Organogenesis from root explants of commercial populations of Passiflora edulis Sims and a wild passionfruit species, P. cincinnata Masters

Abstract

Root explants of a wild passionfruit species (Passiflora cincinnata) and three P. edulis commercial populations ('FB 100', 'FB 200', and 'FB 300') were incubated on Murashige and Skoog (MS) medium supplemented with 4.44 lM 6-benzyladenine (BA) to induce shoot organogenesis. Shoots elongated in liquid medium with 2.89 lM gibberellic acid (GA 3 ) under agitation were rooted in coconut fiber and acclimatized followed by transfer to a greenhouse into pots containing mixture of coconut fiber and Plantmax Ò (1:1). Explant samples were collected during organogenesis and submitted to light and scanning electron microscopy (SEM). Root explants of P. cincinnata responded earlier than those of P. edulis. However, on the third assessment, at 90 days, the genotype 'FB 200' showed shoot number significantly higher than 'FB 100' and 'FB 300', not differing from P. cincinnata.Organogenesis in P. cincinnata and P. edulis occurred via direct pathway, which was confirmed by anatomical studies and SEM. Flow cytometric analysis revealed no variation in DNA content of regenerated plantlets among all genotypes. Nuclear DNA (2C) values (pg) in regenerants of P. cincinnata (2.99 pg) and P. edulis (3.26-3.28 pg) were consistent with DNA amounts of seed-derived control plants.

Effect of plant growth regulators and antioxidants on in vitro plant regeneration and callus induction from leaf explants of purple passion fruit (Passiflora edulis Sims)

“…Several studies also reported that the plant regeneration of passion fruit could be obtained via organogenesis from a wide range of species and types of explants such as leaf, hypocotyl, root and cotyledon (Braglia et al 2010;Fernando et al 2007;Lombardi et al 2007;Nhut et al 2007;Pinto et al 2010;Silva et al 2011). Two morphogenic pathways are known to be related to in vitro plant differentiation and regeneration process, de novo organogenesis and somatic embryogenesis (Rochas et al 2015).…”

Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit

“…is a key tool for the production of clones with enhanced resistance and other desirable traits. Numerous tissue culture studies in Passiflora have been conducted and regeneration by organogenesis has been successfully reported from a wide range of species (Fernando et al 2007;Lombardi et al 2007;Pinto et al 2010;Silva et al 2011;Anand et al 2012). However, this is not the case for P. gibertii, where there have been limited studies on in vitro cultivation (Faria et al 2006;Faria et al 2007;Carvalho et al 2013).…”

“…such as P. cincinnata Mast. (Lombardi et al 2007;Silva et al 2011), P. suberosa L. (Garcia et al 2011), P. edulis Sims and P. alata Curtis (Pacheco et al 2012). Coconut water contains substances that are essential for the growth and for regeneration of many species such as P. edulis x P. edulis var flavicarpa (Hall et al 2000), P. edulis (Fernando et al 2007) and P. alata Curtis (Pacheco et al 2012).…”

“…It is used as a growth supplement in plant tissue culture and its chemical composition is rich in sugars, vitamins, minerals, amino acids and phytohormones such as cytokinins that stimulate the division of mature plant cells (Yong et al 2009). Histological, morphological and ultrastructural analyses can be performed to monitor the regeneration and to differentiate organogenesis (Fernando et al 2007;Silva et al 2011;Rocha et al 2012) from somatic embryogenesis (Pinto et al 2011). By characterizing the regeneration path, the best growth conditions can be established allowing the development of efficient protocols for the induction and production of plants.…”

Scanning Electron Microscopy Studies and In Vitro Regeneration of Passiflora edulis Sims var. edulis for Conservation

“…Tissue culture studies of Passiflora started as early as 1966 and since then many reports on tissue culture based methods applied to the genus have been published [6]. Most of the in vitro culture techniques for Passiflora were developed using Passiflora edulis Sims f. flavicarpa Deg.…”