Cultura de embriões in vitro para o melhoramento de pessegueiros precoces

Barbosa, Wilson; Dall’Orto, Fernando Antônio Campo; Ojima, Mário

doi:10.1590/s0006-87051985000100044

Cited by 8 publications

(5 citation statements)

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“…Valores seguidos de mesma letra na coluna não diferem entre si pelo teste de Duncan (α = 5%) 1 Escala de Notas: 0 -sem nenhuma raiz; 1-menos de 5 raízes; 2 -de 5 a 10 raízes; 3 -de 11 a 20 raízes e 4 -mais de 21 raízes. 2 Escala de Notas: 1 -embrião morto; 2 -branco ou verde, mas sem parte aérea; 3 -fraco ou com ponta seca; 4 -crescimento pouco vigoroso e 5 -crescimento normal, vigoroso.…”

Section: Experimentounclassified

“…Desde então a técnica de cultura de embriões vem se expandindo e contribuindo significativamente com estudos básicos de fisiologia do desenvolvimento de embrião (Carvalho & Araújo, 2007). A má germinação das sementes de pessegueiros precoces tem explicação no processo de maturação do fruto, em que o rápido endurecimento do caroço restringe o acúmulo de matéria orgânica na semente e causa um desenvolvimento anormal do embrião, tornando-o pouco vigoroso e incapaz de germinar naturalmente (Barbosa et al, 1985).…”

Section: Introductionunclassified

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Resgate De Embriões Em Pessegueiro: Tempo De Incubação

Raseira

Einhardt²

2010

RSA

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RESUMOO sucesso no desenvolvimento de plântulas, a partir de embriões imaturos tem sido obtido desde 1936. Entretanto, quanto menor é o embrião, mais complexo e difícil é alcançar a porcentagem desejável de sobrevivência. O objetivo do presente trabalho foi avaliar o período de tempo que as sementes de clones muito precoces deveriam ficar em meio de cultura e à temperatura de 22 ±1 °C, antes de serem submetidas à embriocultura. Também foi avaliado o efeito de se conservar as frutas em câmara fria, por uma semana, antes de ser feito o resgate de embriões. Os resultados indicam que o tempo para incubação dos mesmos, à temperatura de 22 ±1 °C pode ser de até seis semanas, mas o melhor é entre três e quatro semanas. O fator limitante é a contaminação das sementes por patógenos. Também é possível obter sucesso com a técnica de resgate de embriões de frutas conservadas por uma semana em câmara fria, desde que o tempo de incubação não seja superior a três ou quatro semanas.Palavras-chave: embriocultura; embriões imaturos; Prunus persica. ABSTRACTSuccess with immature embryos has been achieved since 1936. However, as smaller is the embryo more complex and difficult is to have a desirable survival percentage. The objective of this work was to check the period of time that seeds should be kept in the culture media at 22 ±1 °C, before being submitted to the embryo culture procedure. The effect of keeping the fruits for a week in cold room, previously to the ovule rescue, was also evaluated. Results showed that the time for ovule incubation at 22 ±1 °C, can be up to nine weeks but better three to five weeks. The limiting factor is related to seed contamination by pathogens. It is also possible to have success with ovule rescue of embryos of fruits kept for one week in cold room, as long as the time, the ovule incubation period, is not superior to three or four weeks.

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Section: Experimentounclassified

Section: Introductionunclassified

Resgate De Embriões Em Pessegueiro: Tempo De Incubação

Raseira

Einhardt²

2010

RSA

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“…The rapid hardening of the stone reduces the accumulation of organic matter in the seed, causing abnormality in embryo development, preventing germination from occurring under natural conditions. This phenomenon hinders the formation of seedlings in sufficient volume, hindering the continuity of genetic breeding programs for peach trees (BARBOSA et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

Culture media and bap concentrations in the embryo culture of ‘BRS Kampai’ peach

et al. 2020

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Early-ripening peach cultivars present difficulties in the process of natural germination of seeds. In order to solve this problem, the culture of embryos in vitro can be considered an alternative, in which seeds find conditions to complete germination and development in a satisfactory way. Different protocols and culture media have been tested to meet the nutritional needs of the embryo, but they still have problems and need to be optimized. The objective of this work was to test culture media and BAP concentrations in order to increase the germination percentage of embryos and viable ‘BRS Kampai’ peach seedlings, avoiding possible anomalies in the process. The experiment was carried out at the Laboratory of Tissue Culture – “Universidade Tecnológica Federal do Paraná”, Campus of Pato Branco. The experimental design used was randomized blocks, in 2x5 factorial, with four replicates, with plot being represented by 15 embryos. The culture media tested were MS and WPM with five BAP concentrations (0, 1, 2, 3 and 4 mg L-1). In the in vitro period, seed germination was evaluated through the attribution of scores, and embryo development, measuring the length of stem and main root. During the acclimatization period, seedlings were evaluated for survival, stem length, viable seedlings and rosette formation. Considering the conditions under which the experiment was conducted, it was concluded that MS culture medium with the addition of 1 mg L-1 of BAP allowed higher germination percentage and viable ‘BRS Kampai’ peach seedlings.

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“…Seeds from mother plants whose fruits have a short fruit development period may present germination problems because the embryo is immature at the time of harvest. However, embryo culture is a technique that makes possible the germination and development of immature embryos, being essential in a breeding program [2,3,4]. Without this technique, early ripening peach cultivars could not be used as parents, since the percentage of germination would be very low [5].…”

Section: Introductionmentioning

confidence: 99%

Germination and Development of 'Precocinho' Peach Embryos: Asepsis and Use of PPMTM in Culture Medium

Nascimento¹,

Dini²,

Carpenedo³

et al. 2018

JEAI

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Embryo culture is a technique used to rescue immature embryos which would not germinate under natural conditions. However, one of the biggest problems of this process is the contamination by microorganisms. The objective of this work was to test asepsis methods and the use of PPM TM (Plant Preservative Mixture TM ) in the culture medium for the germination and development of 'Precocinho' peach embryos. After extraction from the fruits, seeds were submitted to treatments with thimerosal, sodium hypochlorite (NaClO), flame sterilization and moxifloxacin hydrochloride, in culture medium with and without addition of PPM TM , totaling nine treatments. The experimental design was completely randomized, with 20 replicates per treatment. Three evaluations were performed: in vitro establishment (at 55 days of cultivation), embryo development (at 65 days of cultivation) and acclimatization (20 days after transplanting in the greenhouse). Six treatments did Original Research ArticleTM is shown as the most efficient treatment, among the tested ones, for the germination and development of 'Precocinho' peach embryos.

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Cultura de embriões in vitro para o melhoramento de pessegueiros precoces

Cited by 8 publications

References 8 publications

Resgate De Embriões Em Pessegueiro: Tempo De Incubação

Resgate De Embriões Em Pessegueiro: Tempo De Incubação

Culture media and bap concentrations in the embryo culture of ‘BRS Kampai’ peach

Germination and Development of 'Precocinho' Peach Embryos: Asepsis and Use of PPMTM in Culture Medium

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