Germination and Development of 'Precocinho' Peach Embryos: Asepsis and Use of PPMTM in Culture Medium

Abstract

Embryo culture is a technique used to rescue immature embryos which would not germinate under natural conditions. However, one of the biggest problems of this process is the contamination by microorganisms. The objective of this work was to test asepsis methods and the use of PPM TM (Plant Preservative Mixture TM ) in the culture medium for the germination and development of 'Precocinho' peach embryos. After extraction from the fruits, seeds were submitted to treatments with thimerosal, sodium hypochlorite (NaClO), flame sterilization and moxifloxacin hydrochloride, in culture medium with and without addition of PPM TM , totaling nine treatments. The experimental design was completely randomized, with 20 replicates per treatment. Three evaluations were performed: in vitro establishment (at 55 days of cultivation), embryo development (at 65 days of cultivation) and acclimatization (20 days after transplanting in the greenhouse). Six treatments did Original Research ArticleTM is shown as the most efficient treatment, among the tested ones, for the germination and development of 'Precocinho' peach embryos.

Conservação in vitro da aroeira (myracrodruon urundeuva fr. Allemão) e estabelecimento in vitro da baraúna (schinopsis brasiliensis engl.)

“…O uso de hipoclorito de sódio também foi a assepsia mais eficiente para a germinação e desenvolvimento de embriões de pêssego (NASCIMENTO et al, 2018). É válido ressaltar, que a dosagem do hipoclorito de sódio é fator importante na desinfestação.…”