Elaboration of new method of enzyme adsorption on silicalite and nano beta zeolite for amperometric biosensor creation

Солдаткін, О. О.; Kasap, Berna Ozansoy; Akata, Burcu; Солдаткин, А. П.; Dzyadevych, S. V.; El’skaya, A. V.

doi:10.7124/bc.0008a3

Cited by 11 publications

(9 citation statements)

References 21 publications

(31 reference statements)

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“…Additionally, the effectiveness of this technique has been shown for enzyme biosensors based on pH-sensitive field-effect transistors [28–32]. Moreover, it was revealed that some zeolites can be useful for glucose oxidase adsorption in amperometric biosensors for glucose determination [33]. Therefore, an attempt was made to apply this method of immobilization for the development of amperometric glutamate-sensitive biosensor with improved analytical characteristics using GlOx adsorbed on silicalite.…”

Section: Introductionmentioning

confidence: 99%

A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

et al. 2017

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In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples.

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Section: Introductionmentioning

confidence: 99%

A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

et al. 2017

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“…An idea of enzyme adsorption on zeolites has been proposed in previous work [ 5 ]. Currently, a number of biosensors based on various types of zeolites have been developed using different enzyme systems: urease [ 6 – 11 ], glucose oxidase [ 12 – 14 ], acetylcholinesterase [ 15 ], butyrylcholinesterase [ 16 , 17 ], invertase/mutarotase/glucose oxidase [ 18 ], etc.…”

Section: Introductionmentioning

confidence: 99%

Creatinine Deiminase Adsorption onto Silicalite-Modified pH-FET for Creation of New Creatinine-Sensitive Biosensor

et al. 2016

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In the work, silicalite particles were used for the surface modification of pH-sensitive field-effect transistors (pH-FETs) with the purpose of developing new creatinine-sensitive biosensor. Creatinine deiminase (CD) adsorbed on the surface of silicalite-coated pH-FET served as a bioselective membrane. The biosensor based on CD immobilized in glutaraldehyde vapor (GA) was taken as control. The creatinine-sensitive biosensor obtained by adsorption on silicalite was shown to have better analytical characteristics (two- to threefold increased sensitivity to creatinine, three- to fourfold lesser response and recovery times, a decrease of the detection limit of creatinine determination to 5 μM, etc.).Additionally, the biosensor based on CD adsorbed on silicalite (Sil/CD) was characterized by high signal reproducibility (relative standard deviation (RSD) for creatinine measurement = 2.6 %) and stability during storage (over 13 months). It was experimentally confirmed that the proposed biosensor was not sensitive either to high concentrations of sodium chloride or to the macromolecular protein fractions and can be used for direct quantitative analysis of creatinine in the blood serum.It was concluded that the method of CD adsorption on silicalite is well-suited for the creation of creatinine-sensitive biosensor with improved working characteristics.

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“…As shown in [14,15], zeolites of various kinds can be effectively used for the glucose oxidase immobilization when developing glucose amperometric biosensors to optimize their sensitivity, selectivity, and stability. As reported in [10,16,17], zeolites are used as alternative carriers for the enzyme immobilization at the creation of conductometric biosensors.…”

Section: Introductionmentioning

confidence: 99%

Nano- and microsized zeolites as a perspective material for potentiometric biosensors creation

et al. 2015

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A number of potentiometric biosensors based on coimmobilization of enzymes with different types of zeolite on pH-ion-sensitive field-effect transistor (ISFET) have been developed. Their working characteristics have been determined and compared.It was shown that clinoptilolite and zeolite Beta polymorph A (BEA) are more promising for creating biosensors than zeolite A. Changing the concentration of zeolite BEA in membranes, it is possible to extend the biosensor linear measurement range. The two-layer method of deposition of the enzyme with clinoptilolite was found to provide a significant increase in the biosensor sensitivity to substrates, whereas thermal modification of the zeolite BEA crystals can improve analytical characteristics of potentiometric biosensors for detection of toxic substances.These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via different zeolites. This makes zeolites strong candidates for integration into biosensors as ISFET modifiers.

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Elaboration of new method of enzyme adsorption on silicalite and nano beta zeolite for amperometric biosensor creation

Abstract: Aim. Optimization of a new method of enzyme immobilization

Cited by 11 publications

References 21 publications

A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

Creatinine Deiminase Adsorption onto Silicalite-Modified pH-FET for Creation of New Creatinine-Sensitive Biosensor

Nano- and microsized zeolites as a perspective material for potentiometric biosensors creation

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