This article presents the application of amperometric biosensors based on platinum printed electrodes SensLab and immobilized enzymes, alcohol oxidase, glucose oxidase, and lactate oxidase, for wine analysis. Created devices demonstrate linear response to ethanol, glucose, and lactate within the concentration range 0.3-20 mM, 0.04-2.5 mM, and 0.008-1 mM, respectively. No decrease in ethanol and glucose biosensor activity is revealed during 2 months after fabrication, and the operational stability of the lactate biosensor is sufficient only during 4 days. Developed biosensors showed high selectivity to the substrate and are successfully applied to the analysis of such complex mixtures as wine and must. Good correlation of the results of analysis of different wines and must obtained by amperometric biosensors with immobilized oxidases and traditional methods is shown. Created biosensors can be used as a basis of a commercial device for express analysis of ethanol, glucose, and lactate in wine and must during its fermentation. Application of such devices for quality control in foodstuff industry can have great economical effect because determination by biosensors is less expensive, labor-intensive, and lengthy than traditional methods of analysis.

MADICA 2006 Conference, Fifth Maghreb-Europe Meeting on Materials and their Applicatons for Devices and Physical, Chemical and Biological Sensors, MADICA 2006 Conference, Fifth Maghreb-Europe Meeting on Materials and their Applicatons for Devices and Physical, Chemical and Biological SensorsInternational audienceA lactate oxidase-based amperometric biosensor is designed for lactate determination. Two methods of lactate oxidase immobilization on the surface of commercial SensLab platinum printing electrodes are compared. The sensor with lactate oxidase immobilized by physical adsorption in Resydrol polymer is shown to have both narrower dynamic range (0.004–0.5 mМ lactate) and higher sensitivity (320 nA/mM) as compared with that immobilized in poly(3,4-ethylenedioxythiophene) by electrochemical polymerization (0.05–1.6 mM and 60 nA/mM respectively). The operational stability of the biosensors developed is studied; the immobilization method is shown to be of no influence. The lactate content in wine and in wine material during fermentation is analyzed. The data obtained by amperometric lactate biosensor correlated with those of standard chromatography. The biosensor developed can be used in food industry for control and optimization of process of wine fermentation as well as for control of wine quality

No abstract

Abstract. An investigation was performed to evaluate the effect of different zeolites (silicalite, zeolite Y and zeolite Beta with varying Si/Al ratio) on the performance of glucose amperometric biosensor based on immobilized glucose oxidase (GOD). It was observed that detection limit of biosensors based on GOD without zeolite was 0.64 mM of glucose. However, in the case of GOD immobilization with zeolites NH 4 -Beta-25, Na-Beta and silicalites, biosensors with smaller detection limits within the range of 0.01 to 0.04 mM of glucose were obtained. The study of selectivity of designed biosensors showed that biosensor based on GOD immobilized with zeolite NH 4 -Beta-25 was much selective comparing with biosensor based on GOD without zeolites. Stability of developed devises also was investigated, and in the case of biosensor with GOD and Silicalite-1 it was higher than for GOD without zeolite. Thus zeolites of different types can be effectively used for GOD immobilization in glucose amperometric biosensors development for optimization of sensitivity, selectivity and stability of these devises. Àíîòàö³ÿ. Ìåòîþ äîñë³äaeåííÿ áóëà îö³íêà âïëèâó ð³çíèõ òèï³â öåîë³ò³â (ñèë³êàë³ò³â, öå-îë³òó Y òà öåîë³ò³â Áåòà ç ð³çíèì ñï³ââ³äíîøåííÿì ñèë³ö³é-àëþì³í³é) íà ðîáî÷³ õàðàêòåðèñ-òèêè ãëþêîçíîãî àìïåðîìåòðè÷íîãî á³îñåíñîðà íà îñíîâ³ ³ììîá³ë³çîâàíî¿ ãëþêîçîîêñèäà-çè (ÃÎÄ). Áóëî âñòàíîâëåíî, ùî ãðàíèöÿ âèçíà÷åííÿ ñóáñòðàòó äëÿ á³îñåíñîðà ç ÃÎÄ áåç öåîë³ò³â ñòàíîâèòü 0,64 ìÌ ãëþêîçè, òîä³ ÿê ïðè ³ììîá³ë³çàö³¿ ÃÎÄ ç öåîë³òàìè NH 4 -Áå-òà-25, Na-Áåòà òà ñèë³êàë³òàìè áóëè îòðèìàí³ á³îñåíñîðè ç ìåíøåþ ãðàíèöåþ âèçíà÷åí-íÿ -â³ä 0,01 äî 0,04 ìÌ. Äîñë³äaeåííÿ ñåëåêòèâíîñò³ ñòâîðåíèõ á³îñåíñîð³â ïîêàçàëî, ùî ñåíñîð íà îñíîâ³ ÃÎÄ, ³ììîá³ë³çîâàíî¿ ç öåîë³òîì NH 4 -Áåòà-25, áóâ çíà÷íî ñåëåêòèâí³øèì ó ïîð³âíÿíí³ ç ñåíñîðîì íà îñíîâ³ ÃÎÄ áåç öåîë³ò³â. Òàêîae áóëî ïðîàíàë³çîâàíî ñòàá³ëü-í³ñòü ðîçðîáëåíèõ á³îñåíñîð³â òà ïîêàçàíî, ùî âîíà áóëà âèùîþ äëÿ ÃÎÄ, ³ììîá³ë³çîâàíî¿ ç Keywords: amperometric biosensor, glucose oxidase, zeolites, glucose

