Characterization, cytotoxic and antioxidant potential of silver nanoparticles biosynthesised using endophytic fungus (Penicillium citrinum CGJ-C1)

Danagoudar, Ananda; Pratap, G.; Shantaram, Manjula; Ghosh, Krishna; Kanade, Santosh R.; Joshi, Chandrashekhar G.

doi:10.1016/j.mtcomm.2020.101385

Cited by 27 publications

(13 citation statements)

References 27 publications

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“…Other signals were also reported, probably due to elements from proteins and enzymes present in the extract. These findings of the EDX profile are in line with the results of Hu et al (2019) and Danagoudar et al (2020); they described well‐dispersed spherical nanoparticles from the extracellular filtrate of endophytic fungi.…”

Section: Discussionsupporting

confidence: 91%

“…The FTIR spectra exhibited the existence of several functional groups that are connected with AgNPs. This reveals that the silver reduction and capping of the obtained AgNPs are carried out by the proteins and metabolites secreted by fungi into the CF (Danagoudar et al, 2020; Hu et al, 2019; Hulikere & Joshi, 2019). The carbonyl groups of amino acid likely acted as a capping agent of AgNPs (Sowmyya & Vijaya Lakshmi, 2018).…”

Section: Discussionmentioning

confidence: 97%

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Biosynthesis of silver nanoparticles using endophytic Fusarium oxysporum strain NFW16 and their in vitro antibacterial potential

Ilahi

Haleem

Iqbal

et al. 2021

Microscopy Res & Technique

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Nanotechnology has provided a platform for altering, modifying, and developing metal properties to nanoparticles with promising applications. This study aimed to produce functionalized and biocompatible silver nanoparticles (AgNPs) using cellular extracts of endophytic Fusarium oxysporum-NFW16 isolated from Taxus fauna and evaluate its antibacterial potential. Under optimized reaction conditions, welldispersed and extremely stable AgNPs were synthesized in 1 hr. AgNPs were characterized through UV-visible spectrophotometry (at 423 nm), and scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The obtained AgNPs were spherical, monodispersed, and size was $30-36.1 nm. Strong peaks of XRD (311), ( 220), (200), and (111) matched to silver plane's diffraction facets. FTIR spectra at 1,650, 2,950, and 1,400 cm À1 confirmed the capping of AgNPs with phenolic compounds and compounds having primary amines. The AgNPs showed 100 μg/ml of minimum inhibitory concentration against methicillin-resistant Staphylococcus aureus (MRSA). In addition, AgNPs showed a synergistic effect with both vancomycin and ciprofloxacin against MRSA (25%), Pseudomonas aeruginosa (50%), and pus isolated Escherichia coli (50%).Moreover, AgNPs impregnated cotton and bandage showed in vitro antibacterial potential against American Type Culture Collection and skin-associated clinical pathogenic bacteria. Findings showed that endophytic fungi are the potential source for AgNPs synthesis that are effective against multidrug-resistant bacteria and the development of antimicrobial textile finishes.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 97%

Biosynthesis of silver nanoparticles using endophytic Fusarium oxysporum strain NFW16 and their in vitro antibacterial potential

Ilahi

Haleem

Iqbal

et al. 2021

Microscopy Res & Technique

View full text Add to dashboard Cite

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“…FTIR spectra indicate the presence or attachment of various biomolecules secreted by fungi on the surface of AgNPs. At optimum conditions, the incorporation of silver salt into CFF solution results in immediate binding of silver ions with the protein and other molecules present in CFF solution with functional groups such as –OH and C=C being captured, resulting in conformational changes in proteins that represent its hydrophobic residues to the aqueous phase, resulting in infiltration of reducing agents from CFF solution and thus providing capping to silver ions with the formation of stable nanoparticles (Danagoudar et al, 2020; Osorio‐Echavarría et al, 2021). Assuming the CFF of the marine fungi Penicillium oxalicum and Fusarium hainanense used in this study, it is conceivable that alkyne, sulphurous and phenolic compounds, proteins, alcohol and other water‐soluble biomolecules contributed as reducing and stabilizing agents to the silver nanoparticles (Guilger‐Casagrande & Lima, 2019).…”

Section: Discussionmentioning

confidence: 99%

Biogenic synthesis of silver nanoparticles mediated by the consortium comprising the marine fungal filtrates of Penicillium oxalicum and Fusarium hainanense along with their antimicrobial, antioxidant, larvicidal and anticancer potency

