Tea (Camellia sinensis L.) is classified as cross-pollinated crop and vegetative multiplication becomes commercially the main method of propagation with some limitations such as high heterogeneity and poor in survival rate and also in rooting. A proven tissue culture method, somatic embryogenesis, is the only challenging way to meet the needs of tea seedlings in large quantities. The study was conducted with TRI2025 tea clone selected from Polyclonal garden of PT. Pagilaran (Batang, Central Java). The explants were cultured on MS media supplemented with four concentrations of 2,4-D (0, 1, 2, and 5 mg L-1) in two incubation conditions; dark and light. The results showed the only concentration of 2,4-D that can induce somatic embryo was 2 mg L-1 2,4-D in light condition and its percentage was about 5%. Other concentrations of 2,4-D that given for treatments both in two conditions will not induce somatic embryo. This study needs more improvements for getting powerful and efficient of method to get somatic embryo-derived plant and also for futher successful genetic engineering of tea biotechnology.

Ecoenzymes are natural ingredients formed from the fermentation of fruit and or vegetable residues that have many benefits. One of the benefits of ecoenzymes is that they can be used as eco-handsanitizers; which can be used as a candidate for handsanitizer for those who are allergic to alcohol-based handsanitizer. This study aims to determine the pH, antibacterial, antiviral, and antifungal content of the candidate eco-handsanitizer solution through alkaloid, flavonoid, and saponin test, also fungal-inhibition test. The candidate eco-handsanitizer solution was made from an ecoenzyme solution derived from fruit or vegetable residue and added with aloe vera gel and vitamin C or E. The results showed that eco-handsanitizer candidate solution derived from ecoenzyme solution with a dilution of 1:500 or 1:4 and added with aloe vera and vitamin E had a pH that tends to be acidic after 5 weeks of manufacture. The candidate eco-handsanitizer solution contained alkaloid compounds up to 2nd week for 1:500 dilution or up to 5th week for 1:4. Fungal test also showed positive results of one of the treatments in inhibiting fungal growth until the 11th day. These results were expected to be used as guidelines for further research, such as quantitative and qualitative tests for more sensitive alkaloids, for example using GC-MS.ÂÂ

Tea is well known as favourite healthy drink for almost all people over the world. Tea propagation using conventional and modern ways are now developing rapidly. However, information regarding the protein profile of tissue culture of tea plant has not been revealed yet. This study aimed to determine the difference of protein profile of tea's tissue culture using SDS-PAGE. This study was conducted using embryonic axes of TRI2025 tea clone cultured on MS media supplemented with 2,4-D for inducing somatic embryogenesis and globular-like structure (GLS) regeneration, and MS media supplemented with BAP for inducing shoot via organogenesis. The results revealed that proteins in the size of 37.69; 54.89; 60.77; 71.35; 87.34; and 92.99 KDa might be involved at somatic embryogenesis, and about 38.69 KDa, 69.27 KDa, and 55.76 KDa respectively for GLS, initiation of shoot, and initiation of GLS derived leaf. Predicted key protein for leaf initiation both directly or through GLS was about 31-33 KDa, while for callusing were about 27.56 and 52.73 KDa, and constitutive protein was about 22.75 KDa. This study provides the first report of protein profile of tea's tissue culture. The information obtained can be beneficial as a marker for explant for somatic embryogenesis, GLS, or organogenesis pathway and as a scientific information for further biotechnology development.

Limbah rumah tangga berupa minyak jelantah dan sisa sayur-buah tetap ada setiap hari di hampir setiap rumah tangga di masyarakat mitra. Tujuan pengabdian ini adalah memanfaatkan limbah minyak jelantah dan diolah menjadi pengharum ruangan yang ramah lingkungan khususnya oleh masyarakat mitra. Metode yang digunakan dalam pengabdian ini adalah metode ceramah dan pendampingan praktek langsung. Materi yang disampaikan adalah pengolahan minyak jelantah dan ekoenzim menjadi pengharum ruangan. Observasi awal keadaan mitra terlihat bahwa sebagian besar wanita di Dukuh Bener RT01/04 Kelurahan Tepisari Kecamatan Polokarto merupakan ibu rumah tangga dengan persentase penggunaan minyak goreng yang cukup tinggi tiap bulan. Selain itu, limbah rumah tangga lainnya berupa sisa sayur dan buah jumlahnya banyak per hari dan masyarakat mitra telah mendapatkan pelatihan pembuatan ekoenzim. Kegiatan Pengabdian dilakukan dengan metode ceramah serta praktek langsung. Materi yang disampaikan dapat terserap dengan baik oleh mitra sehingga mitra sudah dapat praktek langsung dalam membuat pengharum ruangan. Hasil posttest menunjukkan bahwa kegiatan ini berhasil mencapai tujuannya yaitu dengan adanya nilai 100 %. Dengan demikian, kegiatan ini diharapkan dapat menjadi alternatif pengolahan limbah rumah tangga sekaligus dapat dijadikan sumber pendapatan tambahan masyarakat.

Residents of the village of Gabahan Rt. 05 Rw. Most of Jombor's 12 sub-districts work as farm laborers and hand laborers and desperately need additional income to meet the needs of their families. They have never received counseling or training on organic composting by universities or other institutions. From the results of the interview, several residents wanted counseling and training on the manufacture of organic compound fertilizer as an alternative to increase financial income. They want counseling with this theme because most of them do not understand how to make organic compost. The purpose of the service is 1. To provide counseling on how to make organic compost. 2. To provide training on how to make and pack organic compost. The training was carried out for 2 times. The method used is lectures and questions and answers, as well as practical guidance on how to make and pack organic compost. The target of this counseling and training is to increase the understanding of how to make 20% organic compost among the residents of Gabahan RT. 05 RW. 12 Jombor. From the results of the training in making organic compost for PKK women in the hamlet of Gabahan RT 05 RW 12 Jombor, it can be concluded that there was an increase in understanding of the process of making organic compost from an average score of 37.5 to 88.45 or an increase in understanding of 122, 4%. The results of the compost can be used by women as plant fertilizer, but can also be sold to increase income.