultivation of this species. All cytokinins tested stimulated large accumulations of nitrogen and boron in shoots, but diminished levels of calcium as compared to controls.Index terms: Growth regulators, Annonaceae, mineral nutrition. RESUMOAnnona glabra é uma espécie frutífera tropical que apresenta elevado potencial agronômico pelo fornecimento de frutos para o consumo in natura e pela produção de fitofármacos. O cultivo in vitro tem sido preconizado como a forma mais adequada de propagação para essa espécie, embora sua utilização em larga escala ainda seja limitada pela elevada taxa de abscisão foliar, reduzida taxa de multiplicação dos explantes e crescimento lento das brotações. Objetivou-se, neste trabalho, avaliar o efeito de citocininas sobre o acúmulo de minerais nas brotações de A. glabra cultivadas in vitro e seus reflexos sobre o crescimento e sobrevivência das plantas nesse tipo de ambiente. Brotações de A. glabra foram cultivadas em meio Wood Plant Medium (WPM), na presença de 6-benzilaminopurina (BAP), thidiazuron (TDZ), cinetina (KIN) e zeatina (ZEA). A utilização de KIN e BAP induziu maior crescimento, maior acúmulo de massa seca, maior desenvolvimento da área foliar e maior taxa de sobrevivência das plantas durante o cultivo in vitro dessa espécie. Todas as fontes de citocininas testadas estimularam maiores acúmulos de nitrogênio e boro nas brotações e menores de cálcio.Termos para indexação: Reguladores de crescimento, Annonaceae, nutrição mineral.

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Section: Resultssupporting

confidence: 73%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of cytokinins on in vitro mineral accumulation and bud development in Annona glabra L.

et al. 2010

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“…(Peres et al 2001), Diospyros kaki (Carvalho and Biasi 2004), Helianthus annuus 9 H. tuberosus (Fambrini et al 2003), Melia azedarach (Vila et al 2005), Elentherococcus koreanum (Park et al 2005), Arabidopsis (Atta et al 2009), Azadirachta indica (Arora et al 2010), Cleome rosea (Simões et al 2009), Solanum melongena (Xing et al 2010), Brassica oleraceae var Italica (Yang et al 2010), among others.…”

Section: Introductionmentioning

confidence: 99%

Organogenesis from root explants of commercial populations of Passiflora edulis Sims and a wild passionfruit species, P. cincinnata Masters

Silva¹,

Oliveira²,

Loriato³

et al. 2011

Plant Cell Tiss Organ Cult

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Root explants of a wild passionfruit species (Passiflora cincinnata) and three P. edulis commercial populations ('FB 100', 'FB 200', and 'FB 300') were incubated on Murashige and Skoog (MS) medium supplemented with 4.44 lM 6-benzyladenine (BA) to induce shoot organogenesis. Shoots elongated in liquid medium with 2.89 lM gibberellic acid (GA 3 ) under agitation were rooted in coconut fiber and acclimatized followed by transfer to a greenhouse into pots containing mixture of coconut fiber and Plantmax Ò (1:1). Explant samples were collected during organogenesis and submitted to light and scanning electron microscopy (SEM). Root explants of P. cincinnata responded earlier than those of P. edulis. However, on the third assessment, at 90 days, the genotype 'FB 200' showed shoot number significantly higher than 'FB 100' and 'FB 300', not differing from P. cincinnata.Organogenesis in P. cincinnata and P. edulis occurred via direct pathway, which was confirmed by anatomical studies and SEM. Flow cytometric analysis revealed no variation in DNA content of regenerated plantlets among all genotypes. Nuclear DNA (2C) values (pg) in regenerants of P. cincinnata (2.99 pg) and P. edulis (3.26-3.28 pg) were consistent with DNA amounts of seed-derived control plants.

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Organogênese do caquizeiro a partir de segmentos radiculares

Cited by 2 publications

References 14 publications

Effects of cytokinins on in vitro mineral accumulation and bud development in Annona glabra L.

Effects of cytokinins on in vitro mineral accumulation and bud development in Annona glabra L.

Organogenesis from root explants of commercial populations of Passiflora edulis Sims and a wild passionfruit species, P. cincinnata Masters

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