Application of Amperometric Biosensors for Analysis of Ethanol, Glucose, and Lactate in Wine

Горюшкина, Т. Б.; Солдаткин, А. П.; Dzyadevych, S. V.

doi:10.1021/jf9009087

Cited by 91 publications

(40 citation statements)

References 23 publications

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“…[210][211][212][213] In addition, efforts have been made to develop enzyme microreactors as biosensors for biomedical applications (e.g., glucose detection). [214][215][216] Homogeneous catalysis. Several groups reported meso-scale batch reactors for the Baeyer-Villiger oxidation of ketones to chiral lactones.…”

Section: Biocatalytic Aerobic Oxidation Processes In Continuous Flowmentioning

confidence: 99%

Liquid phase oxidation chemistry in continuous-flow microreactors

et al. 2016

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Continuous-flow liquid phase oxidation chemistry in microreactors receives a lot of attention as the reactor provides enhanced heat and mass transfer characteristics, safe use of hazardous oxidants, high interfacial areas, and scale-up potential. In this review, an up-to-date overview of both technological and chemical aspects of liquid phase oxidation chemistry in continuous-flow microreactors is given. A description of mass and heat transfer phenomena is provided and fundamental principles are deduced which can be used to make a judicious choice for a suitable reactor. In addition, the safety aspects of continuous-flow technology are discussed. Next, oxidation chemistry in flow is discussed, including the use of oxygen, hydrogen peroxide, ozone and other oxidants in flow. Finally, the scale-up potential for continuous-flow reactors is described.

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Section: Biocatalytic Aerobic Oxidation Processes In Continuous Flowmentioning

confidence: 99%

Liquid phase oxidation chemistry in continuous-flow microreactors

et al. 2016

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“…Although the effectiveness of these systems has been demonstrated for real-time monitoring of alcoholic fermentation in industrial-scale red winemaking, the overall system was somewhat cumbersome to use. More recently, to overcome these drawbacks, Goriushkina, Soldatkin, and Dzyadevych (2009) have developed amperometric biosensors for a more convenient detection of glucose and ethanol in wine. These biosensors are based on the use of specific enzymes, such as alcohol and glucose oxidase (AO and GOD), immobilized onto the surface of bare platinum printed electrodes.…”

Section: Introductionmentioning

confidence: 99%

Real time monitoring of alcoholic fermentation with low-cost amperometric biosensors

et al. 2011

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a b s t r a c tScreen-printed glucose, ethanol and fructose biosensors, coupled with portable instrumentation, and their application to monitor micro-alcoholic fermentations (micro-ALFs) in red wine is described. For the fabrication of glucose and ethanol biosensors, graphite screen-printed sensors modified with Prussian Blue were coupled with oxidase enzymes while for the fructose biosensor, a bare screen-printed sensor was coated with fructose dehydrogenase and phenazine methansulphate was used as electrochemical mediator. The working range, reproducibility of probe fabrication and biosensor stability were all evaluated. After a recovery study, performed analysing fortified must-wine samples, the biosensors were employed to monitor micro-ALFs induced by the inoculation of two different strains of Saccharomyces cerevisiae. During the red micro-ALFs, samples of must-wine were collected and analysed by use of both biosensors and spectrophotometric kits. The data obtained demonstrated that a biosensor-based system could represent a useful tool to assist winemakers during wine production.

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“…It can be hypothesized that the Al content is important to achieve high selectivity in the current biosensor, since almost no change of selectivity was observed using silicalite samples. Selectivity is an important parameter to consider in biosensors especially for real samples [12]. Thus, silicalites are not promising candidates for this particular purpose.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, enzyme immobilization with glutaraldehyde is often used for development of enzyme biosensors. This immobilization method produces a three-dimensional matrix, in which the enzyme is closely trapped with the electrode material, thus improving both retention of the biomolecule on the electrode surface and electrical communication [12]. For formation of the glutaraldehyde-based bioselective membrane, a drop of 30% GOD solution with 5% BSA was put on the surface of working electrode.…”

