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Introduction: Dental trauma has emerged as a significant aspect of dental public health. Traumatized teeth require early and appropriate treatment, which frequently relies on the competence of the dentists, doctors, and nurses who provide the primary care. Aim: To assess the knowledge and attitude of paramedical students about the emergency management of avulsed tooth. Materials and Methods: A cross-sectional questionnaire survey consisting of 17 questions was designed and circulated among 365 paramedical students (nursing, pharmacy and physiotherapy) of Sankalchand Patel University, Visnagar, Gujarat, India, to assess their knowledge and attitude about the emergency management of avulsed tooth. Descriptive statistics were applied by Statistical Package for Social Sciences (SPSS) software version 20.0. Results: Total 260 (71.23%, mean age of 23 years) students responded, with 71 students from Physiotherapy, 106 from Nursing and 83 from Pharmacy. A total of. 178 (68.46%) students comprehend the meaning of avulsion. While 193 (74.23%) students had knowledge that tooth re-implantation is possible. Of all the respondents, 192 students (73.84%) were familiar about the benefit of mouth guards for prevention of sports injury and 125 students (48.07%) opined that it is very important to be acquainted with emergency management of dental trauma. Conclusion: Though the paramedical students had good understanding about tooth avulsion, they were unaware about the emergency management of the situation. This warrants the need of incorporating training programmes dedicated to the subject in their curriculum.

Aim: To biosynthesize silver nanoparticles (AgNPs) using fungal isolates [DS-2 (Penicillium oxalicum) and DW-8 (Fusarium hainanense)] as well as their mixed cellfree filtrate (CFF) acting as a consortium (DSW-28) and their bio-potentials. Methods and Results:The fungi (DS-2 and DW-8) were harvested and CFF was prepared. CFF of each fungus and their mixture were reacted with silver nitrate solution under dark conditions for the synthesis of AgNPs. The UV-Visible spectra determined the surface plasmon resonance at 438, 441 and 437 nm for the AgNPs synthesized by DS-2, DW-8 and DSW-28, respectively. The band gap energy was found between 2.21 and 2.24 eV which depicted their ability to act as a semiconductor. The TEM imaging revealed the spherical shape and small size of AgNPs. The XRD pattern exhibited the crystalline structure corresponding to their peaks. The FTIR spectra indicate the presence of different functional groups present on the surface of AgNPs. The broad-spectrum antimicrobial activity was exhibited by AgNPs. The AgNPs also act as an effective antioxidant by depicting their radical scavenging activity against DPPH. Moreover, the AgNPs also inhibited the growth of fourth instar larvae of Aedes aegypti and Culex quinquefasciatus more efficiently in a dosedependent method. The biosynthesized AgNPs from DSW-28 showed a significant anticancer activity against MCF-7 cells. Conclusion:The silver nanoparticles synthesized by the CFF of two different fungi act synergistically in a consortium leading towards the production of silver nanoparticles with smaller size and higher bioactivity. Significance and Impact of the Study:The impressive bioactivity of the silver nanoparticles synthesized by the mixture of CFF of various fungi acting as a consortium recommends their prospective use in agriculture as well as in biomedical as an antimicrobial, antioxidant, larvicidal and anticancer agents in future.

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