Enzymatic Biosensors for Quantitative Analysis of Wine`s Components

Abstract

 îãëÿä³ íàâåäåíî õàðàêòåðèñòèêó ôåðìåíòíèõ á³îñåíñîð³â äëÿ ê³ëüê³ñíîãî âèçíà÷åííÿ êîìïîíåíò³â âèíà, ÿê³ áóëè ñòâîðåí³ ïðîòÿãîì îñòàíí³õ ðîê³â, òà ïðîàíàë³çîâàíî ¿õí³ îñíîâ-í³ ïåðåâàãè òà íåäîë³êè.

The procedure of ethanol determination in wine by enzyme amperometric biosensor

“…Tak ing into con sid er ation the above stated, the enzyme amperometric biosensors seem to be at trac tive alter na tive to the tra di tional an a lyt i cal meth ods and, thus, their ap pli ca tion is one of the most pro spec tive approaches to solv ing the prob lems of fast, cheap and re liable de ter mi na tion of eth a nol in com plex mix turesmust and wines [4]. There fore, dur ing last years the biosensors are more and more widely used for the analy sis of pro duc tion qual ity in food industry including winemaking [9].…”

“…Be sides, an in crease in eth anol con cen tra tion in the must has a neg a tive ef fect on yeast growth and de vel op ment dur ing wine fer men tation [1]. There fore, per ma nent eth a nol mea sure ment, along with an anal y sis of other key com po nents, is neces sary from eco nom i cal viewpoint to optimize fermentation process and prevent its disorder [1,4].…”

“…AO is an oligomeric block con sist ing of eight iden ti cal sub units or ga nized in quasi-cu bical sys tem, each of which con tains strongly bound co fac tor PAD [10]. Ow ing to this, there is no need to in tro duce the co fac tor ex og e nously that sim plifies an an a lyt i cal pro ce dure [4]. The de vel oped amperometric biosensors based on im mo bi lized AO, have wide dy namic range from 0.1 to 30 mM of the substrate con cen tra tion [10], which well cor re sponds to the vari a tion of ethanol concentration in wines and wine materials.…”

“…How ever, nu mer ous AO based amperometric biosensors de vel oped up to date are to some ex tent disad van ta geous com pared to the tra di tional meth ods of eth a nol anal y sis in wine. The most im por tant chal lenge is their low sta bil ity and in suf fi cient selectivity [4].…”

“…Note wor thy, that in [13,14] the AO im mo bi li za tion on the sur face of plat i num elec trodes was car ried out elec tro chem i cally in os mium-con tain ing [13] and ac ryl [14] poly mers, in [12] AO was im mo bi lized in polycarbamoylsulphite hydrogel, in [15,16] -on carbon elec trodes in glutaraldehyde. For the mo ment, it has been es tab lished that en zyme sta bil ity de pends, along with other fac tors, on a method of its im mo bi li zation on the elec trode sur face, an ef fect of sen si tive mem brane com po nents on the en zyme func tion and selec tion of the elec trode ma te rial [4]. As shown in [17], the AO-based bio sen sor with gold elec trodes and the en zyme, im mo bi lized by cross-link ing with BSA in glutaraldehyde, demonstrated almost 100% activity after 90 measurements.…”