Biosynthesis of silver nanoparticles using endophytic Fusarium oxysporum strain NFW16 and their in vitro antibacterial potential

Abstract

Nanotechnology has provided a platform for altering, modifying, and developing metal properties to nanoparticles with promising applications. This study aimed to produce functionalized and biocompatible silver nanoparticles (AgNPs) using cellular extracts of endophytic Fusarium oxysporum-NFW16 isolated from Taxus fauna and evaluate its antibacterial potential. Under optimized reaction conditions, welldispersed and extremely stable AgNPs were synthesized in 1 hr. AgNPs were characterized through UV-visible spectrophotometry (at 423 nm), and scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The obtained AgNPs were spherical, monodispersed, and size was $30-36.1 nm. Strong peaks of XRD (311), ( 220), (200), and (111) matched to silver plane's diffraction facets. FTIR spectra at 1,650, 2,950, and 1,400 cm À1 confirmed the capping of AgNPs with phenolic compounds and compounds having primary amines. The AgNPs showed 100 μg/ml of minimum inhibitory concentration against methicillin-resistant Staphylococcus aureus (MRSA). In addition, AgNPs showed a synergistic effect with both vancomycin and ciprofloxacin against MRSA (25%), Pseudomonas aeruginosa (50%), and pus isolated Escherichia coli (50%).Moreover, AgNPs impregnated cotton and bandage showed in vitro antibacterial potential against American Type Culture Collection and skin-associated clinical pathogenic bacteria. Findings showed that endophytic fungi are the potential source for AgNPs synthesis that are effective against multidrug-resistant bacteria and the development of antimicrobial textile finishes.

Biocompatible formulations based on mycosynthesized iron oxide nanoparticles: Fabrication, characterization, and biological investigation

“…These results explained that the higher concentration of IONPs (0.75 mg/ml) can significantly inhibit mycelial growth of pathogenic fungus Aspergillus. These results signify the potential role of IONPs in fungal‐related disease control [22]. Mycosynthesized IONPs showed a positive result against all strains of bacteria.…”

“…Myconanotechnology is a recently emerged field that describes the biosynthetic pathway of metallic and nonmetallic NPs via reduction through various organic mycomaterials present in fungi for example, mushroom, yeast, mold and so on, for various applications, particularly, in biomedical field [20][21][22].…”

Penicillium polonicum-mediated green synthesis of silver nanoparticles: Unveiling antimicrobial and seed germination advancements

“…The bacterial liquid acts as both a reducing agent and stabilizer, eliminating the need for surfactants, protective agents, or cross-linking agents during the reduction process. Previous studies have highlighted the biosynthesis of AgNPs by fungi such as Fusarium [ 39 ], Aspergillus [ 40 , 41 ], and Penicillium [ 42 , 43 ]. For instance, Fusarium oxysporum synthesized AgNPs of 5–15 nm in an aqueous Ag + solution [ 44 , 45 ].…”

Wild Mushroom (Ganoderma multipileum) as Biosource for Zinc oxide Nanoparticles: From Synthesis to Enhance Biological Applications

“…24 ., The band at 3243.24 cm -1 has been identified as O-H stretching (alcohols, phenols, aromatic primary amines a broad, strong band) of alcohol present in mushrooms. The peak at 1024.05cm has been identified as the C-N bond of the existence of primary amine in the protein [22].The peak at 1547.41 cm -1 (N-O stretching) confirms the strong (-NO3) ions from the metal precursor used in this process and the weak bond at 1411.73 cm -1 was due to out of plane bending vibration of the O-H group and indicates the attachment of carboxylic acid. The absorption peaks between 673.18 cm -1 to 526.61 cm -1 defined the C-Br bond indicate the strong attachment of halo compounds.…”