Application of Zeolites for Immobilization of Glucose Oxidase in Amperometric Biosensors

Abstract

Abstract. An investigation was performed to evaluate the effect of different zeolites (silicalite, zeolite Y and zeolite Beta with varying Si/Al ratio) on the performance of glucose amperometric biosensor based on immobilized glucose oxidase (GOD). It was observed that detection limit of biosensors based on GOD without zeolite was 0.64 mM of glucose. However, in the case of GOD immobilization with zeolites NH 4 -Beta-25, Na-Beta and silicalites, biosensors with smaller detection limits within the range of 0.01 to 0.04 mM of glucose were obtained. The study of selectivity of designed biosensors showed that biosensor based on GOD immobilized with zeolite NH 4 -Beta-25 was much selective comparing with biosensor based on GOD without zeolites. Stability of developed devises also was investigated, and in the case of biosensor with GOD and Silicalite-1 it was higher than for GOD without zeolite. Thus zeolites of different types can be effectively used for GOD immobilization in glucose amperometric biosensors development for optimization of sensitivity, selectivity and stability of these devises. Àíîòàö³ÿ. Ìåòîþ äîñë³äaeåííÿ áóëà îö³íêà âïëèâó ð³çíèõ òèï³â öåîë³ò³â (ñèë³êàë³ò³â, öå-îë³òó Y òà öåîë³ò³â Áåòà ç ð³çíèì ñï³ââ³äíîøåííÿì ñèë³ö³é-àëþì³í³é) íà ðîáî÷³ õàðàêòåðèñ-òèêè ãëþêîçíîãî àìïåðîìåòðè÷íîãî á³îñåíñîðà íà îñíîâ³ ³ììîá³ë³çîâàíî¿ ãëþêîçîîêñèäà-çè (ÃÎÄ). Áóëî âñòàíîâëåíî, ùî ãðàíèöÿ âèçíà÷åííÿ ñóáñòðàòó äëÿ á³îñåíñîðà ç ÃÎÄ áåç öåîë³ò³â ñòàíîâèòü 0,64 ìÌ ãëþêîçè, òîä³ ÿê ïðè ³ììîá³ë³çàö³¿ ÃÎÄ ç öåîë³òàìè NH 4 -Áå-òà-25, Na-Áåòà òà ñèë³êàë³òàìè áóëè îòðèìàí³ á³îñåíñîðè ç ìåíøåþ ãðàíèöåþ âèçíà÷åí-íÿ -â³ä 0,01 äî 0,04 ìÌ. Äîñë³äaeåííÿ ñåëåêòèâíîñò³ ñòâîðåíèõ á³îñåíñîð³â ïîêàçàëî, ùî ñåíñîð íà îñíîâ³ ÃÎÄ, ³ììîá³ë³çîâàíî¿ ç öåîë³òîì NH 4 -Áåòà-25, áóâ çíà÷íî ñåëåêòèâí³øèì ó ïîð³âíÿíí³ ç ñåíñîðîì íà îñíîâ³ ÃÎÄ áåç öåîë³ò³â. Òàêîae áóëî ïðîàíàë³çîâàíî ñòàá³ëü-í³ñòü ðîçðîáëåíèõ á³îñåíñîð³â òà ïîêàçàíî, ùî âîíà áóëà âèùîþ äëÿ ÃÎÄ, ³ììîá³ë³çîâàíî¿ ç Keywords: amperometric biosensor, glucose oxidase, zeolites, glucose

Study of zeolite influence on analytical characteristics of urea biosensor based on ion-selective field-effect transistors

“…Finally, zeolites are known to be stable in both wet and dry conditions and well-tolerated by microorganisms, leading to an enhanced compatibility with biochemical analyses. All of these properties make zeolites unique nanomaterials and promising candidates for the immobilization of biological molecules and for advanced analytical tasks [5]. …”

Elaboration of new method of enzyme adsorption on silicalite and nano beta zeolite for amperometric biosensor creation

“…Some of them, which are formed due to barely perceptible heat and pressure, can be called metamorphic only conditionally whereas others are found in obviously metamorphic regimes. Zeolites are of great interest due to their large surface areas, rigid and well defined pore structures, thermal stability, and tailorable surface charges with respect to other types of nanomaterials [4]. Particularly, the nanosized pores of zeolites can be adjusted to precisely determined uniform openings allowing the molecules smaller than the pores diameter to be adsorbed.…”

“…In [6][7][8] the use of zeolite in the biosensor structure has been shown to increase the sensitivity and improve the selectivity. As demonstrated in [4], zeolites of various kinds can be effectively applied for the glucose oxidase immobilization while developing glucose amperometric biosensors to optimize their sensitivity, selectivity and stability. It was also shown that zeolites can be a basis for the creation of a new type of amperometric biosensors without mediators since zeolites can serve as charge carriers [7,9].…”

“…The promising results were obtained when clinoptilolite was used at the development of enzyme biosensors based on pH-sensitive field-effect transistors [15][16][17][18]. The basic idea was to attain high sensitivity of the potentiometric transducer to NH 4 + by deposition of a clinoptilolite layer on the transducer surface [15]. The described effect was also observed in the biosensors based on urease [17] and urease co-immobilized with arginase [18].…”

Nano- and microsized zeolites as a perspective material for potentiometric biosensors creation

“…As shown in [14,15], zeolites of various kinds can be effectively used for the glucose oxidase immobilization when developing glucose amperometric biosensors to optimize their sensitivity, selectivity, and stability. As reported in [10,16,17], zeolites are used as alternative carriers for the enzyme immobilization at the creation of conductometric biosensors.…”