Àíîòàö³ÿ ðîáîò³ ïðåäñòàâëåíî äàí³ ùîäî ðîçðîáêè âèñîêî÷óòëèâîãî ñåëåêòèâíîãî êîíäóêòî-ìåòðè÷íîãî á³îñåíñîðà äëÿ âèçíà÷åííÿ ³îí³â âàaeêèõ ìåòàë³â ó âîäíèõ ðîç÷èíàõ. ßê êîí-äóêòîìåòðè÷íèé ïåðåòâîðþâà÷ âèêîðèñòîâóâàëàñÿ äèôåðåíö³éíà ïàðà ïëàíàðíèõ çîëîòèõ ãðåá³í÷àñòèõ åëåêòðîä³â, íàíåñåíèõ íà ñèòàëîâó ï³äêëàäêó. Ðîëü á³îñåëåêòèâíîãî åëåìåíòó â³ä³ãðàâàëà òðèôåðìåíòíà ñèñòåìà (³íâåðòàçà, ìóòàðîòàçà, ãëþêîçîîêñèäàçà), ³ììîá³ë³çîâà-íà íà ïîâåðõíþ ïåðåòâîðþâà÷à, ÿêà º ÷óòëèâ³øîþ ³ ñåëåêòèâí³øîþ ó ïîð³âíÿíí³ ç ³íøèìè ôåðìåíòíèìè ñèñòåìàìè çà ðàõóíîê ñóìàðíîãî åôåêòó ³íã³áóâàííÿ òðüîõ ôåðìåíò³â. Ñï³â-â³äíîøåííÿ ôåðìåíò³â â ìåìáðàí³ ï³äáèðàëè åêñïåðèìåíòàëüíî, ìàþ÷è íà ìåò³ äîñÿãíåííÿ íàéá³ëüøî¿ ÷óòëèâîñò³ á³îñåíñîðà ÿê äî ñóáñòðàòó (ñàõàðîçè), òàê ³ äî âàaeêèõ ìåòàë³â.  ðî-áîò³ âñòàíîâëåíî îïòèìàëüíèé ÷àñ ³íêóáàö³¿ á³îñåíñîðà â ðîç÷èí³ òîêñèí³â äëÿ îòðèìàííÿ íåîáõ³äíî¿ ÷óòëèâîñò³, ÿêèé ñòàíîâèâ 30 õâèëèí. Îòðèìàíî êàë³áðóâàëüí³ êðèâ³ äëÿ âèçíà-÷åííÿ ð³çíèõ ³îí³â âàaeêèõ ìåòàë³â êîíäóêòîìåòðè÷íèì á³îñåíñîðîì òà ïåðåâ³ðåíî éîãî ñå-ëåêòèâí³ñòü ùîäî ³íøèõ ãðóï òîêñèí³â. Ðîçðîáëåíèé á³îñåíñîð õàðàêòåðèçóºòüñÿ á³ëüøîþ ÷óòëèâ³ñòþ òà ñåëåêòèâí³ñòþ äî ³îí³â Hg 2+ òà Ag + ó ïîð³âíÿíí³ ç â³äîìèìè íà äàíèé ìîìåíò á³îñåíñîðàìè äëÿ àíàë³çó âàaeêèõ ìåòàë³â ³ ìîaeå áóòè ðåêîìåíäîâàíèé ïðè åêñïðåñ-àíàë³ç³ ðåàëüíèõ çðàçê³â â åêîëîã³÷íîìó ìîí³òîðèíãó.Êëþ÷îâ³ ñëîâà: êîíäóêòîìåòðè÷íèé á³îñåíñîð, ³íã³á³òîðíèé àíàë³ç, ³íâåðòàçà, ìóòàðîòà-çà, ãëþêîçîîêñèäàçà, âàaeê³ ìåòàëè The data on development of highly sensitive and selective conductometric biosensor for determination of heavy metal ions are presented. A differential pair of gold planar thin-film interdigitated electrodes deposited on the ceramic substrate was used as a conductometric transducer. As a bioselective element, the three-enzyme system (invertase, mutarotase, glucose oxidase) immobilized on the transducer surface was more sensitive and selective to heavy metal ions as compared with other enzymatic systems due to summary inhibition effect of three enzymes. The enzymes ratio in mem-

