Effects of cytokinins on in vitro mineral accumulation and bud development in Annona glabra L.

Abstract

Annona glabra is a tropical species that has significant agronomic potential in terms of furnishing fruits for in natura consumption and for the production of phyto-pharmaceuticals. In vitro cultivation has been considered the most promising form of propagation for this species, although large scale utilization of this technique is currently limited by high rates of leaf abscission, reduced rates of explant multiplication and slow bud growth. The present work evaluated the effects of different cytokinins on mineral accumulation in shoots of A. glabra cultivated in vitro, and their effects on growth and survival of these plants. Buds of A. glabra were cultivated in Wood Plant Medium (WPM) in the presence of 6-benzilaminopurine (BAP), thidiazuron (TDZ), kinetin (KIN), and zeatin (ZEA). KIN and BAP use resulted in the greatest growth, largest accumulation of dry mass and leaf area development, as well as the greatest survival rate during in vitro cultivation of this species. All cytokinins tested stimulated large accumulations of nitrogen and boron in shoots, but diminished levels of calcium as compared to controls.Index terms: Growth regulators, Annonaceae, mineral nutrition. RESUMOAnnona glabra é uma espécie frutífera tropical que apresenta elevado potencial agronômico pelo fornecimento de frutos para o consumo in natura e pela produção de fitofármacos. O cultivo in vitro tem sido preconizado como a forma mais adequada de propagação para essa espécie, embora sua utilização em larga escala ainda seja limitada pela elevada taxa de abscisão foliar, reduzida taxa de multiplicação dos explantes e crescimento lento das brotações. Objetivou-se, neste trabalho, avaliar o efeito de citocininas sobre o acúmulo de minerais nas brotações de A. glabra cultivadas in vitro e seus reflexos sobre o crescimento e sobrevivência das plantas nesse tipo de ambiente. Brotações de A. glabra foram cultivadas em meio Wood Plant Medium (WPM), na presença de 6-benzilaminopurina (BAP), thidiazuron (TDZ), cinetina (KIN) e zeatina (ZEA). A utilização de KIN e BAP induziu maior crescimento, maior acúmulo de massa seca, maior desenvolvimento da área foliar e maior taxa de sobrevivência das plantas durante o cultivo in vitro dessa espécie. Todas as fontes de citocininas testadas estimularam maiores acúmulos de nitrogênio e boro nas brotações e menores de cálcio.Termos para indexação: Reguladores de crescimento, Annonaceae, nutrição mineral.

Assessing the response of indigenous loquat cultivar Mardan to phytohormones for in vitro shoot proliferation and rooting

“…Barberaki and Kintzios (2002) reported that the addition of macronutrients in plant tissues is significantly affected by the addition of PGRs. Kinetin and BAP enhance leaf area and maximum dry weight accumulation (de Oliveira et al, 2010). Increases in cytokinin levels lead to increases in the numbers of leaves and shoots, and in the rooting percentage by triggering cell division and elongation (Schmulling et al, 2003).…”

Efficient regeneration and Agrobacterium-mediated transformation protocol for recalcitrant indica rice (Oryza sativa L.).

“…The ALA-D enzyme catalyzed the porphyrin ring formation which was used in chlorophyll and cytochrome synthesis. Explants cultivated with BAP and KIN also showed a higher accumulation of nitrogen resulting in thicker, darker green leaves as well as higher dry weight comparing to TDZ and ZEA (Oliveira et al, 2010). To conclude, KIN could be a potential cytokinin in multiplying in-vitro rice shoots with higher chlorophyll content which was associated with better plant vigour and quality.…”

In <i>Vitro</i> Clonal Propagation from Adult Material of a Savannah Species of Socio-Economic Importance: <i>Annona senegalensis</i> Pers.

“…According to [22], depending on the origin of the explants, two methods are conventionally used: disinfection with mercuric chloride, generally used for potentially infected material, and disinfection with calcium hypochlorite. Several researchers [23] [24] [25] [26]) used the first method to disinfect explants of Jatropha curcas originating from greenhouses, nurseries or fields or nodal explants of Tectona grandis, a woody tree [27].…”