Thakor

Mistry

Patel³

et al. 2022

Journal of Applied Microbiology

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Aim: To biosynthesize silver nanoparticles (AgNPs) using fungal isolates [DS-2 (Penicillium oxalicum) and DW-8 (Fusarium hainanense)] as well as their mixed cellfree filtrate (CFF) acting as a consortium (DSW-28) and their bio-potentials. Methods and Results:The fungi (DS-2 and DW-8) were harvested and CFF was prepared. CFF of each fungus and their mixture were reacted with silver nitrate solution under dark conditions for the synthesis of AgNPs. The UV-Visible spectra determined the surface plasmon resonance at 438, 441 and 437 nm for the AgNPs synthesized by DS-2, DW-8 and DSW-28, respectively. The band gap energy was found between 2.21 and 2.24 eV which depicted their ability to act as a semiconductor. The TEM imaging revealed the spherical shape and small size of AgNPs. The XRD pattern exhibited the crystalline structure corresponding to their peaks. The FTIR spectra indicate the presence of different functional groups present on the surface of AgNPs. The broad-spectrum antimicrobial activity was exhibited by AgNPs. The AgNPs also act as an effective antioxidant by depicting their radical scavenging activity against DPPH. Moreover, the AgNPs also inhibited the growth of fourth instar larvae of Aedes aegypti and Culex quinquefasciatus more efficiently in a dosedependent method. The biosynthesized AgNPs from DSW-28 showed a significant anticancer activity against MCF-7 cells. Conclusion:The silver nanoparticles synthesized by the CFF of two different fungi act synergistically in a consortium leading towards the production of silver nanoparticles with smaller size and higher bioactivity. Significance and Impact of the Study:The impressive bioactivity of the silver nanoparticles synthesized by the mixture of CFF of various fungi acting as a consortium recommends their prospective use in agriculture as well as in biomedical as an antimicrobial, antioxidant, larvicidal and anticancer agents in future.

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“…2 Nanoparticles are mainly synthesized via three approaches: physical method, chemical method, and biological method. 3 An alternative method is needed due to the use of toxic and very precious chemicals in addition to high temperature in the synthesis phase of NPs by chemical and physical methods. 4 The green synthesis method of NPs worths special regard owing to its low cost, eco-friendly, high yield and nontoxicity.…”

Section: Introductionmentioning

confidence: 99%

“…4 The green synthesis method of NPs worths special regard owing to its low cost, eco-friendly, high yield and nontoxicity. 3,4 Among the metal NPs, AgNPs one of the most notable nanomaterial specially due to their biological features. In numerous studies by different researchers demonstrated the antibacterial, antifungal, anticancer, antimutagenic action, antiviral, antioxidant, and the wound healing properties of AgNPs.…”

Section: Introductionmentioning

confidence: 99%

Yenilebilir mantar Macrolepiota procera kullanılarak yeşil sentez yöntemiyle sentezlenen gümüş nanopartiküllerinin in-vitro biyolojik aktivitelerinin araştırılması

Gonca

2022

Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi Ve Folklorik Tıp Dergisi

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Aim: Mushrooms known that to be used in traditional treatment among the people as they can generate a large diversity of secondary metabolites. In the present study, it was aimed to synthesized silver nanoparticles (AgNPs) mediated Macrolepiota procera, which is known to have diverse biological activities such as anticancer, antioxidant, antimicrobial in previous studies, and to investigated various in-vitro biological activities of these AgNPs. Materials and Methods: Synthesized Mp-AgNPs were characterized using transmission electron microscopy (TEM). Various biological activities including antimicrobial, biofilm inhibition and cell viability inhibition, DNA cleavage, DPPH activity of synthesized AgNPs were investigated in-vitro. Results: The highest DPPH scavenging activity of Mp-AgNPs was found as 92.72%, at 200 mg/L concentration. Mp-AgNPs caused single strain break in the E. coli pBR322 plasmid DNA. Mp-AgNPs showed moderate antimicrobial activity against tested microorganisms. Furthermore, the biofilm inhibition activity of Mp-AgNPs toward P. aeruginosa and S. aureus was 65.80% and 72.60% at 200 mg/L, respectively. Inhibition activity of E. coli cell viability of Mp-AgNPs was found as 99.99% at 500 mg/L. Conclusion: From the findings obtained in the study , the use of M. procera in the AgNPs synthesis it is important private regard due to its low cost, eco-friendly, high yield and non-toxicity human health. In addition, newly synthesized AgNPs can be used effectively for different applications after further studies.

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Characterization, cytotoxic and antioxidant potential of silver nanoparticles biosynthesised using endophytic fungus (Penicillium citrinum CGJ-C1)

Cited by 27 publications

References 27 publications

Biosynthesis of silver nanoparticles using endophytic Fusarium oxysporum strain NFW16 and their in vitro antibacterial potential

Biosynthesis of silver nanoparticles using endophytic Fusarium oxysporum strain NFW16 and their in vitro antibacterial potential

Biogenic synthesis of silver nanoparticles mediated by the consortium comprising the marine fungal filtrates of Penicillium oxalicum and Fusarium hainanense along with their antimicrobial, antioxidant, larvicidal and anticancer potency

Yenilebilir mantar Macrolepiota procera kullanılarak yeşil sentez yöntemiyle sentezlenen gümüş nanopartiküllerinin in-vitro biyolojik aktivitelerinin araştırılması

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