Section: Enzyme Immobilization In Glutaraldehyde Vapourmentioning

confidence: 99%

Application of Zeolites for Immobilization of Glucose Oxidase in Amperometric Biosensors

Горюшкина

Akata

Sacco

et al. 2014

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Abstract. An investigation was performed to evaluate the effect of different zeolites (silicalite, zeolite Y and zeolite Beta with varying Si/Al ratio) on the performance of glucose amperometric biosensor based on immobilized glucose oxidase (GOD). It was observed that detection limit of biosensors based on GOD without zeolite was 0.64 mM of glucose. However, in the case of GOD immobilization with zeolites NH 4 -Beta-25, Na-Beta and silicalites, biosensors with smaller detection limits within the range of 0.01 to 0.04 mM of glucose were obtained. The study of selectivity of designed biosensors showed that biosensor based on GOD immobilized with zeolite NH 4 -Beta-25 was much selective comparing with biosensor based on GOD without zeolites. Stability of developed devises also was investigated, and in the case of biosensor with GOD and Silicalite-1 it was higher than for GOD without zeolite. Thus zeolites of different types can be effectively used for GOD immobilization in glucose amperometric biosensors development for optimization of sensitivity, selectivity and stability of these devises. Àíîòàö³ÿ. Ìåòîþ äîñë³äaeåííÿ áóëà îö³íêà âïëèâó ð³çíèõ òèï³â öåîë³ò³â (ñèë³êàë³ò³â, öå-îë³òó Y òà öåîë³ò³â Áåòà ç ð³çíèì ñï³ââ³äíîøåííÿì ñèë³ö³é-àëþì³í³é) íà ðîáî÷³ õàðàêòåðèñ-òèêè ãëþêîçíîãî àìïåðîìåòðè÷íîãî á³îñåíñîðà íà îñíîâ³ ³ììîá³ë³çîâàíî¿ ãëþêîçîîêñèäà-çè (ÃÎÄ). Áóëî âñòàíîâëåíî, ùî ãðàíèöÿ âèçíà÷åííÿ ñóáñòðàòó äëÿ á³îñåíñîðà ç ÃÎÄ áåç öåîë³ò³â ñòàíîâèòü 0,64 ìÌ ãëþêîçè, òîä³ ÿê ïðè ³ììîá³ë³çàö³¿ ÃÎÄ ç öåîë³òàìè NH 4 -Áå-òà-25, Na-Áåòà òà ñèë³êàë³òàìè áóëè îòðèìàí³ á³îñåíñîðè ç ìåíøåþ ãðàíèöåþ âèçíà÷åí-íÿ -â³ä 0,01 äî 0,04 ìÌ. Äîñë³äaeåííÿ ñåëåêòèâíîñò³ ñòâîðåíèõ á³îñåíñîð³â ïîêàçàëî, ùî ñåíñîð íà îñíîâ³ ÃÎÄ, ³ììîá³ë³çîâàíî¿ ç öåîë³òîì NH 4 -Áåòà-25, áóâ çíà÷íî ñåëåêòèâí³øèì ó ïîð³âíÿíí³ ç ñåíñîðîì íà îñíîâ³ ÃÎÄ áåç öåîë³ò³â. Òàêîae áóëî ïðîàíàë³çîâàíî ñòàá³ëü-í³ñòü ðîçðîáëåíèõ á³îñåíñîð³â òà ïîêàçàíî, ùî âîíà áóëà âèùîþ äëÿ ÃÎÄ, ³ììîá³ë³çîâàíî¿ ç Keywords: amperometric biosensor, glucose oxidase, zeolites, glucose

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Application of Amperometric Biosensors for Analysis of Ethanol, Glucose, and Lactate in Wine

Cited by 91 publications

References 23 publications

Liquid phase oxidation chemistry in continuous-flow microreactors

Liquid phase oxidation chemistry in continuous-flow microreactors

Real time monitoring of alcoholic fermentation with low-cost amperometric biosensors

Application of Zeolites for Immobilization of Glucose Oxidase in Amperometric Biosensors

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