Nano- and microsized zeolites as a perspective material for potentiometric biosensors creation

Abstract

A number of potentiometric biosensors based on coimmobilization of enzymes with different types of zeolite on pH-ion-sensitive field-effect transistor (ISFET) have been developed. Their working characteristics have been determined and compared.It was shown that clinoptilolite and zeolite Beta polymorph A (BEA) are more promising for creating biosensors than zeolite A. Changing the concentration of zeolite BEA in membranes, it is possible to extend the biosensor linear measurement range. The two-layer method of deposition of the enzyme with clinoptilolite was found to provide a significant increase in the biosensor sensitivity to substrates, whereas thermal modification of the zeolite BEA crystals can improve analytical characteristics of potentiometric biosensors for detection of toxic substances.These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via different zeolites. This makes zeolites strong candidates for integration into biosensors as ISFET modifiers.

A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

“…The procedure of enzyme adsorption on silicalite was tested for a number of enzymes—acetylcholinesterase [27], urease [23, 25], recombinant urease [26], and butyrylcholinesterase [27]. Additionally, the effectiveness of this technique has been shown for enzyme biosensors based on pH-sensitive field-effect transistors [28–32]. Moreover, it was revealed that some zeolites can be useful for glucose oxidase adsorption in amperometric biosensors for glucose determination [33].…”

Properties and applications of natural zeolites

“…Other references are provided: Porada et al 2023;Akbelen and Ösbay, 2022;Bayrakdar, 2022;Chang et al 2022;Cadar et al 2020;Wattanawong et al 2020;Gil and Vicente, 2019;Pinto-Santamaría et al 2019;Tamiji and Nezamzadeh-Ejhieh, 201 9;Chmielewská et al 2018;Souza et al 2018;Niknezhadi and Nezamzadeh-Ejhieh, 2017;Bruffey et al 2016;de Araújo, 2016;Favvas et al 2016;Griffith et al 2015;Locati et al 2015;Soldatkin et al 2015Vasylechko et al 2015, Ulmanu and Anger, 2012, Zorpas, 2012, Armbruster, 2001, Tchernev, 1993. Abbondanzi, F., Faccini, B., Di Giuseppe, D., Malferrari, D., Coltorti, M., & Laurora, A.…”

Photophysical Properties of Silver Clusters in Faujasite Zeolites: Does the Crystal Size Matter?

“…The external surface charge of the zeolite nanoparticles in particular has been shown to play an important role in many interactions . For instance, nanosized zeolites have been used as multifunctional nanocarriers to deliver organic molecules into living cells where the drug of interest is attached to the external surfaces. − …”