Yenilebilir mantar Macrolepiota procera kullanılarak yeşil sentez yöntemiyle sentezlenen gümüş nanopartiküllerinin in-vitro biyolojik aktivitelerinin araştırılması

Abstract

Aim: Mushrooms known that to be used in traditional treatment among the people as they can generate a large diversity of secondary metabolites. In the present study, it was aimed to synthesized silver nanoparticles (AgNPs) mediated Macrolepiota procera, which is known to have diverse biological activities such as anticancer, antioxidant, antimicrobial in previous studies, and to investigated various in-vitro biological activities of these AgNPs. Materials and Methods: Synthesized Mp-AgNPs were characterized using transmission electron microscopy (TEM). Various biological activities including antimicrobial, biofilm inhibition and cell viability inhibition, DNA cleavage, DPPH activity of synthesized AgNPs were investigated in-vitro. Results: The highest DPPH scavenging activity of Mp-AgNPs was found as 92.72%, at 200 mg/L concentration. Mp-AgNPs caused single strain break in the E. coli pBR322 plasmid DNA. Mp-AgNPs showed moderate antimicrobial activity against tested microorganisms. Furthermore, the biofilm inhibition activity of Mp-AgNPs toward P. aeruginosa and S. aureus was 65.80% and 72.60% at 200 mg/L, respectively. Inhibition activity of E. coli cell viability of Mp-AgNPs was found as 99.99% at 500 mg/L. Conclusion: From the findings obtained in the study , the use of M. procera in the AgNPs synthesis it is important private regard due to its low cost, eco-friendly, high yield and non-toxicity human health. In addition, newly synthesized AgNPs can be used effectively for different applications after further studies.

Bioinspired green-synthesized silver nanoparticles: in vitro physicochemical, antibacterial, biofilm inhibitory, genotoxicity, antidiabetic, antioxidant, and anticoagulant performance

“…After 45 min treatment of pBR322 plasmid DNA with AgNPs at concentrations of 50, 100, and 200 mg mL À1 , the agarose gel represented extensive plasmid cleavage at all tested concentrations of AgNPs, whereas no breakage was observed in the control. 43…”