 îãëÿä³ íàâåäåíî õàðàêòåðèñòèêó ôåðìåíòíèõ á³îñåíñîð³â äëÿ ê³ëüê³ñíîãî âèçíà÷åííÿ êîìïîíåíò³â âèíà, ÿê³ áóëè ñòâîðåí³ ïðîòÿãîì îñòàíí³õ ðîê³â, òà ïðîàíàë³çîâàíî ¿õí³ îñíîâ-í³ ïåðåâàãè òà íåäîë³êè.

Aim. Development of amperometric biosensor based on L-lactate-cytochrome c-oxidoreductase (flavocytochrome b2, FC b2) for lactate determination. Methods. All experiments were performed using the amperometric method of detection. The methods of electrochemical polymerization and immobilization in glutaraldehyde vapors were used for FC b2 immobilization on the surface of electrodes. Results. The FC b2 preparation, which demonstrated the best operational characteristics after immobilization in poly (3,4-ethylen dioxythiophene), was selected. The selectivity, operational and storage stability, and pH-optimum for operation of the created biosensor were determined. The analysis of L-lactate in the model solutions and wine samples was carried outusing the developed biosensor. Conclusion. The FC b2-based biosensor due to its high stability can be effectively used for lactate determination in blood and other liquids containing no ethanol. After the selectivity optimization, the devise can be also applied for wine analysis

The work presents application of amperometric biosensors based on platinum printed electrodes and immobilized enzymes alcohol oxidase, glucose oxidase and lactate oxidase for wine analysis. Created devices demonstrate wide dynamic range of work, good stability and high selectivity to the substrate, and are successfully applied for analysis of such complex mixtures as wine and must. Good correlation of the results of analysis of different wines and must obtained by amperometric biosensors and chromatography method is shown. Created biosensors can be used as a basis of commercial device for express analysis of ethanol, glucose and lactate in wine and must during its fermentation.