Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit

Carvalho, Milene Alves de Figueiredo; Paiva, Renato; Stein, Vanessa Cristina; Herrera, Raírys Cravo; Porto, Jorge Marcelo Padovani; Vargas, Daiane Peixoto; Alves, Eduardo

doi:10.1590/s1516-8913201402555

Cited by 6 publications

(2 citation statements)

References 31 publications

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“…Also, we tested the putative influence of dark (D) incubation versus standard incubation (S). Dark incubation has been reported as positive for organogenesis induction in some species (Zhao et al 2013;Carvalho et al 2014). Four cultivars (AVA8,cv.27,cv.28,and cv.34) that showed high B values and differing R in the previous assay were compared in this assay.…”

Section: Resultsmentioning

confidence: 99%

Improvement of regeneration in pepper: a recalcitrant species

Gammoudi

Pedro

Ferchichi

et al. 2017

In Vitro Cell.Dev.Biol.-Plant

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Organogenesis is influenced by factors like genotype, type of explant, culture medium components, and incubation conditions. The influence of ethylene, which can be produced in the culture process, can also be a liming factor in recalcitrant species like pepper. In this work, bud induction was achieved from cotyledons and hypocotyls -from eight pepper cultivars -on Murashige and Skoog (MS) medium supplemented with 22.2 µM 6-benzyladenine (6BA) and 5.7 µM indole-3-acetic acid (IAA), in media with or without silver nitrate (SN) (58.9 µM), a suppressor of ethylene action. In the SN-supplemented medium, the frequencies of explants with buds and with callus formation were lower in both kinds of explant, but higher numbers of developed shoots were isolated from explants cultured on SN. Bud elongation was better in medium with gibberellic acid (GA3) (2.9 µM) than in medium free of growth regulators or supplemented with 1aminocyclopropane-1-carboxylic acid (ACC) at 34.5 µM. However, isolation of shoots was difficult and few plants were recovered. The effect of adding SN following bud induction (at 7 d) and that of dark incubation (the first 7 d of culture) was also assessed in order to improve the previous results. When SN was added after bud induction, similar percentages of bud induction were found for cotyledons (average frequency 89.4% without SN and 94.4% with SN) whereas they doubled in hypocotyls (50 % without SN and 87.7% with SN). In addition, in both kinds of explant, the number of developed plants able to be transferred to soil (developed and rooted) was greatly increased by SN. Dark incubation does not seem to improve organogenesis in pepper, and hypocotyl explants clearly represent a better explant choice -with respect to cotyledonary explants -for the pepper cultivars assayed. REGENERATION IN PEPPER3

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Section: Resultsmentioning

confidence: 99%

Improvement of regeneration in pepper: a recalcitrant species

Gammoudi

Pedro

Ferchichi

et al. 2017

In Vitro Cell.Dev.Biol.-Plant

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“…The surface ultrastructure of many plants derived tissue cultured has been reported widely, such as in Passiflora spp. (Carvalho et al 2014), Zea mays (Mendez et al 2009), and Canna indica L. (Wafa et al 2016). In tea, but not derived from tissue culture, there were many reports about surface ultrastructure analysis such as white tea leaves (Ekayanti et al 2017), trichomes of green tea (Liang et al 1993), and polysaccharides of green tea (Yi et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture

Eskundari

Taryono

Indradewa

et al. 2021

J.Tropical Biodiversity Biotechnology

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Tissue culture is an alternative choice of plant propagation either through somatic embryogenesis or in vitro organogenesis techniques. TRI2025 tea clone has been cultured successfully, however, the scientific information related to morphology, histology, and protein profile at an early event of culturing time has not been reported yet. This study aimed to determine the differences between those pathways, in the context of morphology, histology, and protein profile. The explants were the embryo axis of TRI2025 tea clone cultured on two different induction mediums; somatic embryogenesis and in vitro organogenesis induction medium. The results showed that most of the explants cultured on A medium developed to be a globular-like structure at 11-day after culture (DAC), while all explants cultured on B medium showed the initiation stage of in vitro organogenesis. Histological analysis showed meristem reconstruction at shoot apical meristem (SAM) and root apical meristem (RAM) at 11-DAC at explants cultured on B medium, while explants cultured on A medium showed callusing at 21-DAC. Protein profile analysis using SDS-PAGE showed protein bands of 54 and 81 KDa that only appeared at explants cultured on A medium start from 14-DAC, and those two protein bands thought to be a differentiator at the early stages of the two tissue culture techniques. Thus, these parameters can be used as early detection for plant tissue culture, especially in tea.

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Morphology and ontogeny of directly differentiating shoot buds and somatic embryos in Santalum album L.

Akhtar

Shahzad

2018

J. For. Res.

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Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit

Abstract: This work studied a new protocol for organogenic calli induction and characterization of the morphology and

Cited by 6 publications

References 31 publications

Improvement of regeneration in pepper: a recalcitrant species

Improvement of regeneration in pepper: a recalcitrant species

Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture

Morphology and ontogeny of directly differentiating shoot buds and somatic embryos in Santalum album L.

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